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Method for producing a cell culture substrate

a cell culture substrate and substrate technology, applied in the field of cell culture substrate production, can solve the problems of difficult application of patterning methods to the case of cells which cannot be easily obtained, loss generated out of used cells, etc., and achieve the effect of high-quality cell culture substrates, and preventing the extinction or damage of cells

Inactive Publication Date: 2007-10-18
DAI NIPPON PRINTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0298] The present invention is not limited to the above-mentioned embodiments. The embodiments are mere examples, and all techniques having substantially the same technical concept and producing substantially the same advantageous effects as the techniques recited in the claims of the invention, and all equivalents thereof are included in the technical scope of the invention.
[0299] Hereinafter, examples are shown and thereby the present invention will be more specifically described.

Problems solved by technology

Therefore, loss is generated out of the used cells.
For this reason, it is difficult to apply such a patterning method to the case of the kind of cells which cannot be easily obtained.

Method used

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  • Method for producing a cell culture substrate
  • Method for producing a cell culture substrate
  • Method for producing a cell culture substrate

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third embodiment

(3) Third Embodiment

[0185] The third embodiment of the present process is performed when the patterning substrate is the third embodiment, and is a process of irradiating energy from a given direction, thereby forming a pattern composed of a cell adhesion-inhibiting portion where the cell adhesive material comprised in the cell adhesive layer is decomposed or denatured, and a cell adhesion portion which is other than the cell adhesion-inhibiting portion.

[0186] In the embodiment, energy is irradiated in a pattern form for forming the cell adhesion-inhibiting portion, thereby exciting the photocatalyst in the photocatalyst-containing layer so that the cell adhesive material can be decomposed or denatured. It is therefore possible to render the region irradiated with energy the cell adhesion-inhibiting portion, where the cell adhesive material is decomposed or denatured, and render the region not irradiated with the energy the cell adhesion portion, where the cell adhesive material re...

fourth embodiment

(4) Fourth Embodiment

[0188] The fourth embodiment of the present process is performed when the patterning substrate is the fourth embodiment, and is a process of irradiating energy from a given direction, thereby forming a pattern composed of a cell adhesion portion where the cell adhesion-inhibiting material comprised in the cell adhesion-inhibiting material layer is decomposed or denatured and a cell adhesion-inhibiting portion which is other than the cell adhesion portion.

[0189] In the embodiment, energy is irradiated in a pattern form for forming the cell adhesion portion, thereby exciting the photocatalyst in the photocatalyst-containing layer so that the cell adhesion-inhibiting material can be decomposed or denatured. It is therefore possible to render the region irradiated with energy the cell adhesion portion, where the cell adhesion-inhibiting material is decomposed or denatured, and render the region not irradiated with the energy the cell adhesion portion, where the cel...

fifth embodiment

(5) Fifth Embodiment

[0192] The fifth embodiment of the present process is performed when the patterning substrate is the fifth embodiment, and is a process of arranging the cell adhesive layer and a photocatalyst-containing layer side substrate having a base body and a photocatalyst-containing layer comprising a photocatalyst to dispose the cell adhesive layer and the photocatalyst-containing layer facing each other, and then irradiating energy onto the resultant from a given direction, thereby forming a pattern composed of a cell adhesion-inhibiting portion where the cell adhesive material comprised in the cell adhesive layer is decomposed or denatured, and a cell adhesion portion which is other than the cell adhesion-inhibiting portion.

[0193] As shown in, for example, FIGS. 2A and 2B, in the energy irradiating process of the embodiment, a cell adhesive layer 12 formed on a base material 1 and a photocatalyst-containing layer side substrate 23, having a photocatalyst-containing la...

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Abstract

A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.

Description

BACKGROUND OF THE INVENTION [0001] 1. Field of the Invention [0002] The present invention relates to a method for producing a cell culture substrate on which cells are caused to adhere in a highly precise pattern form. [0003] 2. Description of the Related Art [0004] At present, cell cultures of various animals and plants are performed, and also new cell culture methods are in development. The technologies of the cell culture are utilized, such as, to elucidate the biochemical phenomena and natures of cells and to produce useful substances. Furthermore, with cultured cells, an attempt to investigate the physiological activity and toxicity of artificially synthesized medicals is under way. [0005] Some cells, particularly a lot of animal cells have the adhesion dependency of adhering to some materials and growing thereon, and cannot survive for a long period under a flotation condition out of organisms. For culturing cells having such adhesion dependency, a carrier to which cells can a...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/02B41J2/175B01L3/00B01L3/02B41J2/01B41J2/06C12N5/00
CPCB01L3/0268B01L3/5085B01L2200/12B01L2300/069B01L2300/10C12M25/06B01L2400/0439C12N5/0068C12N2533/12C12N2535/10B01L2300/1888C12M23/20
Inventor MIYAKE, HIDEYUKIHATTORI, HIDESHITAKAHASHI, YOICHI
Owner DAI NIPPON PRINTING CO LTD
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