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Method for preparing ginkgolide B by using stereum hirsutum thalli as catalyst

A technology of Basilis pilosa and ginkgolide, which is applied in the field of bioengineering and can solve problems such as difficult large-scale production of ginkgolide

Active Publication Date: 2013-12-18
BEIJING LYUKE TIANCHENG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And there are only nine articles about the preparation of ginkgolide B (a new method for extracting ginkgolide B from ginkgo biloba or ginkgo leaf extract, application number 200510063407.X; a kind of separation of ginkgolide from ginkgolide mixture The method of B, application number: CN201010608847.X, etc.), the ginkgolides contain ginkgolides A, B, C, M and other homologues in the ginkgolides, these patents adopt separation technology to separate ginkgolides from the ginkgolide mixture B, it is difficult to produce ginkgolides in large quantities in industry B

Method used

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  • Method for preparing ginkgolide B by using stereum hirsutum thalli as catalyst

Examples

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Effect test

Embodiment 1

[0017] Preparation of Test Tube Strains

[0018] Preparation of Potato Dextrose Agar Medium: Weigh 200g of potatoes, cut them into slices, add 1000mL of water, boil for 30 minutes, filter, dilute the filtrate to 1000mL, add 20g of agar, heat until the agar is completely melted, and pack into 18×200mm test tubes, 15mL medium per tube, sterilized at 120°C for 30 minutes, cooled to 50°C, placed on an inclined plane, the length of the inclined plane was half the length of the test tube; Cut the strain into 4×4mm small pieces, pick a small piece and transfer it to a test tube containing potato dextrose agar medium, culture it at 20°C for 20 days to obtain the strain on the slant of the test tube, and store the slant of the test tube at 4°C for later use;

[0019] Preparation of Fermentation Broth Containing Ginkgolide B

[0020] 1. Preparation of strains in liquid shake flasks

[0021] The formula of liquid shake flask culture medium is (unit is g / L) glucose 5, corn flour 5, pept...

Embodiment 4

[0037] Embodiment 4 contains the preparation of the fermented liquid of ginkgolide B

[0038] 1. Production of strains in shake flasks

[0039] The formula of shake flask culture medium is (unit is g / L): glucose 40, corn flour 25, peptone 10, KH 2 PO 4 6. MgSO 4 4. The initial pH value is 8.0. A total of 2000mL was prepared, divided into 250mL Erlenmeyer flasks (10 bottles in total), 150mL per bottle, and sterilized at 140°C for 20 minutes. After cooling, cut the slant strains of the test tube of Basidiomyces pilosa isolated from the fruiting body of Auricularia tibetanus into small pieces of 4×4mm in size, pick 4 pieces and transfer them to the Erlenmeyer flask containing the shake flask culture medium , placed on a shaker at 30°C and 150 rpm for 46 hours.

[0040] 2. Preparation of seed tank strains

[0041] The 50L seed tank is filled with 30L seed culture medium, and the formula of the seed tank culture medium is (unit is g / L): glucose 40, corn flour 25, peptone 10, ...

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Abstract

The invention discloses a method for synthesizing ginkgolide B through converting a ginkgolide mixture by using stereum hirsutum thalli as a catalyst, belonging to the field of biotechnologies. The method comprises the following steps: carrying out amplification culture on stereum hirsutum strains, taking thalli which are obtained through a test tube slant, a liquid shake flask, seed culture and thallus separation as the catalyst, converting ginkgolide into ginkgolide B, and enabling a conversion solution to be subjected to centrifugation, ethyl acetate extraction and anhydrous alcohol crystallization, so as to separate ginkgolide B from fermentation liquor. By using the method, the purity of obtained ginkgolide B is not lower than 99%, and the ginkgolide conversion ratio is higher than 90%.

Description

technical field [0001] The invention relates to the technical field of bioengineering, and relates to a new process for preparing ginkgolide B by transforming ginkgo biloba extracts through liquid culture of bastia pilosa strains. Background technique [0002] Studies have proved that ginkgolide is one of the main active ingredients in Ginkgo biloba, and is a specific and effective platelet activating factor (Platelet activating factor PAF) receptor antagonist. Platelet activating factor is an endogenous phospholipid secreted by platelets and various inflammatory tissues. It is the most effective platelet aggregation inducer found so far, and is closely related to the occurrence and development of many diseases. Ginkgolides, as specific antagonists of PAF receptors, have a wide range of pharmacological effects. Studies have found that ginkgolide B has protective effects on the central nervous system and ischemic injury, anti-shock, anti-allergic, antibacterial and anti-infl...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/645
Inventor 张志才
Owner BEIJING LYUKE TIANCHENG BIO TECH
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