Method for detecting benzoyl peroxide content of flour simply, conveniently and quickly

Abstract

The invention provides a method for detecting the benzoyl peroxide content of flour simply, conveniently and quickly. The method comprises the following steps of: (1) adding different amounts of the benzoyl peroxide into mixed solution of N-methoxyl rhodamine lactam shown as a formula (I) and ferrous chloride to react to obtain reaction solution, irradiating the reaction solution by using exciting light with wavelength of 515 nm, and measuring the fluorescence intensity of the reaction solution at the position of wavelength of 555 nm to obtain a linear equation between the benzoyl peroxide concentration of the mixed solution and the variation value of the fluorescence intensity of the reaction solution, wherein the variation value of the fluorescence intensity of the reaction solution is the differential value of the fluorescence intensity of the reaction solution and the fluorescence intensity of the mixed solution; and (2) adding a benzoyl-peroxide-containing sample to be tested into the mixed solution of the N-methoxyl rhodamine lactam shown as the formula (I) and the ferrous chloride to react to obtain reaction solution a, irradiating the reaction solution a by using the exciting light with wavelength of 515 nm, measuring the fluorescence intensity of the reaction solution a at the position of wavelength of 555 nm to obtain the fluorescence intensity of the reaction solution a so as to obtain the differential value of the fluorescence intensity of the reaction solution a and the fluorescence intensity of the mixed solution, and obtaining the benzoyl peroxide concentration of the mixed solution according to a linear equation so as to obtain the benzoyl peroxide content of the sample to be tested. The method is high in measurement accuracy in a certain range, has theadvantages of simpleness and convenience in operation, low cost, quickness, high efficiency and the like, and is easy to popularize and apply.

Description

technical field [0001] The invention relates to a simple and fast method for detecting the content of benzoyl peroxide in flour, which belongs to the technical field of fluorescence detection. Background technique [0002] Benzoyl peroxide (BPO or BP) as a whitening agent has been widely used in the bleaching of wheat flour. The basic principle is that benzoyl peroxide can oxidize and destroy the conjugated double bonds of carotenoids in wheat flour, so that the color of wheat flour can be improved and improved. However, excessive addition of benzoyl peroxide not only affects the taste of flour products, but also produces a large amount of decomposition products of benzoyl peroxide, which is not conducive to human health. Since May 1, 2011, the Ministry of Health of the People's Republic of China has banned the addition of benzoyl peroxide in flour production. [0003] At present, various methods for detecting benzoyl peroxide content in flour mainly include high performan...

AI Technical Summary

Problems solved by technology

However, there are few reports on the detection of benzoyl peroxide in flour by fluorescence method

Images