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Preparation method of quantum dot/enzyme compounded carbon paste electrode for detecting SAM (S-Adenosylmethionine)

A technology of quantum dots and composite carbon, which is applied in the field of preparation of quantum dots/enzyme composite carbon paste electrodes, to achieve the effects of easy surface, surface renewal, and small residual current

Inactive Publication Date: 2015-10-07
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The bio-enzyme electrode sensor is currently developed with the characteristics of specificity, stability, fast detection speed, good selectivity, and high sensitivity. It is widely used in the fields of clinical medicine, food, environment, and biological sample detection. The detection of SAM on carbon paste electrode has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of semiconductor precursor solution: In the reactor, add 1.0g of tellurium powder, 0.9g of sodium borohydride, 50mL of deionized water, and ultrasonically disperse for 10 min under the protection of argon, and keep the temperature at 60°C Reflux the reaction for 5 hours, cool to room temperature, and place in an ice-water bath to obtain a semiconductor precursor solution;

[0030] (2) Preparation of zinc and cadmium precursor liquid: In the reactor, add cadmium chloride: 0.6g, zinc chloride: 0.5g, cysteine: 0.9g, water: 98mL, and adjust the pH of the solution with sodium hydroxide was 10, introduced argon, refluxed at 85°C for 4 hours, and cooled to room temperature to obtain a zinc-cadmium precursor liquid;

[0031] (3) Preparation of water-soluble CdZnTe quantum dots: Add the semiconductor precursor solution prepared in step (1) into the zinc-cadmium precursor solution prepared in step (2) under the protection of argon, and react at 85°C for 5 hours un...

Embodiment 2

[0035] (1) Preparation of semiconductor precursor solution: In the reactor, add 0.5g of tellurium powder, 0.6g of sodium borohydride, 30mL of deionized water, and ultrasonically disperse for 10 min under the protection of argon, and keep the temperature at 60°C Reflux the reaction for 4 hours, cool to room temperature, and place in an ice-water bath to obtain a semiconductor precursor solution;

[0036] (2) Preparation of zinc and cadmium precursor liquid: In the reactor, add cadmium chloride: 1.0g, zinc chloride: 0.8g, cysteine: 1.2g, water: 97mL, and adjust the pH of the solution with sodium hydroxide was 10, passed in argon, refluxed at 80°C for 5 hours, and cooled to room temperature to obtain a zinc-cadmium precursor liquid;

[0037] (3) Preparation of water-soluble CdZnTe quantum dots: add the semiconductor precursor solution prepared in step (1) to the zinc-cadmium precursor solution prepared in step (2) under the protection of argon, and react at 85°C for 4 hours under...

Embodiment 3

[0041] (1) Preparation of semiconductor precursor solution: In the reactor, add 0.8g of tellurium powder, 0.5g of sodium borohydride, 40mL of deionized water, and ultrasonically disperse for 10 min under the protection of argon, and keep the temperature at 60°C Reflux the reaction for 6 hours, cool to room temperature, and place in an ice-water bath to obtain a semiconductor precursor solution;

[0042] (2) Preparation of zinc and cadmium precursor liquid: In the reactor, add cadmium chloride: 0.2g, zinc chloride: 0.2g, cysteine: 0.6g, water: 99mL, and adjust the pH of the solution with sodium hydroxide was 10, introduced argon, refluxed at 85°C for 4 hours, and cooled to room temperature to obtain a zinc-cadmium precursor liquid;

[0043] (3) Preparation of water-soluble CdZnTe quantum dots: Add the semiconductor precursor solution prepared in step (1) into the zinc-cadmium precursor solution prepared in step (2) under the protection of argon, and react at 85°C for 6 hours un...

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Abstract

The invention discloses a preparation method of a quantum dot / enzyme compounded carbon paste electrode for detecting SAM (S-Adenosylmethionine). The preparation method is characterized by comprising the following steps of doping water-soluble CdZnTe quantum dots and transmethylase in a carbon paste electrode; by taking 1-ethyl-3--methylimidazolium hexafluorophosphate as an adhesive, mixing a carbon nano-tube with graphite powder to prepare the carbon electrode, so as to obtain the quantum dot / enzyme compounded carbon paste electrode; compared with an ordinary carbon paste electrode, the quantum dot / enzyme compounded carbon paste electrode has the advantages of conducting property improved by 1 to 2 times, wide electrochemical window, simple preparation method, low cost, easy update for surface, low residual current and the like; the eelctrode can be used for quickly detecting the SAM in a sample; the method has the characteristics of high sensitivity, good selectivity, short response time and low interference, is superior to other detection methods, and is a determination method with simpleness, quickness, convenience and feasibility for the SAM. A fixed methyltransferase electrode sensor prepared by the preparation method disclosed by the invention is low in cost, simple in preparation process and good in specificity, and has the possibility of realizing automated field determination.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method and application of a quantum dot / enzyme composite carbon paste electrode for detecting S-adenosylmethionine (SAM). Background technique [0002] S-adenosylmethionine (SAM), SAM contains active methyl groups, and almost all the methyl groups used for methylation modification in cells come from SAM methyl-thio high-energy bonds. Due to the extensiveness of the methylation reaction, it can be said that SAM is a coenzyme that is second only to ATP in the importance of participating in the reaction in the cell. A small change in the concentration of SAM in the cell will have a significant impact on the growth, differentiation and function of the cell. influences. SAM is mainly synthesized in bacteria by SAM synthase (MetK) through methionine (Met) and ATP. when E. coli The level of SAM synthetase decreases, resulting in the lack of methyl don...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 李慧芝卢燕赵淑英
Owner UNIV OF JINAN
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