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High-accuracy biological 3D (three-dimensional) printing method

A 3D printing, high-precision technology, applied in medical science, prosthesis, additive processing, etc., can solve problems such as cost manufacturing

Active Publication Date: 2015-09-09
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have made great progress in terms of cell viability and multi-cell printing, but the main challenge is that it is impossible to produce a microstructural environment close to the scale of cells and extracellular matrix at a low cost, so as to precisely regulate cell differentiation. Growth and Tissue Regeneration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A high-precision biological 3D printing method, comprising the following steps:

[0020] 1) According to the cell pattern to be printed or the three-dimensional cell gel structure, a program to control the movement of the platform is programmed on the computer, and 40 μm is selected as the line width of the pattern to be printed, so that the moving speed of the platform is determined to be 60mm / s;

[0021] 2) Configure a mixed solution of cells / growth factors / biological materials for 3D printing. The biological materials used are sodium alginate and PVA, and their mass concentrations are 0.5% and 6% respectively; the cells used are fibroblasts , the concentration of cells in the mixed solution is 2×10 6 each / ml, the concentration of growth factor in the mixed solution is 1 μg / ml;

[0022] 3) Inhale the prepared mixed solution of cells / growth factors / biological materials into a syringe equipped with a metal nozzle. The inner diameter of the metal nozzle is 500 μm, and t...

Embodiment 2

[0028] A high-precision biological 3D printing method, comprising the following steps:

[0029] 1) According to the cell pattern to be printed or the three-dimensional cell gel structure, a program to control the movement of the platform is programmed on the computer, and 150 μm is selected as the line width of the pattern to be printed, so that the moving speed of the platform is determined to be 5mm / s;

[0030] 2) The biomaterials used to configure the mixed solution of cells / growth factors / biomaterials for 3D printing are PVA and type II rat tail collagen, and their mass concentrations are 8% and 0.5% respectively; the cells used are blood vessels Endothelial cells, the concentration of cells in the mixed solution is 1×10 7 individual / ml, the concentration of growth factors in the mixed solution is 0.05 μg / ml;

[0031] 3) Inhale the configured cells / growth factors / biological materials into a syringe equipped with a metal nozzle. Connect to the positive pole of the DC high...

Embodiment 3

[0037] A high-precision biological 3D printing method, comprising the following steps:

[0038] 1) According to the cell pattern to be printed or the three-dimensional cell gel structure, a program to control the movement of the platform is programmed on the computer, and 20 μm is selected as the line width of the pattern to be printed, so that the moving speed of the platform is determined to be 500mm / s;

[0039]2) Configure a mixed solution of cells / growth factors / biological materials for 3D printing. The biological materials used are gelatin solutions with a mass concentration of 5% respectively; the cells used are stem cells, and the cells in the mixed solution The concentration is 1×10 5 per ml, the concentration of growth factors in the mixed solution is 0.5 μg / ml;

[0040] 3) Inhale the prepared mixed solution of cells / growth factors / biological materials into a syringe equipped with a metal nozzle. The inner diameter of the metal nozzle is 1000 μm, and the syringe is c...

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Abstract

Disclosed is a high-accuracy biological 3D (three-dimensional) printing method. According to the method, firstly, procedures for controlling a platform to move are compiled on a computer according to cell patterns wanting to be printed or three-dimensional cell gel structures, secondly, mixed solution of cells / growth factors / biological materials for 3D printing is selected, the cells / the growth factors / the biological materials are sucked into a syringe with a metal sprayer in a mixed manner, the metal sprayer is connected with a positive electrode of direct-current high-voltage power supply, a receiving plate is fixed on the movable platform below the metal sprayer and connected with the ground, the mixed solution forms material jet streams by the aid of electrostatic force, and the movable procedures on the movable platform are started, so that the material jet streams form the predesigned patterns or structures on the receiving plate. According to a cross linking mode, a treatment mode of temperature and calcium ions is changed, so that the material jet streams rapidly forms cells / hydrogel in situ through illumination. Various fine cell patterns and three-dimensional cells / gel micro-structural systems can be rapidly printed on a substrate as required.

Description

technical field [0001] The invention relates to the technical field of cell printing, in particular to a high-precision biological 3D printing method. Background technique [0002] Boland et al. proposed the concept of "cell printing" technology in 2003. This technology uses the principles and methods of traditional additive manufacturing, and uses biological materials, growth factors and active cells as materials to carry out controllable manufacturing of complex structures such as artificial tissues and organs. In the process of "cell printing", the mixture of cells (or cell aggregates), sol (precursor of hydrogel) and growth factors is placed in the nozzle of the printer at the same time, and the deposition position of the droplet containing cells is controlled by the computer , complete the construction of a three-dimensional multicellular hydrogel organoid structure layer by layer by going from point to line, from line to surface, and from surface to body. Compared wit...

Claims

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Application Information

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IPC IPC(8): A61F2/02B33Y10/00B33Y40/00B33Y50/02B33Y30/00
Inventor 贺健康赵翔李涤尘刘亚雄靳忠民
Owner XI AN JIAOTONG UNIV
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