Method for producing a cell culture substrate

Abstract

A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.

Description

BACKGROUND OF THE INVENTION [0001] 1. Field of the Invention [0002] The present invention relates to a method for producing a cell culture substrate on which cells are caused to adhere in a highly precise pattern form. [0003] 2. Description of the Related Art [0004] At present, cell cultures of various animals and plants are performed, and also new cell culture methods are in development. The technologies of the cell culture are utilized, such as, to elucidate the biochemical phenomena and natures of cells and to produce useful substances. Furthermore, with cultured cells, an attempt to investigate the physiological activity and toxicity of artificially synthesized medicals is under way. [0005] Some cells, particularly a lot of animal cells have the adhesion dependency of adhering to some materials and growing thereon, and cannot survive for a long period under a flotation condition out of organisms. For culturing cells having such adhesion dependency, a carrier to which cells can a...

AI Technical Summary

Benefits of technology

The present invention provides a new method for producing a cell culture substrate that allows for precise placement of cells onto a base material. This method involves creating a patterned layer on the base material using a photocatalyst and energy irradiation, followed by applying a cell-containing liquid to the patterned layer using a region-selecting applying method. The invention has several advantages, including a decrease in cell loss, the ability to easily obtain various types of cells, and the ability to pattern cells in a highly precise manner. The method can be performed using a patterning substrate with a photocatalyst-containing cell adhesive layer or a photocatalyst-containing cell adhesion-inhibiting material layer, and the energy irradiating process can be from either a given direction or in a patterned form. The invention provides a more efficient and effective method for producing cell culture substrates for various applications.

Problems solved by technology

Therefore, loss is generated out of the used cells.

For this reason, it is difficult to apply such a patterning method to the case of the kind of cells which cannot be easily obtained.

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