30 results about "Depside" patented technology

Herbicidal compositions are disclosed comprising an effective amount of flufenacet, pendimethalin and diflufenican. A method of controlling harmful plants is disclosed. The compositions may be used flexibly, e.g., in post-emergent and / or pre-emergent applications. The compositions may be used prior to sowing a crop. The compositions may provide enhanced compatibility with crops. The compositions may be used on different soil types, e.g., with high-organic matter soils and / or at different soil depths, e.g., at shallow soil depths. The compositions may be used against herbicide-resistant plant species, for example EMR and TSR-resistant species and / or may be used for resistance management. The compositions may be used with various irrigation techniques.

This invention provides the process of enzymatic enhanced extraction of depsides from salvia miltiorrhiza substance. This process includes comminution, soaking, the preparation of enzyme blends, the blending of material and liquid, extraction, filtering, condensing and dring. This process makes full use of effective wall breaking and improve dissolution principle of enzyme blends, and produces water-soluble extraction powder of salvia miltiorrhiza that with many depsides functional substance. This invention integrates the enzymatic hydrolysis and extraction process, and has many benefits such as effective, mild, saving and environment friendly. The extraction rate of depsides from salvia miltiorrhiza is increased significently, reaches 9.5% that increased 3.5% compared with traditional water extraction. The temperature reduced from 100DEG C to 50DEG C, and time for extraction reduced for 1 hour. At the same time it avoids the Polyose Material Gelatinization and oxidation of Depsides such as danshensu. This process is innocuity and harmless. The products can be used as functional substance or medicine.

The invention relates to a method of artificial fast breeding for spun gold suspended gourd, with the detail steps in below: 1) pretreating the explant, 2) sterilizing the explant, 3) inoculating, 4) inducing culturing, 5) differentiation culture for callus, 6) enrichment culture, 7) strengthening sprout culture, 8) root-growing culture, 9) sprouting training and replanting, 10) sprout bed management, 11) bedding in earth the culture sprout, 12) shearing the cuttage ear, 13) treating the cuttage ear with medical agent, 14) cuttage, 15) cuttage sprout bed management. The benefit of this invention is that:: by making use of the advantage of plant tissue culture technique to breed large quantity of spun gold suspended gourd tissue culture sprout in tube with a high speed, the monthly breeding rate is 3-5 times, the yearly tissue culture sprout producing ability is more than ten thousand per square meter, and it solves the problem of spun gold suspended gourd resource shortage. The provided artificial fast breeding method uses less explant material, which can effectively save rare endangered plant species, and the mass production can be carried out which can provide seed foundation for the further development of spun gold suspended gourd product.

Herbicidal compositions are disclosed, comprising an effective amount of flufenacet, pendimethalin and metribuzin. A method of controlling harmful plants is disclosed. The compositions may be used flexibly, e.g, in post-emergent and / or pre-emergent applications. The compositions may be used prior to sowing a crop. The compositions may provide enhanced compatibility with crops. The compositions may be used on different soil types, e.g., with high-organic matter soils and / or at different soil depths, e.g., at shallow soil depths. The compositions may be used against herbicide-resistant plant species, for example EMR and TSR-resistant species and / or may be used for resistance management. The compositions may be used with various irrigation techniques.

In recent years, with the rapid growth of the traditional edible fungus industry all over China, the pollution and the hazard generated to the entironment and the edible fungus industry per se by a large number of obsolete fungus sticks (slags) after the primary planting of edible fungi also become bigger and bigger. The invention provides a method for rapidly fermenting and treating obsolete fungus sticks of edible fungi by utilizing sporotrichum thermophile, thereby not only realizing the comprehensive development and the deep utilization of wastes, but also reducing pollution sources to a large degree. In the invention, sporotrichum thermophile is utilized to rapidly ferment the obsolete fungus sticks and the fungus slags of the edible fungi, wherein the fermentation temperature is controlled within the range of 45-70 DEG C; fermentation lasts for 10-15 days, and compared with the traditional fermentation, the fermentation days are shortened by 8-10 days; the fermentation is complete, and harmful pests and eggs can be removed thoroughly; a variety of dedicated organic fertilizers can be prepared if the decomposition products of the fungus sticks are inoculated into other functional strains, coordination factors and the like; the weight proportion of the decomposition products is higher than 50 percent, and the decomposition products have high and stable activity, being beneficial to roots to absorb nutritive materials; the decomposition products can also serve as the compost for the secondary planting of the edible fungi and have convenient operation; common peasant households can prepare and then use the decomposition products on the spot and have low costs and are convenient and quick, thereby being easy to popularize.

Disclosed is a class of depside compounds as shown in the right general formula, wherein, R1 is equal to -CH3or-CH2CH3; R2 is equal to H, F, Cl or Br; R3 is equal to H, Cl, Br, OCH3, OCH2CH3 or NO2; R4 is equal to H, F, Cl, Br, OCH3, OCH2CH3 or CH3; R5 is equal to H or OCH3; n is equal to 0 or 1. The depside compounds of the invention can inhibit Gram-positive strains, while some of the depside compounds can inhibit Gram-negative strains, which are not reported in the previous literature. The invention also enables the selected compounds with good antibacterial activity to go through the test in inhibiting the IL-8 production amount caused by bacteria, while the result shows that one of the compounds has strong inhibition to IL-8 caused by the stimulation of escherichia coli, so the compound has the potential to join a class of non-steroidal anti-inflammatory drugs.

The biosynthesis of fungal bicyclo[2.2.2]diazaoctane indole alkaloids with a wide spectrum of biological activities have attracted increasing interest. Their intriguing mode of assembly has long been proposed to feature a non-ribosomal peptide synthetase, a presumed intramolecular Diels-Alderase, a variant number of prenyltransferases, and a series of oxidases responsible for the diverse tailoring modifications of their cyclodipeptide-based structural core. Until recently, the details of these biosynthetic pathways have remained largely unknown due to lack of information on the fungal derived biosynthetic gene clusters. Herein, we report a comparative analysis of four natural product metabolic systems of a select group of bicyclo[2.2.2]diazaoctane indole alkaloids including (+) / (−)-notoamide, paraherquamide and malbrancheamide, in which we propose an enzyme for each step in the biosynthetic pathway based on deep annotation and on-going biochemical studies.

The invention discloses stereum hirsutum fermentation liquor and sesquiterpene which are produced by fermenting stereum hirsutum liquor, as well as applications thereof, and particularly relates to a production technology process of sesquiterpene cultured and obtained by the stereum hirsutum liquor, and the hypoglycemic efficiency of sesquiterpene. Repeated tests and study show that a culture solution produced by the production technology contains sesquiterpene, a mouse with hyperglycemia takes the culture solution orally, and then blood sugar and fructosamine of the mouse can be reduced remarkably, therefore, the stereum hirsutum culture solution is confirmed to have the application for preparing healthy drinks and medicines capable of reducing the blood sugar. The culture solution is liquor with the color ranging from aurantium to tawny, and the sesquiterpene content in the culture solution is 0.4 mg / mL-4.0 mg / mL. According to the invention, not only is a new way explored for the deep development and utilization of the stereum hirsutum, but also an ideal traditional Chinese medicine is provided for controlling the blood sugar.

The invention provides application of compounds or pharmaceutically acceptable salts shown as the general formula I, and application of pharmaceutical compositions and plant extract products both containing the compounds or the pharmaceutically acceptable salts. The compounds or pharmaceutically acceptable salts of the general formula I, the pharmaceutical compositions and the plant extract products are capable of increasing the transcription of lipoprotein lipase in adipocyte in cell level, thereby improving lipid metabolism. Therefore, the compounds or pharmaceutically acceptable salts of the general formula I, the pharmaceutical compositions and the plant extract products are applicable to prepare medicines for treating II type diabetes and hypertriglyceridemia.

Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.)

This invention provides the process of enzymatic enhanced extraction of depsides from salvia miltiorrhiza substance. This process includes comminution, soaking, the preparation of enzyme blends, the blending of material and liquid, extraction, filtering, condensing and dring. This process makes full use of effective wall breaking and improve dissolution principle of enzyme blends, and produces water-soluble extraction powder of salvia miltiorrhiza that with many depsides functional substance. This invention integrates the enzymatic hydrolysis and extraction process, and has many benefits suchas effective, mild, saving and environment friendly. The extraction rate of depsides from salvia miltiorrhiza is increased significently, reaches 9.5% that increased 3.5% compared with traditional water extraction. The temperature reduced from 100DEG C to 50DEG C, and time for extraction reduced for 1 hour. At the same time it avoids the Polyose Material Gelatinization and oxidation of Depsides such as danshensu. This process is innocuity and harmless. The products can be used as functional substance or medicine.

The invention discloses a plant lactobacillus plantarum tannase gene and application thereof. The nucleotide sequence of the plant lactobacillus plantarum tannase gene Tg is shown in SEQ ID NO.1, thenucleotide sequence of a plant lactobacillus plantarum tannase mutant gene MTg is shown in SEQ ID NO.2, and the mutant gene MTg is obtained by transforming gene fragments of the plant lactobacillus plantarum tannase gene Tg by using a fixed point mutation technology. By adopting the plant lactobacillus plantarum tannase gene and the mutant gene thereof, oriented production and application of tannase with a high yield and high purity can be achieved, and a mutant gene expression product has high catalysis activity upon ester bonds and depside bonds.

The invention discloses Daldinia childiae L2Ma and application thereof in preparation of bacterium grain wood, belonging to the technical field of microorganisms. The strain is collected in China General Microbiological Culture Collection Center; the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing City; and the collection number is CGMCC No:5954. The strain has excellent impregnation capability for a wood-based material, the impregnation time is short, and the impregnation depth is large; beautiful colors and grains can be formed on the surface of the material; and the physical mechanical strength is not influenced. A method for preparing a bacterium grain wood by using the strain comprises the steps of strain activation, expanding propagation and inoculation culture; and the method comprises the following specific steps: inoculating the strain on an activation and expanding propagation culture medium, performing activation and expanding propagation at 22-30 DEG C for 7-15 days, sterilizing the wood-based material, enabling the wood-based material to be sufficiently in contact with the strain subjected to activation and expanding propagation, and culturing at 22-30 DEG C for 6-8 weeks. According to the invention, the method is reasonable in process and simple to operate; and the prepared bacterium grain wood has favorable decoration effect and has no influence on the physical mechanical property of the base material, thereby being beneficial to large-scale production and popularization.

The invention discloses bacillus subtilis wettable powder. Every 1000g of the wettable powder is prepared from the following raw materials: 800g of bacillus subtilis, 450g of distilled water, 250g ofglucose, 150g of peptone, 5g of sodium chloride, 200g of a beef extract and 20g of agar. The bacillus subtilis wettable powder has the beneficial effects that due to addition of the agar and the peptone in production raw materials of the wettable powder, cultured bacillus subtilis can be subjected to solidification protection operation of the agar in the bacillus subtilis culture process, and growth of the peptone can be accelerated after being put into a culture dish; a mixing machine used in a preparation process disclosed by the invention is provided with a lifting clamping ring, a lower bucket base is driven to move upwards or downwards on the inner side of a mixing bucket, an internal depth of the mixing bucket can be adjusted, and then the mixing bucket can be randomly adjusted and operated according to numbers of raw materials to be processed; meanwhile, together with a rotating bottom tube at the bottom of the lower bucket base, raw materials of the mixing bucket can be flexibly conveyed and operated, and the application range of the mixing bucket can be widened.

A distinct Malbec grape variety from Mendoza Argentina, herewith denominated ‘Catena Malbec Clone 16’ which shows a unique vineyard and winemaking profile from the rest of the Malbec grapevine population in Mendoza, Argentina. This Malbec grape variety is characterized by its small cluster size and medium cluster weight; very compact cluster form; very small berry size and weight; medium vigor; medium to high level of millendrage (shot berries); very high level of polyphenols and tannins; very high aromatic intensity and mid palate flavor depth.

The invention discloses a method for screening flesh colors of seed plants of root vegetables. The method includes harvesting the seed plants of the root vegetables and screening the flesh colors before the seed plants live through the winter; sampling the seed plants by the aid of special root vegetable samplers at sampling depths reaching flesh color root center; observing the flesh colors of sampled positions, screening the flesh colors according to target requirements, reserving the sampled positions as seeds for planting and restoring the sampled positions; smearing each sampled position twice with film mulching agents (comprising zhongshengmycin, trichoderma harzianum, guar gum, pullulan and glycerin components), then allowing the seed plants to stand still for approximately 1 day, storing the seed plants in refrigeration houses or cellars to allow the seed plants to live through the winter and colonizing the root plants in the next spring. The method has the advantages that problems such as high storage quantities of seed plants of root vegetables in the prior art, time and labor wastage in flesh color screening procedures and material loss due to the fact that the screened seed plants are easily infected by diseases can be solved; the method for screening the flesh colors of the seed plants of the root vegetables is mature, convenient and speedy, the flesh colors of the original seed plants can be quickly screened in root vegetable seed breeding procedures, and the seeds can be assuredly smoothly harvested.

The invention relates to a catalyst for converting sulfur-containing polycyclic aromatic hydrocarbons into monocyclic aromatic hydrocarbons and a preparation method thereof. The method mainly solves the problems of low conversion depth of sulfur-containing polycyclic aromatic hydrocarbons, low yield and low selectivity of single-ring aromatic hydrocarbons, and fast catalyst deactivation rate existing in the prior art. The present invention comprises the mixture of 30~65% FAU type zeolite and ZSM-12 molecular sieve by adopting catalyst by weight percentage, 33.5~69.8% is selected from at least one in γ-alumina, η-alumina or pseudo-boehmite One is the technical scheme of binder and 0.1-0.5% of at least one metal selected from Pt, Pd or Ir and 0.1-1% of at least one metal selected from La, Ce or Sn, which better solves the problem To solve the above problems, the catalyst can be used in the industrial production of converting sulfur-containing polycyclic aromatic hydrocarbons into single-ring aromatic hydrocarbons.

The invention discloses a method for extracting and measuring content of apigenin and xanthophyll in chrysanthemum morifolium ramat. The method is characterized by comprising the following steps: drying the chrysanthemum morifolium ramat, grinding, adding the crushed chrysanthemum morifolium ramat into an organic solvent for extraction according to a material-liquid ratio of (1:150)-(1:20), repeatedly extracting for 1-5 times, filtering extracting solution, and performing vacuum concentration until the solution evaporate to be dry, so as to obtain the apigenin and the xanthophyll. The measurement method adopts a high performance liquid chromatography. The operation is simple, the content of two substances, such as the apigenin and the xanthophylls, in the chrysanthemum morifolium ramat can be simultaneously extracted and measured, the complexity of respective extraction and measurement is reduced, the loss of active substances is reduced to the greatest degree, and a theoretical basis is provided for further popularization and deep processing of related chrysanthemum morifolium ramat products.

The invention provides a method for hydrolyzing propolis flavonoid glycoside by naringinase. The method is as follows: naringinase is adopted to carry out constant-temperature enzymolysis of propolis flavonoid glycoside; propolis flavonoid glycoside substance is hydrolyzed into flavonoid aglycone through enzymolysis; moreover, compared with raw materials, the antioxidation property of the productis reinforced. The content of flavonoid substances in the obtained enzymolysis products can be measured through high-efficiency liquid phase chromatogram, and the result shows that the content of flavonoid glycoside substances is reduced, while the content of flavonoid aglycone substances is obviously increased. The method realizes deep development and utilization of propolis products, and is propitious to increase the added value of bee products and expand the application range of bee products. Moreover, the made propolis enzymolysis products has remarkably increased antioxidation property, and can be better used in the fields of medicine, foodstuff and cosmetics, etc. as compared with raw material propolis.

The invention discloses a method for separating and purifying macromolecular phenolic acids in tree moss extract. According to the physical and chemical properties of the functional groups in the molecular structure of tree moss extract, the aprotic polarity of specific molecular structure is selected. Non-toxic organic solvent, dissolves and extracts all the easily soluble small molecular aroma substances in the tree moss extract, and at the same time makes the macromolecular substances of depsipolic acids precipitate in the solvent due to poor solubility, reaching the small molecular substances and The effect of complete separation of macromolecular phenolic acids. The isolated depsipolic acid solids are purified by recrystallization from a suitable organic solvent to obtain depsipolic acid solids with lighter color and higher purity. The separation method provided by the invention can improve the use value of the tree moss extract and expand its application field. The purified depsipated phenolic acid solid is in the form of granules, which is loose, dry, non-sticky, easy to use and add, etc. It has a good application prospect in both traditional cigarettes and new tobaccos.

The invention discloses a method for generating aglycon by converting grifola frondosa into soybeam isoflavone glycoside by fermenting. The method comprises the following steps: inoculating grifola frondosa to a culture medium to carry out fermentative culture, so that grifola frondosa mycelium fermentation liquor is obtained; carrying out homogenate treatment on the fermentation liquor, so that a grifola frondosa mycelium is broken, and beta-glucosidase is released to the fermentation liquor, thereby obtaining crude enzyme; and mixing the crude enzyme with soybeam isoflavone glycoside, and reacting the obtained mixture, so that soybean isoflavone glycoside is obtained. According to the method disclosed by the invention, through the deep fermentation of grifola frondosa, a large amount of beta-glucosidase is obtained, and then aglycon is prepared by converting the beta-glucosidase into soybeam isoflavone glycoside, the activity of the obtained beta-glucosidase is high, the product conversion rate is high, and the activity and absorption rate of soy isoflavone are greatly improved; and the converted product is also rich in biological active substances such as grifola frondosa ectoenzyme and mycelia polysaccharides, thereby laying a foundation for the development and industrial production of novel function materials.

The invention discloses a method for controlling rice-stem borers. The method comprises the following steps: 1, placing a trapping basin in every 20m<2> of a rice field, and adding water to each trapping basin to reach a position 1 to 2cm away from the upper edge of the trapping basin, wherein each trapping basin is a circular open trapping basin which has an open area of 0.2 to 0.4m<2> and a depth of 8 to 10cm; 2, spraying about 0.1 to 0.5g of sex pheromone compound of rice-stem borers to the water surface of each trapping basin to which water is added, wherein the sex pheromone compound of the rice-stem borers is prepared from the following components in parts by weight: 40 parts of cis-11-hexadecanal, 8 parts of cis-13-octadecanal, 5 parts of cis-9-hexadecanal, anthocyanin, glycerol and ethyl acetate. According to the method disclosed by the invention, male rice-stem borers are trapped by using the sex pheromone compound of the rice-stem borers, such that the number of a second generation of the rice-stem borers can be remarkably reduced; the sex pheromone compound of the rice-stem borers is directly sprayed during the use, can float on the surface of the water, is suitable for release of sex pheromone of the rice-stem borers, and has a good trapping effect and no pollution to the environment.

Methods for modulating the level of a chemokine in a cell by administering to a cell an effective amount of a depside or an anthocyanin are provided. More particularly, a method for modulating the level of a chemokine in a cell by administering to a cell an effective amount of a depside having the structure of formula (IV): Formula (IV) wherein R is selected from H or CH3 or an anthocyanin selected from cyanidin 3-glucoside, delphinidin 3-glucoside, or combinations thereof, or an enantiomer, optical isomer, diastereomer, N-oxide, crystalline form, hydrate, or pharmaceutically acceptable salt thereof is provided. Also provided are compounds according to Formulas I-IV, pharmaceutical compositions, unit dosage forms, and food or feed supplements containing such compounds. Methods for treating a condition in a mammal and for treating or ameliorating a condition, such as for example, chronic obstructive pulmonary disease (COPD) by administering an effective amount of a composition containing such compounds are also provided. Further provided is an extract obtained from the fruit of Myrciaria cauliflora containing at least one compound of the present invention in substantially pure form.

The present invention relates to medicine engineering technology, and is especially anticancer active compounds depside peptides as the derivatives of natural production. The compounds of the present invention, Cryptophycins I-V, are prepared through one synthesis path including separate synthesis of four molecular fragments, connecting these fragments to form 16-element macrolide compound, and final double bond epoxidation. Compared with Cryptophycin 52, Cryptophycins I-V have specific group introduced to some specific position and thus raised water solubility and stability.

The invention discloses a WPGS 1 gene and a WPGS 2 gene derived from eucalyptus and encoding protein and application thereof and belongs to the field of biotechnology. Ribonucleic acid (RNA) in the eucalyptus is extracted and is subjected to reverse transcription to be complementary deoxyribonucleic acid (cDNA), deep sequencing is performed to obtain an overall length cDNA sequence of the eucalyptus, the similarity between a deoxyribonucleic acid (DNA) sequence and the eucalyptus cDNA sequence library sequence obtained through sequencing is compared to obtain overall length cDNA sequences of the WPGS 1 gene and the WPGS 2 gene in the eucalyptus and lead the sequences into Arabidopsis, wherein the DNA sequence is presumed according to AtGA-20 and AtEXO protein amino acid sequences of arabidopsis published by center of biotechnology information (NCBI). The biomass of transgenic plants is remarkably improved through over-expression of the WPGS 1 gene and the WPGS 2 gene.

The invention discloses a separation and purification method of macromolecular depside in a tree moss extract. The method comprises the following steps: selecting an aprotic polar organic solvent with a specific molecular structure according to the physicochemical properties of functional groups in the molecular structure of a depside compound of the tree moss extract, and completely dissolving and extracting micromolecular aroma substances which are easy to dissolve in the tree moss extract. Meanwhile, the depside macromolecular substances are separated out in the solvent due to poor dissolving capacity, so that the effect of completely separating the small molecular substances from the macromolecular depside substances in the tree moss extract is achieved. The depsidesolid obtained by separation is recrystallized and purified through a proper organic solvent, and the depside solid with light color and high purity can be obtained. The separation method provided by the invention can improve the use value of the tree moss extract and expand the application field of the tree moss extract. The purified depside solid is granular, has the characteristics of being loose, dry, non-viscous, convenient to use and add and the like, and has a better application prospect in traditional cigarettes and novel tobaccos.

The invention discloses a method for catalyzing asymmetric hydrolysis of 1,2-epoxyoctane by soybean epoxide hydrolase and belongs to the field of biochemical industry. With soybean epoxide hydrolase as a catalyst and a deep cosolvent as a solvent, asymmetric hydrolysis of 1,2-epoxyoctane is enzymatically catalyzed to prepare chiral octanediol. The method has the advantages of mild conditions, high synthetic yield, operation simplicity and the like and has good industrial value.

The invention discloses a depsidone compound. A structural formula of the depsidone compound is shown in a formula I in the description. The depsidone compound is occasionally separated out when an inventor separates a fermenting product of aspergillus unguis 6-20-6. Proofed by experiment, the depsidone compound has the effect of inhibiting pseudomonas aeruginosa, methicillin-resistant staphylococcus aureus and candida albicans, and can be used for preparing anti-bacterial agents or anti-fungi agents.