1 results about "Cell Adhesion Inhibition" patented technology

This invention discloses a gas shear-based microfluidic droplet preparation device for cell culture, belonging to the fields of biomedical engineering and cell culture technology. The device includes a microfluidic chip body fixed on a substrate, a sample inflow module, a gas inflow module, a droplet generation module composed of coaxially arranged sample and gas channels, and a biocompatible solidification and collection pool. This invention utilizes gas shear to avoid the toxicity of organic solvents, and the collection pool contains a mixture of culture medium and solidifying agent, achieving integrated droplet generation, solidification, and culture, thus reducing the risk of contamination. Through channel size control, inner wall cell adhesion inhibition, and multilayer coaxial structure design, it is possible to prepare cell microsphere carriers with uniform size, controllable diameter (150-400 μm), and cell viability exceeding 93%, showing broad application prospects in three-dimensional cell culture, tissue engineering, and regenerative medicine.