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Method for promoting regeneration of camellia oleifera plants with buds and stems through light regulation and control

A technology of light regulation and stem segments, which is applied in plant regeneration, botany equipment and methods, gardening methods, etc., can solve the problems of high multiplication coefficient and scarcity of Camellia oleifera seedlings, achieve high multiplication rate, reduce bacterial infection rate, The effect of high germination rate

Pending Publication Date: 2022-04-15
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Problems solved by technology

[0004] The object of the present invention is to provide a method for light regulation to promote the regeneration of Camellia oleifera bud stem plants, solve the technical problem of scarcity of Camellia oleifera seedlings, and obtain the best culture conditions with high axillary bud induction rate, high multiplication coefficient and high emergence rate

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  • Method for promoting regeneration of camellia oleifera plants with buds and stems through light regulation and control
  • Method for promoting regeneration of camellia oleifera plants with buds and stems through light regulation and control
  • Method for promoting regeneration of camellia oleifera plants with buds and stems through light regulation and control

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[0024] Preferred embodiments of the present invention are provided below to facilitate further understanding of the present invention. Those skilled in the art should understand that the descriptions of the embodiments of the present invention are only exemplary, and are not intended to limit the solutions of the present invention.

[0025] In the present invention, the research and development of the method for promoting the regeneration of Camellia oleifera with bud stems by light regulation includes the following contents:

[0026] S1. Primary culture of stem segments with buds

[0027] On sunny days from April to May, the Camellia oleifera variety 'Huajin' from the Camellia oleifera cultivar (28°33′N; 112°35′E) of the Camellia oleifera harvesting garden (28°33′N; 112°35′E) in Dongcheng Town, Wangcheng County, Changsha City, Hunan Province was selected Semi-lignified stem segments with axillary buds are used as materials. Cut off the leaves of the collected stem sections ...

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Abstract

The method for promoting regeneration of camellia oleifera plants with buds and stems through light regulation comprises the steps that S1, disinfected stems with buds and stems are inoculated to an induction culture medium and placed under white light, and the induction culture medium is 1 / 2 MS + 1.0 mg.L <-16 >-BA + 0.01 mg.L <-1 > NAA; s2, transferring the axillary buds into a proliferation culture medium, and placing the axillary buds in a proliferation culture medium MS + 1.0-4.0 mg.L <-16 >-BA + 0.01-0.2 mg.L <-1 > IBA, wherein the ratio of red to blue is 4: 1; s3, transferring the axillary buds to a strong seedling culture medium, and culturing the axillary buds under the condition that the ratio of red to blue is 4: 1, wherein the strong seedling culture medium is 1 / 2MS, 5.0 mg.L <-1 > GA and 0.01 mg.L <-1 > IAA; s4, transferring the buds to a rooting culture medium, and placing the buds in a rooting culture medium of 1 / 2 MS, wherein the ratio of red to blue is 4: 1; s5, acclimatization and transplantation. The method has the effects of high axillary bud induction rate, high proliferation rate and high emergence rate.

Description

technical field [0001] The invention belongs to the technical field of Tung aleuca tissue culture, and in particular relates to a method for promoting the regeneration of Camellia oleifera with buds and stem segments by light regulation. Background technique [0002] Camellia oleifera is native to my country and is mainly distributed in Hunan, Guangxi, Zhejiang, Jiangxi and other regions of China. It is an evergreen shrub or small tree of the camellia family. It is an important woody oil plant in China. , olive and coconut are also known as "the world's four major woody oils". Its main product, camellia oil, is of high quality and rich in unsaturated fatty acids, squalene, tocopherols and other ingredients that are beneficial to human health. In recent years, my country has vigorously promoted the Camellia oleifera industry. According to statistics, the planting area of ​​Camellia oleifera in my country has reached 67 million mu, and the total output value of the Camellia ole...

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/28A01G24/15
Inventor 曾艳玲王肖何超银盘鑫海葛璐瑶赫雯琳杜勤辉
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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