31 results about "Stereum hirsutum" patented technology

The invention relates to a method for preparing gingko golden fungus fruit wine, which comprises the following steps that: golden fungus type spore and monoxenous stereum hirsutum parent strains are used for carrying out liquid bottle shaking seed culture of the golden fungus type spores, the seed tank culture of the golden fungus type spores, the bottle shaking seed culture of the autoecious stereum hirsutum, the bottle shaking expansion culture of the autoecious stereum hirsutum and the seed tank culture of the autoecious stereum hirsutum, the seed tank seed of the golden fungus type sporesand the seed tank seed of the autoecious stereum hirsutum are subjected to the mixed fermentation culture, ginkgo starch substrates and wine brewing yeasts are added into the mixed fermentation liquid of golden fungus type spores and the autoecious stereum hirsutum for carrying out post fermentation, the gingko golden fungus fruit wine with golden fungus extracellular polysaccharide, stereum hirsutum terpene, ginkgo flavone and bilobalide b can be obtained, the alcohol volume is higher than 3 percent, the ginkgo phenolic acid is smaller than 1ppm, and the hydrocyanic acid is lower than 0.1microgramme per liter. The gingko golden fungus fruit wine obtained by the method has an anti-radiation health care function.

The invention discloses a preparation method of a cultivated strain of tremella aurantialba. The preparation method is characterized by comprising steps as follows: strain culture: solid culture and liquid culture of Stereum hirsutum as a first component of the cultivated strain of the tremella aurantialba; test tube seed culture, liquid seed culture, shaking culture and fermentation tank culture of tremella aurantialba yeast-like conidia as a second component of the cultivated strain of the tremella aurantialba; mixing of the first component and the second component of the cultivated strain of the tremella aurantialba; processes of cut-log cultivation and cultivation in a bag or a bottle. The tremella aurantialba and the companion fungi, namely the Stereum hirsutum, in the cultivation of the tremella aurantialba are cultured respectively, the tremella aurantialba and the companion fungi can be sufficiently and uniformly mixed, so that the yeast-like conidia of the tremella aurantialba can germinate sufficiently, the frequent condition that a large quantity of Stereum hirsutum sporocarps grow on cut-logs in the cultivation of the tremella aurantialba can be prevented, with the application of the strain, the fruiting rate of the cut-log cultivation of the tremella aurantialba is higher than 95%, and the yield is increased by 15%-20%; with the application of the liquid-liquid strain of the tremella aurantialba, the tremella aurantialba and the companion fungi are uniformly mixed, during cultivation in the bag or the bottle, the tremella aurantialba spawn running is fast, the colonization is fast quickly, the pollution rate is low, the fruiting rate is up to 100%, the color change is fast, and the growing period is short.

The invention belongs to the field of edible fungi cultivation, and particularly relates to a direct regeneration culture method for tremella aurantia fruiting bodies. Tissue blocks of the tremella aurantia fruiting bodies are cultured individually, or are cultured together with exogenous stereum hirsutum, and thereby, the tissue blocks of the small tremella aurantia fruiting bodies directly growinto large tremella aurantia fruiting bodies. The culture method can improve the success rate of making of a tremella aurantia strain and the inoculation success rate of tremella aurantia cultivationbags, shortens the culture period, and is beneficial to the modernized and industrialized cultivation of tremella aurantia.

The invention relates to a method for preparing a gingko and tremella mesenterica fruit wine through fermentation by taking gingko as a substrate. The method comprises the following steps of: respectively culturing tremella mesenterica yeast spores and a host bacterium of Stereum hirsutum to obtain seed liquors; performing mixed fermentation culture on the seed liquor of the tremella mesenterica yeast spores and the seed liquor of the host bacterium of Stereum hirsutum to obtain a fermentation liquor; and mixing the fermentation liquor with a ginkgo starch substrate and Saccharomyces cerevisiae, and fermenting to obtain the gingko and tremella mesenterica fruit wine. The alcoholic strength of the prepared gingko and tremella mesenterica fruit wine is over 3 percent, the ginkgo acid is less than 1ppm, and the hydrocyanic acid is less than 0.1 milligram / liter. The gingko and tremella mesenterica fruit wine prepared by the method has radioresistant health-care effect.

The invention relates to a DNA sequence of a fungal strain and a method for identifying variety by means of the sequence. An ITS zone sequence of tremella aurantiabla is shown in SEQ ID NO.1, and an ITS zone sequence of host fungus stereum hirsutum is shown in SEQ ID NO.2. During identification, an ITS zone sequence of a to-be-identified sample is extracted at first and then is respectively compared with the SEQ ID NO.1 sequence and the SEQ ID NO.2 sequence to obtain a result. The method can accurately identify the authenticity of a to-be-identified fungus, and can also carry out accurate identification to artificially cultured yeast-like spore strain of tremella aurantiabla and host fungus stereum hirsutum strain; moreover, the method can accurately distinguish and identify the authenticity and purity of tremella aurantiabla variety or strain, thereby providing a reliable technique for variety identification, protection, strain quality control and effective strain breeding.

The invention relates to an inoculated culture method for a liquified strain of tremella auramtialba. A beaten tremella auramtialba fruit body is mixed with a stereum hirsutum liquid strain to preparea liquified strain, or the tremella auramtialba fruit body is put into the stereum hirsutum liquid strain to be directly beaten to prepare the liquified strain. A tremella auramtialba culture is inoculated by using the liquid strain, so that tremella auramtialba regeneration points in the inoculated tremella auramtialba culture can be greatly increased, the inoculation success rate of the tremella auramtialba culture is improved to over 99% and the culture cycle is shortened to 60-65 days.

The invention discloses a method for synthesizing ginkgolide B through converting a ginkgolide mixture by using stereum hirsutum thalli as a catalyst, belonging to the field of biotechnologies. The method comprises the following steps: carrying out amplification culture on stereum hirsutum strains, taking thalli which are obtained through a test tube slant, a liquid shake flask, seed culture and thallus separation as the catalyst, converting ginkgolide into ginkgolide B, and enabling a conversion solution to be subjected to centrifugation, ethyl acetate extraction and anhydrous alcohol crystallization, so as to separate ginkgolide B from fermentation liquor. By using the method, the purity of obtained ginkgolide B is not lower than 99%, and the ginkgolide conversion ratio is higher than 90%.

The invention discloses a high-yield strain production cultivation method of golden fungus. The high-yield strain production cultivation method comprises the following steps: preparing a mother strain culture medium, sterilizing the mother strain culture medium, preparing mother strains, preparing a stock (cultivation strain) culture medium, preparing stock (cultivation strain) inoculation and cultivating with materials for substitutes. A formula of the mother strain culture medium of the golden fungus is prepared from (peeled) potato, sucrose, potassium dihydrogen phosphate, magnesium sulfate, agar and water at the ratio of 20 to 3 to 0.3 to 0.15 to 100. A formula of a stock (cultivation strain) culture medium is prepared from 80 percent of broad-leaved tree sawdust, 16 percent of corn flour, 2 percent of sucrose, 1 percent of potassium dihydrogen phosphate and 1 percent of gypsum. By carrying out two times of strain preparation (mother strain preparation and stock preparation), the golden fungus and accompanying stereum hirsutum are separated and are cultured respectively, so as to obtain a golden fungus mixed stock. In a cultivating process, the germination of strains is rapid and material consumption is rapid; the pollution rate is low and a color changing speed is rapid; the growth period is short.

The invention discloses a binary cultivation method of bagged tremella aurantialba, and belongs to the technical field of edible fungus cultivation. The method includes the steps of cultivation of mixed mycelia of tremella aurantialba and stereum hirsutum, manufacturing of cultivation bags, first inoculation, first-stage cultivation of the mycelia, second inoculation, sealing, second-stage cultivation of the mycelia and fruiting management. The method solves the problems that in current development of the industry of tremella aurantialba, when primordia of tremella aurantialba are formed, severe damage caused by corrosion, withering, diseases and pest disasters is likely to occur, the fruiting rate of tremella aurantialba is increased, the operation is simple, the circle is short, the costis low, the standards are controllable, and large-scale standard commercial production of tremella aurantialba can be achieved.

The invention belongs to the field of edible fungus cultivation, and particularly relates to a tremella aurantialba cultivation method of performing inoculation with tremella aurantialba seedlings. Small containers are utilized to make the tremella aurantialba seedlings, and the tremella aurantialba seedlings which are covered with hyphae and have established a parasitic relation and on which small tremella aurantialba grow out serve as inoculation materials for inoculating cultivation bags; the tremella aurantialba seedlings with or without containers are inoculate to the cultivation bags; when the cultivation bags are inoculated with the tremella aurantialba seedlings, a liquid strain of host stereum hirsutum of the tremella aurantialba is used or not used as a spawn runing aid; after the cultivation bags are inoculated with the tremella aurantialba seedlings, mycelium parts of the tremella aurantialba seedlings grow directly, and culture materials in the cultivation bags are decomposed to supply nutrients to the small tremella aurantialbas above the culture materials to make the small tremella aurantialbas continue to grow, and thus, tremella aurantialba fruit body products canbe obtained. By applying the method, the inoculation success rate of the tremella aurantialba cultivation bags is increased to 99% or more, and the cultivation time of the tremella aurantialba cultivation bags is shortened to 45-55 days.

The invention belongs to the field of edible mushroom cultivation, and relates to a culture method of golden fungus fruiting bodies through fusion and concrescence. The culture method is characterizedin that in a golden fungus culture process, golden fungus fruiting body tissue blocks are used as inoculation materials, exogenous stereum hirsutum is provided for the inoculation materials, and fusion occurs between mycelia of stereum hirsutum in the golden fungus tissue blocks and mycelia of the exogenous stereum hirsutum, so that the exogenous stereum hirsutum is used as a nutrition supply source of the golden fungus fruiting bodies, and the golden fungus fruiting body tissue blocks re-obtain nutrition supply and recover growth and development to form new complete golden fungus fruiting bodies. According to the culture method disclosed by the invention, stereum hirsutum strains and golden fungus fruiting body strains are separately cultured, when golden fungus mother strains, originalstrains, cultivated strains and / or cultivated bags are made, inoculation is performed by a synchronous method or a split-step method randomly, and the inoculation success rate of strains and cultivated bags at all levels is high.

The invention discloses application of stereum hirsutum thalli in preparation of ginkgolide B. According to the application, a method for preparing ginkgolide B comprises the following steps: carrying out amplification culture on stereum hirsutum strains, taking thalli which are obtained through a test tube slant, a liquid shake flask, seed culture and thallus separation as a catalyst, converting ginkgolide into ginkgolide B, and enabling a conversion solution to be subjected to centrifugation, ethyl acetate extraction and anhydrous alcohol crystallization, so as to separate ginkgolide B from fermentation liquor. By using the method, the purity of obtained ginkgolide B is not lower than 99%, and the ginkgolide conversion ratio is higher than 90%.

The invention relates to a molecular identification method of a ribosome large-subunit gene D1 / D2 region sequence of a golden tremella and a host fungi crude Stereum hirsutum. The ribosome large-subunit gene sequence of the golden tremella is shown in SEQ ID NO.1, and the ribosome large-subunit gene D1 / D2 region sequence of the host fungi crude Stereum hirsutum is shown in SEQ ID NO.2. The molecular identification method has the steps as follows: the ribosome large-subunit gene D1 / D2 region sequence of the strains to be identified is obtained after extraction, and is judged through being compared with the sequence in the SEQ ID NO.1 and the SEQ ID NO.2, so as to judge whether the strains to be identified is the golden tremella strains or host fungi crude Stereum hirsutum strains. By utilizing the molecular identification method, the golden tremella strains and the host fungi crude Stereum hirsutum strains which are cultured artificially can be identified accurately; the reliable technology is provided for strains identification, breed conservation, strain quality control, effective strain propagation, and artificial cultivation of the golden tremella.

The invention discloses a method for synthesizing bilobalide B by using Stereum hirsutum thalli as a catalyst to convert a bilobalide mixture, belonging to the biotechnical field. The method disclosed by the invention comprises the following steps: carrying out enlargement cultivation on the Stereum hirsutum thalli; converting bilobalide to synthesize the bilobalide B by using the thalli as a catalyst by means of a test tube slant, a liquid shake flask, seed culture and thallus separation; and centrifugalizing the conversion liquid, extracting by ethyl acetate and crystallizing by anhydrous alcohol to separate the bilobalide B from fermentation liquor. The purity of the bilobalide B obtained by the method is greater than or equal to 99%, and the bilobalide conversion ratio is greater than 90%.

The invention relates to a method for breeding a new tremella aurantialba variety. The method comprises the steps that asexual spores and stereum hirsutum in tremella aurantialba strains from different producing areas are separated, cross combination and collocation are carried out on the asexual spores and stereum hirsutum of the tremella aurantialba from different sources, so that excellent properties of female parents of two species are combined into a combined tremella aurantialba sporocarp strain, then, character stability of the tremella aurantialba is maintained through asexual propagation so as to be applied to production, and therefore new strains and new varieties of the tremella aurantialba are formed. The method provides a feasible way for breeding of tremella aurantialba strains or varieties, and tremella aurantialba recombinant strains with high yield and disease resistance are obtained by using the method to breed the tremella aurantialba strains.

The invention discloses stereum hirsutum fermentation liquor and sesquiterpene which are produced by fermenting stereum hirsutum liquor, as well as applications thereof, and particularly relates to a production technology process of sesquiterpene cultured and obtained by the stereum hirsutum liquor, and the hypoglycemic efficiency of sesquiterpene. Repeated tests and study show that a culture solution produced by the production technology contains sesquiterpene, a mouse with hyperglycemia takes the culture solution orally, and then blood sugar and fructosamine of the mouse can be reduced remarkably, therefore, the stereum hirsutum culture solution is confirmed to have the application for preparing healthy drinks and medicines capable of reducing the blood sugar. The culture solution is liquor with the color ranging from aurantium to tawny, and the sesquiterpene content in the culture solution is 0.4 mg / mL-4.0 mg / mL. According to the invention, not only is a new way explored for the deep development and utilization of the stereum hirsutum, but also an ideal traditional Chinese medicine is provided for controlling the blood sugar.

The invention discloses an efficient tremella aurantialba liquid strain inoculation cultivation method. The method comprises the following steps of inoculating a stereum hirsutum strain into a culturemedium, and then putting the culture medium into a 23-27 DEG C shaking table for culturing for 4-6 days to obtain a tremella aurantialba liquid strain; and inoculating the tremella aurantialba liquidstrain into a cultivation bag or a cultivation bottle for dark culture, grafting a tremella aurantialba tissue block to a cultivation material inoculation hole or the material surface of a half-bag fungus bag after hyphae germinate, continuously performing dark culture until fruiting, performing fruiting management in an illumination environment until maturity, and picking. According to the method, tremella aurantialba liquid strain cultivation is adopted, the period can be shortened, the tremella aurantialba yield can be effectively guaranteed, the short period, the high tremella aurantialbayield, the low pollution rate, the low sporocarp difference rate, the high quality and the like of tremella aurantialba artificial cultivation are achieved, and therefore high-quality artificial cultivation of tremella aurantialba is achieved. The invention provides a liquid culture medium formula, a cultivation material formula, a liquid strain preparation method and six cultivation management methods.

The invention discloses a preparation method and an application of stereum hirsutum mycelium polysaccharide, relates to the field of biological fermentation and medicines, and particularly relates to a production technology process for extracting mycelium polysaccharide by obtaining mycelium through liquid culture of stereum hirsutum, and blood sugar and blood fat reduction effects of the polysaccharide. Repeated experimental study proves that the mycelium polysaccharide produced by applying the production process can obviously reduce the levels of blood sugar and blood fat in hyperglycemic mice through intraperitoneal injection or oral administration, thus proving that the stereum hirsutum mycelium polysaccharide can be applied to the preparation of medicines for reducing blood sugar and blood fat. The preparation method opens the bran-new approach for further development and utilization of stereum hirsutum and can be used for providing a novel ideal traditional Chinese medicine for the reduction of blood sugar and blood fat.

The invention relates to the technical field of biology, and particularly provides a growth promotion preparation capable of increasing content of ginkgolide B in gingko leaves. The growth promotion preparation is composed of, by weight, 1.5-3.5 parts of Stereum hirsutum, 5.6-7.8 parts of Fusarfum tricinctum, and 15.0-20.0 parts of distilled water. The invention also relates to a preparation method and an application of the growth promotion preparation. According to the growth promotion preparation, instead of single separation and purification methods for acquiring a large amount of the ginkgolide B, by adding ginkgolide to a culture medium, bacteria are prepared through liquid culture technology with the Stereum hirsutum and Fusarfum tricinctum as bacterial strains, wherein the bacteria,as a catalyst, can convert ginkgolide A, ginkgolide C and ginkgolide M into the ginkgolide B. The growth promotion preparation greatly increases economic value and medicinal value of the product.

The invention discloses a method for preparing rare ginsenoside through solid-state fermentation of stereum hirsutum and cellulase, and belongs to the technical field of biological preparation of theginsenoside. According to the method, the stereum hirsutum is taken as a fermentation strain, a seed solution is inoculated into a culture medium which takes agricultural waste as a fermentation substrate and contains ginseng extract and cellulase, solid-state fermentation culture is performed for 10 days, and a product rich in ginsenoside Rd, F2, CK, PPT and other rare saponins is obtained afterfermentation is finished, and meanwhile, the fermentation product also contains a plurality of bioactive substances such as stereum hirsutum polysaccharides, sterols, esters and sesquiterpenes. The method is simple in process, low in fermentation cost and high in conversion efficiency, provides a reference for circular biological economic production of the bioactive substances containing various rare ginsenosides, and provides a method for developing a new technology for preparing various high-value health products by utilizing fermentation of the stereum hirsutum.

The invention belongs to the field of industrial cultivation of edible fungi, and particularly relates to an industrial three-stage cultivation method for tremella aurantialba. According to the biological characteristics of the tremella aurantialba, the whole culture process of the tremella aurantialba is accurately divided into three stages, namely the stereum hirsutum growth and tremella aurantialba field planting stage which is 180 hours after inoculation, the fruiting body rapid growth stage which 180 hours to 780 hours after inoculation; and the fruiting body coloring stage which is 780 hours to 1152 hours after inoculation. Through continuous 20 times of verification tests, results show that the cultivation method greatly improves the survival rate and yield of fruiting bodies of thetremella aurantialba, and the average biological efficiency reaches 70% or above.

The method comprises the following steps: taking an upper-layer material in a tremella aurantialba sporocarp stock seed bottle or an upper-layer material in a cultivation seed bag, inoculating the upper-layer material into a special culture medium, and generating an outer ring and an inner ring of the stereum hirsutum with obvious antagonism lines in a culture dish; picking the Stereochaete hirsutum, and then inoculating the Stereochaete hirsutum into a special culture medium for purification culture until a dish is full; the special culture medium comprises the following components in per liter of water: 3% of aqueous extract of tremella aurantialba sporocarp, 0.2% of yeast extract, 0.6% of malt extract, 8% of rice meal and 20% of agarose; the method comprises the following steps: fermenting an inner-ring stereum hirsutum liquid until mycelium pellets are dense, using the mycelium pellets as a tremella aurantialba fruiting body cultivar, inoculating the tremella aurantialba fruiting body cultivar into a tremella aurantialba cultivation bag, and cultivating to obtain the tremella aurantialba fruiting body. The tremella aurantialba sporocarp cultivated species are high in growth speed, high in size consistency, full and round, ruddy and fleshy, uniform in lines and not hollow, and after the cultivated species are inoculated, tremella aurantialba grows well and is robust.

The invention discloses a method for simultaneously preparing ginkgolide B and quercetin from stereum hirsutum and relates to the technical field of biological engineering. The preparation method includes the steps that fermentation liquor containing ginkgolide B and a mycelium containing quercetin are prepared sequentially through the steps of tube enlarged culture, liquid shake culture, seed tank enlarged culture, solid primary fermentation culture, liquid post-fermentation culture and extraction and separation; ginkgolide B and quercetin crystal are prepared through extraction. Peeled ginkgoes and rice serve as a solid matrix, gingko leaf extracts are added, stereum hirsutum serves as a strain to convert ginkgolide and ginkgetin through solid primary fermentation, then quercetin and ginkgolide B are separated through post-fermentation, large-scale industrial production can be achieved, and ginkgolide B and quercetin in gingkoes can be produced at the same time. The process is simple, the purity of ginkgolide B is 90% or above and the purity of quercetin is 95% or above in the obtained product, and preparation purity is high.