Tryptophan tension chain-beta-hairpin antibacterial peptide and preparation method and application thereof

A tryptophan zipper and antibacterial peptide technology, applied in the biological field, can solve the problems of increasing synthesis difficulty, production cost, loss, biological activity decline, etc.
CN106749531AInactive Publication Date: 2017-05-31NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Publication Date
2017-05-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a tryptophan tension chain-beta-hairpin antibacterial peptide and a preparation method and application thereof. A sequence of the antibacterial peptide is as shown in a sequence table SEQ ID No.1. The preparation method comprises the following steps: by taking a tryptophan tension chain structure as a template, combining amino acid composition and structural characteristics of the antibacterial peptide; and by using interaction of cross-chain W-W as a structural stable factor, symmetrically and circularly arranging an amino acid lysine with charges and a hydrophobic amino acid tryptophan as repeated binary sequence units on two arms of the beta-hairpin to obtain the antibacterial peptide WK3. The antibacterial peptide has relatively high bacteriostatic activity and cell selectivity, and the treatment index reaches up to 161.27. The antibacterial peptide designed by the method needs not to bind a disulfide bond but has extremely high structural stability, so that the selectivity of the antibacterial peptide between bacterial cells and mammalian cells is improved, and the antibacterial peptide has an application potential as an antibiotic substituent. The antibacterial peptide is relatively high in activity and relatively low in cytotoxicity.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a tryptophan zipper β-hairpin antibacterial peptide and its preparation method and application. Background technique

[0002] The rapid development of the feed industry is inseparable from the important role and contribution of antibiotic feed additives. However, the long-term overuse or even abuse of antibiotics has led to the widespread emergence of drug residues and drug-resistant strains around the world, posing a direct threat to public health and environmental safety. Antimicrobial peptides have the characteristics of high antibacterial activity, no residue, and easy production, and their antibacterial mechanism is different from traditional antibiotics, making bacteria less resistant to antibiotics, so they have the potential to be developed into a new generation of antibacterial drugs. Among them, β-hairpin structure antimicrobial peptides have received widesprea...

Claims

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