Self-assembling peptide surfaces for cell patterning and interactions

a peptide surface and cell patterning technology, applied in the field of self-assembling peptide surfaces for cell patterning and interactions, can solve the problems of sams, can be difficult and/or expensive to manufacture, and achieve the effect of convenient manufacture and purification

Inactive Publication Date: 2006-01-26
USNRKOCKUMS CANCAR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0005] This invention is based upon the discovery that improved SAMs can be manufactured by imprinting reactive self assembling peptides onto solid supports. The SAMs are characterized by ease of manufacture and purification. They are versatile in their ability to readily provide a large variety of chemical reactive moieties, or “presenting groups”, to selected targets. For example, the SAM's of the present invention can be readily designed to present ligands to cellular receptors, cell adhesion motifs, antibodies or antigen-binding fragments thereof to cell surface proteins. This preferred class of SAMs can be used to bind a target, e.g. a selected cell or cells, to a predetermined locus on the solid support.

Problems solved by technology

However, these molecules, and the resulting SAMs, can be difficult and / or expensive to manufacture.

Method used

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  • Self-assembling peptide surfaces for cell patterning and interactions
  • Self-assembling peptide surfaces for cell patterning and interactions
  • Self-assembling peptide surfaces for cell patterning and interactions

Examples

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example 1

Preparation of Patterned SAMs Glass Chip

[0069] A 10:1 (w:w) mixture of Sylgard Silicone Elastomer 184 and Sylgard Curing Agent 184 (Dow Corning Corp., Midland, Mich.) was casted over a master, which was generated by photolithography, and pressure degassed. After sitting at room temperature for 1 hour, the PDMS was cured at 60° C. for 2 hours. The stamp was carefully peeled off the master after cooling to room temperature and rinsed with ethanol. The PDMS stamp was inked by a cotton swab which has been moistened with a 5 mM solution of (1-mercaptoundec-11-yl)hexa(ethylene glycol) (HO(CH2CH2O)6(CH2)11SH) in ethanol. The resulting stamp was placed on the gold substrate (125 ★ gold on a titanium-primed 24×50-2 microscope cover glass) and gentle hand pressure was applied to aid in complete contact between the stamp and the glass chip. After 1 minute, the stamp was peeled off the glass chip and the resulting substrate was immersed directly in a 2 mM solution of (RADC)3 AAAC (SEQ ID NO: 1...

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Abstract

This invention describes self assembled monolayers (SAMs) manufactured by imprinting reactive peptides onto solid supports. The invention further relates to methods of preparing and using these improved SAMs.

Description

RELATED APPLICATIONS [0001] This application is a continuation of U.S. application Ser. No. 10 / 317,838, filed Dec. 11, 2002, which is a continuation of U.S. application Ser. No. 10 / 071,500, filed Feb. 8, 2002, which is a continuation of U.S. application Ser. No. 08 / 882,415, filed Jun. 25, 1997. The entire teachings of the above applications are incorporated herein by reference.GOVERNMENT SUPPORT [0002] The invention was supported, in whole or in part, by a grant (Grant No. DAAH04-94-G-0407) from the Army Research Office. The Government has certain rights in the invention.BACKGROUND OF THE INVENTION [0003] Organic surfaces have been employed in numerous methods and systems, including as substrates for ELISA, cell and tissue culture. Self-assembled monolayers (SAMs) are a class of organic surfaces manufactured by imprinting a monolayer of organic compounds with reactive moieties onto a solid support under conditions wherein the compounds react with and bind to the solid support in a s...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C40B20/02C12M1/34B01J19/00C07K2/00C07K7/08C07K17/14C12N5/00C40B40/10C40B60/14G03F7/00G03F7/16
CPCB01J19/0046G01N2610/00B01J2219/00605B01J2219/00612B01J2219/00617B01J2219/00619B01J2219/00626B01J2219/0063B01J2219/00635B01J2219/00637B01J2219/00659B01J2219/00725B01J2219/00743B82Y5/00B82Y10/00B82Y30/00B82Y40/00C07K2/00C07K7/08C07K17/14C12N5/0068C12N2535/10C40B40/10C40B60/14G01N33/54353G03F7/0002G03F7/165B01J2219/00382
Inventor ZHANG, SHUGUANGRICH, ALEXANDERYAN, LINWHITESIDES, GEORGE
Owner USNRKOCKUMS CANCAR
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