2 results about "Cell Adhesion Inhibition" patented technology

An object of the present invention is to provide a cell culture substrate with laminated cardiomyocyte sheets for evaluating a curative effect by biological transplantation of a normal cardiomyocyte sheet and a method for producing the same. A further object of the present invention is to provide a method for evaluating a curative effect of a normal cardiomyocyte sheet for biological transplantation. The cell culture substrate with laminated cardiomyocyte sheets of the present invention includes two or more layers of cardiomyocyte sheets on a cell culture substrate, wherein the cell culture substrate includes an alternating arrangement portion in which a strip-shaped cell adhesion region and a strip-shaped cell adhesion suppression region are alternately arranged, the cell culture substrate with laminated cardiomyocyte sheets includes the two or more layers of cardiomyocyte sheets on at least the alternating arrangement portion of the cell culture substrate; and the cell culture substrate with laminated cardiomyocyte sheets includes, as the cardiomyocyte sheets, at least one layer of each of a disease cardiomyocyte sheet containing a differentiation-induced cardiomyocyte derived from a disease patient and a normal cardiomyocyte sheet containing a differentiation-induced cardiomyocyte derived from a healthy human.

This invention discloses a gas shear-based microfluidic droplet preparation device for cell culture, belonging to the fields of biomedical engineering and cell culture technology. The device includes a microfluidic chip body fixed on a substrate, a sample inflow module, a gas inflow module, a droplet generation module composed of coaxially arranged sample and gas channels, and a biocompatible solidification and collection pool. This invention utilizes gas shear to avoid the toxicity of organic solvents, and the collection pool contains a mixture of culture medium and solidifying agent, achieving integrated droplet generation, solidification, and culture, thus reducing the risk of contamination. Through channel size control, inner wall cell adhesion inhibition, and multilayer coaxial structure design, it is possible to prepare cell microsphere carriers with uniform size, controllable diameter (150-400 μm), and cell viability exceeding 93%, showing broad application prospects in three-dimensional cell culture, tissue engineering, and regenerative medicine.