Preparation method of hyaluronidase modified vanadium nitride paste electrode sensor

A technology of hyaluronidase and electrode sensor is applied in the field of electrochemical analysis, which can solve the problems of low capacity and ineffective utilization of area, and achieve the effects of wide electrochemical window, low cost and simple preparation method.

Inactive Publication Date: 2018-08-17
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The capacity of single-layer graphene can reach 21μF / cm 2 , but in many cases graphene is stacked with multiple layers, the area between layers is not effectively utilized, and its actual capacity is lower than that of single-layer graphene. Combining graphene with other nanostructures also inhibits the development of graphene layer overlap

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of nano-VN / graphene composite paste electrode: In an agate mortar, add nano-VN: 48g, graphene oxide: 16g, N-ethyl-3-methylpyridine hexafluorophosphate: 12g , liquid paraffin: 12g, petroleum ether: 18 mL, grind evenly to obtain carbon paste of nano-VN / graphene / N-ethyl-3-methylpyridine hexafluorophosphate mixture; then put the carbon paste into the The inner diameter of the wire is Φ3mm in a glass tube, compacted, dried, polished with polishing powder, polished, and washed with deionized water to obtain a nano-VN / graphene composite paste electrode;

[0029](2) Preparation of nano-VN / graphene composite paste electrode modified by γ-glycidyloxypropyltrimethoxysilane: in the reactor, add absolute ethanol: 89 mL, γ-glycidyloxypropyltrimethoxysilane Oxysilane: 30g, mix well, put the nano-VN / graphene composite paste electrode into it, react at room temperature for 2h, raise the temperature to 55±2°C for 4h, take out the electrode, wash with absolute ethanol, dry...

Embodiment 2

[0033] (1) Preparation of nano-VN / graphene composite paste electrode: In an agate mortar, add nano-VN: 50g, graphene oxide: 15g, N-ethyl-3-methylpyridine hexafluorophosphate: 110g , liquid paraffin: 11g, petroleum ether: 20mL, grind evenly to get nano VN / graphene / N-ethyl-3-methylpyridine hexafluorophosphate mixture carbon paste; then put the carbon paste into the wire In a glass tube with an inner diameter of Φ3mm, compact it, dry it, grind it with polishing powder, polish it, and wash it with deionized water to get a nano-VN / graphene composite paste electrode;

[0034] (2) Preparation of nano VN / graphene composite paste electrode modified by γ-glycidyloxypropyltrimethoxysilane: in the reactor, add absolute ethanol: 86mL, γ-glycidyloxypropyltrimethoxy Base silane: 32g, mix well, put the nano-VN / graphene composite paste electrode into it, react at room temperature for 2h, raise the temperature to 55±2°C for 4h, take out the electrode, wash with absolute ethanol, dry to get γ-sh...

Embodiment 3

[0038] (1) Preparation of nano-VN / graphene composite paste electrode: In an agate mortar, add nano-VN: 46g, graphene oxide: 18g, N-ethyl-3-methylpyridine hexafluorophosphate: 14g , liquid paraffin: 10g, petroleum ether: 15 mL, grind evenly to obtain carbon paste of nano-VN / graphene / N-ethyl-3-methylpyridine hexafluorophosphate mixture; then put the carbon paste into the The inner diameter of the wire is Φ3mm in a glass tube, compacted, dried, polished with polishing powder, polished, and washed with deionized water to obtain a nano-VN / graphene composite paste electrode;

[0039] (2) Preparation of γ-glycidyloxypropyltrimethoxysilane modified nano-VN / graphene composite paste electrode: In the reactor, add absolute ethanol: 91mL, γ-glycidyloxypropyltrimethoxy Base silane: 28g, mix well, put the nano-VN / graphene composite paste electrode into it, react at room temperature for 2h, raise the temperature to 55±2°C for 4h, take out the electrode, wash with absolute ethanol, dry to get...

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PUM

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Abstract

The invention discloses a preparation method of a hyaluronidase modified vanadium nitride paste electrode sensor. The preparation method is characterized by comprising the following steps: preparing anano VN/graphene composite paste electrode from nano VN, graphene oxide, N-ethyl-3-methylpyridine hexafluorophosphate; then, modifying the nano VN/graphene composite paste electrode with gamma-glycidyl ether oxopropyl trimethoxy silane to obtain a gamma-glycidyl ether oxopropyl trimethoxy silane modified nano VN/graphene composite paste electrode; preparing hyaluronidase stationary liquid from deionized water, bovine serum albumin, hyaluronidase and polyvinyl alcohol; finally, dropwise applying the hyaluronidase stationary liquid to the gamma-glycidyl ether oxopropyl trimethoxy silane modified nano VN/graphene composite paste electrode, and drying to obtain the hyaluronidase modified vanadium nitride paste electrode sensor. With higher electrical conductivity than a common carbon paste electrode, the electrode sensor is specific to hyaluronic acid and has high sensitivity.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method and application of a vanadium nitride paste electrode sensor modified by hyaluronidase. It belongs to the field of electrochemical analysis. Background technique [0002] Hyaluronic acid is an acidic mucopolysaccharide. In 1934, Meyer, a professor of ophthalmology at Columbia University in the United States, first isolated the substance from the bovine vitreous. With its unique molecular structure and physical and chemical properties, hyaluronic acid shows a variety of important physiological functions in the body, such as lubricating joints, regulating the permeability of blood vessel walls, regulating protein, water and electrolyte diffusion and operation, and promoting wound healing. More importantly, hyaluronic acid has a special water-retaining effect and is the most moisturizing substance found in nature so far. It is known as an ide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/3271
Inventor 李慧芝卢燕李志英
Owner UNIV OF JINAN
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