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Application of tannic acid serving as micro-contact printing ink to cell patterning

A technology of micro-contact printing and cell patterning, applied in the field of cell culture, can solve problems such as time-consuming and opaque patterns, and achieve the effects of simple steps, low price and wide sources.

Inactive Publication Date: 2018-12-25
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In response to the above limitations, Jiang Xingyu's research group at the National Center for Nanoscience and Technology developed a polydopamine-based one-step printing method for cell patterning, which is suitable for a variety of substrates, such as gold surfaces, glass, silicon surfaces, polystyrene (PS) surface, etc., have the characteristics of easy operation and low cost, but also have the disadvantages of opaque patterns and long time-consuming

Method used

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  • Application of tannic acid serving as micro-contact printing ink to cell patterning

Examples

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Effect test

Embodiment 1

[0037] Printing of Tannic Acid Patterns on the Surface of Polyethylene Glycol Silane Modified Quartz Chips

[0038] 1. Oxidize the quartz sheet

[0039] Quartz sheet (8*8*0.5 mm 3 , purchased from Donghai County Weida Quartz Products Co., Ltd.) in acetone and absolute ethanol for 10 minutes of ultrasonic cleaning, then boiled in a mixture of concentrated sulfuric acid and hydrogen peroxide (volume ratio 3:1) for 1 hour, and cleaned with ultrapure water. blow dry. The quartz slice was oxidized in a low-temperature plasma processor for 6 min.

[0040] 2. Inert modification of quartz slices

[0041] The oxidized quartz slice was soaked in 6 g / L methoxypolyethylene glycol silane (MW2000) in toluene solution for 24 h, and 1% triethylamine was added as a catalyst. After the reaction was completed, wash 2 times with absolute ethanol, wash 2 times with ultrapure water, N 2 Blow dry and store in N 2 atmosphere.

[0042] 3. Preparation of polydimethylsiloxane stamp

[0043] Firs...

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Abstract

The invention provides an application of tannic acid serving as micro-contact printing ink to cell patterning. According to the application, firstly, a substrate is subjected to polyethylene glycol surface treatment to obtain an 'inert' substrate; secondly, water solution of tannic acid is prepared, and a seal is prepared; thirdly, the seal is soaked into the water solution of the tannic acid; finally, the seal adsorbing the tannic acid is covered on the 'inert' substrate, so that a pattern on the seal is acquired on the surface of the substrate. The pattern is transparent and can chelate ferroferric oxide nano-particles, so that the pattern is visual, the patterned substrate can further be used for patterned growth of cells, a patterned method is simple to operate, and an existing cell patterning technique is simplified.

Description

technical field [0001] The invention relates to the technical field of cell culture, and more specifically relates to the application of tannic acid as microcontact printing ink in cell patterning. Background technique [0002] Cell patterning is an in vitro cell culture technology. Specifically, by preparing a certain patterned substrate, the cell's own response mechanism is induced to adhere to the designed area to form a pattern. This method is different from the direct seeding of cells into culture dishes commonly used in the laboratory, which usually forms a cell monolayer. [0003] Cell patterning technology has become an important tool to study and control cell biological behavior, and is widely used to study cell-cell, cell-substrate interactions (including cell adhesion and apoptosis, cell polarization behavior, Cell migration rate, cell differentiation behavior, etc.); in vitro models can also be constructed for use in tissue engineering, cell sensing, drug screen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09D11/03C12N5/00
CPCC09D11/03C12N5/0068C12N2533/12C12N2533/30
Inventor 孙康杨明亮张超蒋庆
Owner SUN YAT SEN UNIV
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