A method for tissue culture, rapid propagation and cultivation of P.

A technology of tissue culture rapid propagation and Paphiopedilum, applied in flower cultivation, horticultural methods, botanical equipment and methods, etc., can solve problems such as difficulty in meeting industrial production, low germination rate, and small seeds, and achieve easy mastery and investment The effect of low cost and simple operation

Inactive Publication Date: 2020-05-01
四川省自然资源科学研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Like most orchids, Paphiopedilum hengduan has no endosperm and small seeds. In nature, it can only germinate and grow with the nutrition provided by symbiotic fungi. It is difficult to reproduce under natural conditions, and the germination rate is extremely low.
The traditional propagation method of Paphiopedilum is branch propagation, which has a low reproduction coefficient and slow speed, and it is difficult to meet the requirements of industrial production. Rapid propagation by tissue culture is an important means of protection and development.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0079] (1) Address: The tissue culture and rapid propagation of capsule seeds is Chengdu Shuangliu Base of Hengduanshan Biotechnology Co., Ltd. The greenhouse planting address is Hengduanshan Huanglong Base at an altitude of 3,200 meters and Chengdu Shuangliu Base of Hengduanshan Biotechnology Co., Ltd. at an altitude of 498 meters.

[0080] (2) Time: The start time of capsule tissue culture is from September 1, 2010 to November 2010, the transplanting time is from May 1, 2012 to September 2015, and the flowering observation time is from February 2014 to October 2016 moon.

[0081] (3) Materials: Collected from mature capsules of Paphiopedilum Yazhi that matured naturally or after artificial pollination (300 days).

[0082] details as follows:

[0083] (1) After washing the mature capsules of Yazhi Paphiopedilum with distilled water, first disinfect them with 10% sodium hypochlorite (prepared for current use) on an ultra-clean workbench for 20 minutes, then use 70% alcohol fo...

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PUM

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Abstract

The invention discloses a method for tissue culture, rapid propagation and cultivation of P. chinensis P. hydrangea. Capsule collection and preservation → disinfection → induction of protocorms → proliferation and differentiation cultivation → strong seedling and rooting cultivation → seedling hardening and transplanting → transplanting Greenhouse planting → pest control and other steps to breed seedlings efficiently and quickly. The tissue culture rapid propagation technology system established by the present invention does not need to invest too much infrastructure in the whole process. A capsule that has been pollinated successfully for 300 days can obtain 2000-3000 complete plants after 18 months. Using a specific medium, the seeds germinate The rate is more than 80%, the input cost is low, the operation is simple, easy to master, and the success rate is high. It has realized the artificial mass breeding of Hengduan Yazhi Paphiopedilum, which is of great importance to the regeneration and sustainable utilization of endangered plant resources and the protection of germplasm resources. meaning.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for tissue culture rapid propagation and cultivation of P. Background technique [0002] Paphiopedilum ( Paphiopedilum ) is also known as slipper orchid, cinderella, etc. There are about 88 species of species discovered in the world at present, and there are 28 species in my country. Due to its unique flower shape, gorgeous flower color, long flowering period and high ornamental value, Paphiopedilum is a very popular high-grade flower in the international flower market. In recent years, due to the increase of human activities, habitat fragmentation, over-harvesting and grazing, the wild populations and individual numbers of P. Accession to the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Hengduan elegant Paphiopedilum ( Paphiopedilum Hengduan Grace Helen) is a hybrid species registered in the Royal Horticultural Society (R...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/63
CPCA01H4/005A01H4/008
Inventor 鲁松豪格尔帕奈文清帕奈周蓉干永进向丽谷海燕余道平吴霞
Owner 四川省自然资源科学研究院
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