Method for detecting copper ions with 1,4-dyhydroxy-9,10-anthraquinone salicylic hydrazide compound as fluorescence probe

A technology of anthraquinone salicylic hydrazide and fluorescent probe, applied in the field of optical analysis and detection, can solve the problems of increasing immune function and the like

Inactive Publication Date: 2016-01-06
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, anthraquinones also have the effects of hemostasis and blood circulation, promoting sleep, reducing blood lipids, and increasing immune function. However, there is no relevant report on the use of anthraquinones as fluorescent probes for the detection of copper ions

Method used

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  • Method for detecting copper ions with 1,4-dyhydroxy-9,10-anthraquinone salicylic hydrazide compound as fluorescence probe
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  • Method for detecting copper ions with 1,4-dyhydroxy-9,10-anthraquinone salicylic hydrazide compound as fluorescence probe

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Experimental program
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Embodiment 1

[0026] Optimization of Experimental Conditions

[0027] 1. Selection of probe molecule concentration

[0028] Increasing the concentration of probe molecules can increase the absorbance and fluorescence intensity, which is beneficial to improve the selectivity of the determination of metal ions and the linear range of the standard curve. However, when the concentration is too high, the fluorescence intensity of the blank will also increase with the increase of the probe molecule concentration, and if the concentration is too low, it will be difficult to detect the fluorescence absorption peak after quenching by adding ions. Considering the sensitivity and linear range of the method, The concentration of the probe was chosen to be 1.0×10 -5 mol / L.

[0029] 2. Selection of buffer solution

[0030] Add 1mL phosphate buffer solution to a 10mL colorimetric tube, then add 0.01mL molar concentration of 1.0×10 -3 mol / L of 1,4-dihydroxy-9,10-anthraquinone salicylhydrazide in DMF-H ...

Embodiment 2

[0037] Add 1mL of phosphate buffer solution to several 10mL colorimetric tubes, and then add 0.01mL of the molar concentration to 1.0×10 -3 mol / L of 1,4-dihydroxy-9,10-anthraquinone salicylic hydrazide in DMF-H 2 O (DMF and H 2 The volume ratio of O is 1:99) solution, and then add copper ion solutions with different concentrations ([Cu 2+ ](×10 -6mol / L): 2, 2.5, 3.0, 3.5, 4, 4.5, 5, 5.5, 6, 8, 10), dilute to 10mL with deionized water, shake well and mix for 25min, measure the fluorescence intensity of each solution, Fluorescence intensity measurement conditions are excitation slit 10nm, emission slit 10nm, excitation wavelength 498nm, emission wavelength 575nm, and the solution changes from light red to blue purple during the measurement process. image 3 It is the fluorescence absorption spectrum diagram of copper ion solutions with different concentrations. It can be seen from the figure that the fluorescence absorption peak at 498nm shifts red and a new fluorescence abso...

Embodiment 3

[0041] Add 1mL of phosphate buffer solution to several 10mL colorimetric tubes, and then add 0.01mL of the molar concentration to 1.0×10 -3 mol / L of 1,4-dihydroxy-9,10-anthraquinone salicylic hydrazide in DMF-H 2 O solution, and then add the molar concentration of 1.0×10 -3 mol / L metal ion standard solution, dilute to 10mL with deionized water, shake well and measure the fluorescence intensity of each solution (such as Figure 5 ), the results show that when various metal ions of the same concentration interact with the probe molecule EXZ, only Cu 2+ The addition of the mixed system can reduce the fluorescence intensity at 575nm and cause fluorescence quenching, but when other metal ions interact with the probe molecules, compared with the blank sample, the fluorescence intensity does not change significantly. It shows that the probe molecule EXZ has good selectivity in recognizing copper ions.

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Abstract

The invention discloses a method for detecting copper ions with the 1,4-dyhydroxy-9,10-anthraquinone salicylic hydrazide compound as a fluorescence probe and belongs to the technical field of optical analysis detection. According to the method, the recognition action of the 1,4-dyhydroxy-9,10-anthraquinone salicylic hydrazide and 15 types of metal ions is observed and researched through experiments, it is found out that the probe molecule EXZ has a good selective recognition action on the copper ions, the probe molecule can achieve fluorescent recognition of the copper ions in a water solution through the solubleness of hydroxide radical and water, and a good result can be achieved under the physiological pH value condition. The method is environmentally friendly, the selectivity of the copper ions is concentrated, sensitivity is high, a fluorescence quenching function is achieved within a short reaction time, and the probe molecule can be successfully applied to detection of the copper ions in creature living cells.

Description

technical field [0001] The invention belongs to the technical field of optical analysis and detection, and in particular relates to a method for detecting copper ions by using a 1,4-dihydroxy-9,10-anthraquinone salicylazide compound as a fluorescent probe. Background technique [0002] Copper is an essential trace element for the human body. It is widely distributed in biological tissues, most of which exist in organic complexes, many of which are metalloproteins, and play a functional role in the form of enzymes. These enzymes are crucial to life processes. Studies have shown that copper deficiency can lead to various diseases, but excessive copper concentration can also lead to adverse health effects, such as cytotoxicity, liver and kidney damage, and neurodegenerative diseases. In addition, copper is also one of the common heavy metal pollutants in the environment. Therefore, it is of great significance to establish an accurate and sensitive detection method for copper i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 时蕾王振平刘统信麻娜娜张贵生刘青峰
Owner HENAN NORMAL UNIV
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