Artificial quick propagation method for hemsley rockvine root

Abstract

The invention relates to a method of artificial fast breeding for spun gold suspended gourd, with the detail steps in below: 1) pretreating the explant, 2) sterilizing the explant, 3) inoculating, 4) inducing culturing, 5) differentiation culture for callus, 6) enrichment culture, 7) strengthening sprout culture, 8) root-growing culture, 9) sprouting training and replanting, 10) sprout bed management, 11) bedding in earth the culture sprout, 12) shearing the cuttage ear, 13) treating the cuttage ear with medical agent, 14) cuttage, 15) cuttage sprout bed management. The benefit of this invention is that:: by making use of the advantage of plant tissue culture technique to breed large quantity of spun gold suspended gourd tissue culture sprout in tube with a high speed, the monthly breeding rate is 3-5 times, the yearly tissue culture sprout producing ability is more than ten thousand per square meter, and it solves the problem of spun gold suspended gourd resource shortage. The provided artificial fast breeding method uses less explant material, which can effectively save rare endangered plant species, and the mass production can be carried out which can provide seed foundation for the further development of spun gold suspended gourd product.

Description

technical field

[0001] The invention relates to a combination technology of plant tissue culture propagation and plant non-test tube propagation (artificial rapid propagation technology of wild medicinal plants), mainly an artificial rapid propagation method of golden silk gourd. Background technique

[0002] Tetrastlgma hemsleyanum Diels et Gilg, also known as Tetrastlgma hemsleyanum Diels et Gilg, is a perennial vine of the family Vitis vinifera. It has the functions of clearing heat and spasm, dispelling wind and phlegm, promoting blood circulation and relieving pain. It is clinically used to treat children with febrile convulsions, abdominal pain, pneumonia, asthma, hepatitis and other diseases. It has been proved by modern medical research that its active ingredients are flavonoids, which can act on topoisomerase, inhibit the expansion of tumors, and are used for the treatment of various malignant tumors. Because the golden silk hanging gourd has very peculiar requirem...

Examples

Embodiment 1

[0050] 1) Pretreatment of explants: cutting rattans with a length of 10-15 cm, rinsing with tap water with 1% detergent by volume, and then rinsing with tap water for about 30 minutes.

[0051] 2) Sterilization of explants: sterilize the pretreated materials on an ultra-clean workbench, soak them in alcohol with a volume ratio of 75% for 0.5-1.0 min, rinse them once with sterile water, and then Sterilize with 0.1% mercuric chloride solution by weight for 10-15 minutes, or sterilize with 2% sodium hypochlorite aqueous solution for 10-15 minutes by volume, and finally rinse with sterile water for 3-5 times.

[0052] 3) Inoculation: put the sterilized material on the sterilized filter paper to blot dry, cut out terminal buds and stem segments with axillary buds about 0.5-1.0 cm long with a scalpel, and inoculate them in the induction medium.

[0053] 4) Induction culture: Induction culture until terminal buds and axillary buds are induced, callus occurs at the base of the stem se...

Embodiment 2

[0068] 1) Explant pretreatment: cut rattans with a length of 10-15 cm, rinse with tap water with 1% detergent, and rinse with tap water for about 30 minutes.

[0069] 2) Sterilization of explants: sterilize the pretreated materials on an ultra-clean workbench, first soak in 75% alcohol for 0.5-1.0min, rinse once with sterile water, and then use 0.1% Sterilize with mercuric chloride solution for 10 to 15 minutes, or with 2% sodium hypochlorite aqueous solution for 10 to 15 minutes, and finally rinse with sterile water for 3 to 5 times.

[0070] 3) Inoculation: put the sterilized material on the sterilized filter paper to blot dry, cut out terminal buds and stem segments with axillary buds about 0.5-1.0 cm long with a scalpel, and inoculate them in the induction medium.

[0071] 4) Induction culture: Inoculate the terminal buds and stem segments with axillary buds in the bud induction medium of MS+6-BA 2mg / L+IBA0.2mg / L+sugar 30g / L+agar 7g / L. After 10 days of induction culture, ...

technical field

[0001] The invention relates to a combination technology of plant tissue culture propagation and plant non-test tube propagation (artificial rapid propagation technology of wild medicinal plants), mainly an artificial rapid propagation method of golden silk gourd. Background technique

[0002] Tetrastlgma hemsleyanum Diels et Gilg, also known as Tetrastlgma hemsleyanum Diels et Gilg, is a perennial vine of the family Vitis vinifera. It has the functions of clearing heat and spasm, dispelling wind and phlegm, promoting blood circulation and relieving pain. It is clinically used to treat children with febrile convulsions, abdominal pain, pneumonia, asthma, hepatitis and other diseases. It has been proved by modern medical research that its active ingredients are flavonoids, which can act on topoisomerase, inhibit the expansion of tumors, and are used for the treatment of various malignant tumors. Because the golden silk hanging gourd has very peculiar requirem...

Examples

Embodiment 1

[0050] 1) Pretreatment of explants: cutting rattans with a length of 10-15 cm, rinsing with tap water with 1% detergent by volume, and then rinsing with tap water for about 30 minutes.

[0051] 2) Sterilization of explants: sterilize the pretreated materials on an ultra-clean workbench, soak them in alcohol with a volume ratio of 75% for 0.5-1.0 min, rinse them once with sterile water, and then Sterilize with 0.1% mercuric chloride solution by weight for 10-15 minutes, or sterilize with 2% sodium hypochlorite aqueous solution for 10-15 minutes by volume, and finally rinse with sterile water for 3-5 times.

[0052] 3) Inoculation: put the sterilized material on the sterilized filter paper to blot dry, cut out terminal buds and stem segments with axillary buds about 0.5-1.0 cm long with a scalpel, and inoculate them in the induction medium.

[0053] 4) Induction culture: Induction culture until terminal buds and axillary buds are induced, callus occurs at the base of the stem se...

Embodiment 2

[0068] 1) Explant pretreatment: cut rattans with a length of 10-15 cm, rinse with tap water with 1% detergent, and rinse with tap water for about 30 minutes.

[0069] 2) Sterilization of explants: sterilize the pretreated materials on an ultra-clean workbench, first soak in 75% alcohol for 0.5-1.0min, rinse once with sterile water, and then use 0.1% Sterilize with mercuric chloride solution for 10 to 15 minutes, or with 2% sodium hypochlorite aqueous solution for 10 to 15 minutes, and finally rinse with sterile water for 3 to 5 times.

[0070] 3) Inoculation: put the sterilized material on the sterilized filter paper to blot dry, cut out terminal buds and stem segments with axillary buds about 0.5-1.0 cm long with a scalpel, and inoculate them in the induction medium.

[0071] 4) Induction culture: Inoculate the terminal buds and stem segments with axillary buds in the bud induction medium of MS+6-BA 2mg / L+IBA0.2mg / L+sugar 30g / L+agar 7g / L. After 10 days of induction culture, ...