Antibacterial peptide RW-P and preparation method and application thereof

Abstract

The invention provides an antibacterial peptide RW-P and a preparation method and an application thereof. The sequence of the antibacterial peptide RW-P is as shown in SEQ ID No.2. The preparation method comprises the following steps: by taking two simple amino acids as basic amino acids, repeating the amino acids in a sequence form of XXYY for three times to obtain alpha-spiral antibacterial peptide RW, wherein X is arginine and Y is tryptophan; and performing amino acid residue substitution at fixed sites on arginine at the 5th site and tryptophan at the 8th site of the RW antibacterial peptide, and introducing proline to obtain the antibacterial peptide RW-P. Computer structure prediction and circular dichroism spectrum structure determination verify that RW is of an alpha-spiral structure, and the spiral content of RW-P is reduced by introducing proline. Researches find that RW-P keeps a high antibacterial activity of RW, and meanwhile, the cytotoxicity is reduced and the cell selectivity is improved. The method provides a theoretical foundation for designing the efficient low-degree antibacterial peptide which is applied to animal production.

Description

technical field [0001] The invention belongs to the field of agriculture, animal husbandry and veterinary medicine, and provides an antibacterial peptide RW-P, a preparation method and application thereof. Background technique [0002] Antibiotics have been used as feed additives in animal production for more than half a century, but with the extensive use of antibiotics, especially the abuse, the problems in Britain, France and Germany have become more and more serious. The problem of bacterial antibiotic resistance and the residue of antibiotics in animal products have seriously affected the development of animal husbandry and threatened human health. Therefore, it is an urgent problem to be solved in the development of modern animal husbandry to find new green antibiotic substitutes as new feed additives for animal production. [0003] Antimicrobial peptides (AMPs) are a class of small molecular polypeptides that exist in the immune system of organisms, and are the first...

AI Technical Summary

Problems solved by technology

At present, many studies on antimicrobial peptides focus on the transformation of natural antimicrobial peptides, but the antimicrobial peptide derivatives obtained by this method usually do not have good antibacterial activity. The sequence is similar, its stability in the body is not good, it is easily metabolized by the enzyme in the body, and loses its activity

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