Culture method of golden fungus fruiting bodies through fusion and concrescence

A technology of golden ear fruiting body and cultivation method, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of inappropriate facility, factory fruiting, long cultivation period, irregular fruiting, etc.

Pending Publication Date: 2020-07-03
云南菌视界生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanism of this process has not been studied clearly at present, and it cannot be artificially regulated. Therefore, the ratio of host and parasite is often inappropriate. When making strains, invalid strains and strain states are inconsistent. When inoculating cultivation bags Lead to the occurrence of bacteria packs without mushrooms (25%) and irregular fruiting, and the culture period is as long as 70 to 80 days
[0004] In addition, the existing Auricularia fruiting body culture technology is not suitable for the needs of facilities and industrialized fruiting

Method used

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  • Culture method of golden fungus fruiting bodies through fusion and concrescence
  • Culture method of golden fungus fruiting bodies through fusion and concrescence
  • Culture method of golden fungus fruiting bodies through fusion and concrescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1 strain production

[0047] 1.1 Production of mother species

[0048] 1.1.1 Preparation of mother seed medium

[0049] 1.1.1.1 Mother solid medium formula (1L):

[0050] Potato 200g, sucrose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0051] 1.1.1.2 Formula of stock liquid medium (1L):

[0052] Potato 200g, sucrose 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0053] 1.1.1.3 Preparation method of mother seed medium:

[0054] Calculate the dosage of each component according to the preparation amount, weigh the peeled and sliced ​​potatoes, add water and boil for 15 minutes, then filter with 4 layers of gauze to obtain the filtrate, accurately weigh each medicine, put it into a container that can be fixed, add the filtrate and adjust the volume to the prepared amount , Stir to fully dissolve and evenly disperse various medicines. For the production of petri dishes, divide them into Erlenmeyer flasks and seal them with a gas-permeable film, and then sterilize them ...

Embodiment 2

[0120] 1 strain production

[0121] 1.1 Production of mother species

[0122] 1.1.1 Preparation of mother seed medium

[0123] 1.1.1.1 Mother solid medium formula (1L):

[0124] Soybean powder 5g, sucrose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0125] 1.1.1.2 Formula of stock liquid medium (1L):

[0126] Soybean powder 5g, sucrose 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0127] 1.1.1.3 Preparation method of mother seed medium:

[0128] Calculate the dosage of each component according to the preparation amount, accurately weigh each drug, put it into a container that can be constant, add water and set the volume to the preparation amount, stir to fully dissolve and evenly disperse each drug. Put the petri dishes into Erlenmeyer flasks and seal them with a gas-permeable film, then sterilize them with damp heat and high pressure (121°C) for 25 minutes, and then put them on the ultra-clean bench while they are still hot and put them into sterile petri dishes, and th...

Embodiment 3

[0194] 1 strain production

[0195] 1.1 Production of mother species

[0196] 1.1.1 Preparation of mother seed medium

[0197] 1.1.1.1 Mother solid medium formula (1L):

[0198] Yeast powder 5g, glucose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0199] 1.1.1.2 Formula of stock liquid medium (1L):

[0200] Yeast powder 5g, glucose 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0201] 1.1.1.3 Preparation method of mother seed medium:

[0202] Calculate the dosage of each component according to the preparation amount, accurately weigh each drug, put it into a container that can be constant, add water and set the volume to the preparation amount, stir to fully dissolve and evenly disperse the various drugs. Put the petri dishes into Erlenmeyer flasks and seal them with a gas-permeable film, then sterilize them with damp heat and high pressure (121°C) for 25 minutes, and then put them on the ultra-clean bench while they are still hot and put them into sterile petri dishes, an...

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Abstract

The invention belongs to the field of edible mushroom cultivation, and relates to a culture method of golden fungus fruiting bodies through fusion and concrescence. The culture method is characterizedin that in a golden fungus culture process, golden fungus fruiting body tissue blocks are used as inoculation materials, exogenous stereum hirsutum is provided for the inoculation materials, and fusion occurs between mycelia of stereum hirsutum in the golden fungus tissue blocks and mycelia of the exogenous stereum hirsutum, so that the exogenous stereum hirsutum is used as a nutrition supply source of the golden fungus fruiting bodies, and the golden fungus fruiting body tissue blocks re-obtain nutrition supply and recover growth and development to form new complete golden fungus fruiting bodies. According to the culture method disclosed by the invention, stereum hirsutum strains and golden fungus fruiting body strains are separately cultured, when golden fungus mother strains, originalstrains, cultivated strains and / or cultivated bags are made, inoculation is performed by a synchronous method or a split-step method randomly, and the inoculation success rate of strains and cultivated bags at all levels is high.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a method for fusion and synbiotic cultivation of auricularia fruiting bodies. Background technique [0002] In terms of biological characteristics, golden ear is a parasitic fungus, parasitic on Bacillus chamomile, and the fruiting body of golden ear is a heterogeneous fruiting body, which is a complex composed of Auricularia hyphae and Mycelium chamomile (That is, the golden ear fruit body itself contains two species simultaneously), and the growth of the golden ear hyphae will rely on the hairy bacteria to provide nutrition for it. Based on the guidance of this principle, the existing golden ear cultivation process [1,2,3] Such as figure 1 shown. [0003] In short, in the cultivation technique of existing golden ear (see figure 1 ), inoculate with a mixed culture of the host and parasite, or inoculate with a single culture of the two. After inoculating i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/40
CPCA01G18/00A01G18/20A01G18/40
Inventor 李荣春杨林雷
Owner 云南菌视界生物科技有限公司
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