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Isolated culture method and application of stereum hirsutum

A technology for the isolation and cultivation of Bacillus chaetebrae, applied in the direction of microorganism-based methods, applications, and isolation of microorganisms, can solve the problems of large differences in shape and functions, and achieve strong consistency in size, fast growth speed, and texture uniform effect

Inactive Publication Date: 2022-07-12
云南菌视界生物科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Inner ring bacilli and outer ring hyphae and image 3 The conventional ITS molecular classification shown is no difference between the conventional ITS molecular classification, both are Basidiomycetes, but the two are very different in shape and function

Method used

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  • Isolated culture method and application of stereum hirsutum
  • Isolated culture method and application of stereum hirsutum
  • Isolated culture method and application of stereum hirsutum

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Embodiment Construction

[0015] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments.

[0016] The method for isolating and culturing Trichoderma trichomes is as follows:

[0017] (1) The seed source of T. trichomes in the inner circle is obtained from the upper layer material in the original seed bottle of the golden ear fruiting body or the upper layer material of the cultivated seed bag, and the culture material of the above parts is aseptically inoculated in a special medium, and placed in a petri dish. The outer and inner circles of D. trichomes that produce distinct antagonistic lines. The components of the special culture medium are as follows: each liter of water contains 3% water extract of golden ear fruit body, 0.2% yeast extract, 0.6% malt extract, 8% rice flour, and 20% agarose. During the first culturing process, both inner and outer trichomes will be produced on the medium. Therefore, it is necessary to purify...

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Abstract

The method comprises the following steps: taking an upper-layer material in a tremella aurantialba sporocarp stock seed bottle or an upper-layer material in a cultivation seed bag, inoculating the upper-layer material into a special culture medium, and generating an outer ring and an inner ring of the stereum hirsutum with obvious antagonism lines in a culture dish; picking the Stereochaete hirsutum, and then inoculating the Stereochaete hirsutum into a special culture medium for purification culture until a dish is full; the special culture medium comprises the following components in per liter of water: 3% of aqueous extract of tremella aurantialba sporocarp, 0.2% of yeast extract, 0.6% of malt extract, 8% of rice meal and 20% of agarose; the method comprises the following steps: fermenting an inner-ring stereum hirsutum liquid until mycelium pellets are dense, using the mycelium pellets as a tremella aurantialba fruiting body cultivar, inoculating the tremella aurantialba fruiting body cultivar into a tremella aurantialba cultivation bag, and cultivating to obtain the tremella aurantialba fruiting body. The tremella aurantialba sporocarp cultivated species are high in growth speed, high in size consistency, full and round, ruddy and fleshy, uniform in lines and not hollow, and after the cultivated species are inoculated, tremella aurantialba grows well and is robust.

Description

technical field [0001] The present invention relates to an effective method for separating and culturing a special form of Trichoderma trichomes, and the application of the Trichoderma trichomoniasis. Background technique [0002] Trichoderma trichomes is a very special fungus with strong saprophytic ability. It is a miscellaneous fungus in the process of many artificial cultivation of edible fungi. It can grow independently to complete its life history. However, once it establishes a parasitic relationship with aureus During the parasitic stage, Trichoderma trichomes loses the ability to complete the life cycle, and the specific mechanism is still unclear. Up to now, there are few research literatures on D. trichomes. [0003] Trichoderma trichomes is a special form of Trichoderma trichomes, which is produced in a specific medium, which is manifested as sparse, prostrate on the medium near the inoculation block, without aerial growth. Mycelium, weak growth, into gray-whit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02A01G18/20A01G18/40C12R1/645
CPCC12N1/14C12N1/02A01G18/20A01G18/40
Inventor 李梦杰杨林雷沈真辉冯发钧杨绍伟子灵山陆青青
Owner 云南菌视界生物科技有限公司
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