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Direct regeneration culture method for tremella aurantia fruiting bodies

A golden ear fruiting body, regeneration culture technology, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of inappropriate proportioning, fruiting of no fruiting mushrooms, inconsistency between ineffective strains and strains, etc. Achieve the effect of shortening the culture period and improving the success rate of inoculation

Inactive Publication Date: 2020-06-26
云南菌视界生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current culture process, pure Auricularia hyphae and Mycelium chamois are transferred to the medium in the form of a mixture, and the parasitic relationship needs to be re-established. The mechanism of this process has not been studied clearly, so it often occurs The inappropriate situation of the two ratios will cause inconsistencies in the status of invalid strains and strains when making strains, and will cause bacterial bags (25%) without fruiting and irregular fruiting (fruiting bodies) to occur when inoculating cultivation bags. The consistency rate of the maturity of a tidal mushroom is 40%), and the culture period is as long as 70-80 days
In addition, the existing Auricularia fruiting body culture technology is not suitable for the needs of modernization and factory fruiting

Method used

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  • Direct regeneration culture method for tremella aurantia fruiting bodies
  • Direct regeneration culture method for tremella aurantia fruiting bodies
  • Direct regeneration culture method for tremella aurantia fruiting bodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1 strain production

[0032] 1.1 Golden ear fruiting body parent species

[0033] 1.1.1 Auricularia fruiting body stock culture medium formula (1L):

[0034] Potato 200g, sucrose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0035] 1.1.2 Method for preparing culture medium for mother species of Auricularia fruiting body:

[0036] Peeled potato slices, add water and boil for 15 minutes, then filter with 4 layers of gauze to obtain the filtrate, accurately weigh each drug, put it into a container that can be constant, add the filtrate and set the volume to the prepared amount, stir to fully dissolve and evenly disperse the various drugs . Put the petri dishes into Erlenmeyer flasks and seal them with a gas-permeable film, then sterilize them with damp heat and high pressure (121°C) for 25 minutes, and then put them on the ultra-clean bench while they are still hot and put them into sterile petri dishes, and then use them after cooling; For the inclined plane o...

Embodiment 2

[0062] 1 strain production

[0063] 1.1 Golden ear fruiting body parent species

[0064] 1.1.1 Auricularia fruiting body stock culture medium formula (1L):

[0065] Yeast powder 5g, glucose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0066] 1.1.2 Method for preparing culture medium for mother species of Auricularia fruiting body:

[0067] Calculate the dosage of each component according to the preparation amount, accurately weigh each drug, put it into a container that can be constant, add water and set the volume to the preparation amount, stir to fully dissolve and evenly disperse the various drugs. Put the petri dishes into Erlenmeyer flasks and seal them with a gas-permeable film, then sterilize them with damp heat and high pressure (121°C) for 25 minutes, and then put them on the ultra-clean bench while they are still hot and put them into sterile petri dishes, and then use them after cooling; For the inclined plane of the test tube, heat it to dissolve the a...

Embodiment 3

[0094] 1 strain production

[0095] 1.1 Golden ear fruiting body parent species

[0096] 1.1.1 Auricularia fruiting body stock culture medium formula (1L):

[0097] Soybean powder 5g, sucrose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0098] 1.1.2 Method for preparing culture medium for mother species of Auricularia fruiting body:

[0099] Accurately weigh each drug and put it into a volume-constant container, add water and adjust the volume to the prepared amount, stir to fully dissolve and evenly disperse various drugs. Put the petri dishes into Erlenmeyer flasks and seal them with a gas-permeable film, then sterilize them with damp heat and high pressure (121°C) for 25 minutes, and then put them on the ultra-clean bench while they are still hot and put them into sterile petri dishes, and then use them after cooling; For the inclined plane of the test tube, heat it to dissolve the agar and adjust the volume to the prepared amount again, then put it into the test ...

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Abstract

The invention belongs to the field of edible fungi cultivation, and particularly relates to a direct regeneration culture method for tremella aurantia fruiting bodies. Tissue blocks of the tremella aurantia fruiting bodies are cultured individually, or are cultured together with exogenous stereum hirsutum, and thereby, the tissue blocks of the small tremella aurantia fruiting bodies directly growinto large tremella aurantia fruiting bodies. The culture method can improve the success rate of making of a tremella aurantia strain and the inoculation success rate of tremella aurantia cultivationbags, shortens the culture period, and is beneficial to the modernized and industrialized cultivation of tremella aurantia.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a method for directly regenerating fruiting bodies of Auricularia auriculae. Background technique [0002] Existing saprophytic edible fungus cultivation all is to form the pure culture of mycelium (solid or liquid strain) by sporocarp or mycelium culture, transfers with the pure culture of mycelium (solid or liquid strain) again Cultivate the mycelium on the compost, then induce the mycelium to form the fruiting body primordium, and finally cultivate to form the fruiting body (product). Shiitake [1,2,3,4] , Flammulina velutipes [5,6,7] Represented; grass rot fungus is Agaricus bisporus [8] ,straw mushroom [9,10,11] As a representative, the specific process such as figure 1 shown. [0003] The existing parasitic bacteria (white fungus and golden ear) culture technology is: inoculation of mixed strains or two kinds of pure strains after mixing, first cult...

Claims

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Application Information

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IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 李荣春杨林雷
Owner 云南菌视界生物科技有限公司
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