Method for breeding new tremella aurantialba variety through combination of tremella aurantialba asexual spores and stereum hirsutum

A technology of R. trichomes and new varieties, applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problem of inability to adapt to refined management and high-efficiency output, poor disease resistance, and unclear genetic background of strains and other problems to achieve the effect of reproducibility and practicability

Active Publication Date: 2020-12-04
云南菌视界生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the past few decades after the successful artificial cultivation of golden ear, the strains were basically isolated from the fruiting body of wild golden ear, and then used continuously Entities are used for countless generations of transgenerational reproduction, resulting in fewer sources of provenance, degradation of strain production performance after multi-generation vegetative propagation, and strains carrying viruses or miscellaneous bacteria. Background The combination of Auricularia spp. and Bacillus chamomile creates a new combination, a new material for innovative Auricularia breeding, from which new strains or varieties of Auricularia spp. have not been reported in journals or patent documents.
[0004]Currently, the quality of commercial golden ear fungus species is uneven, the genetic background of the strain is unclear, and the production characteristics are unstable. use, resulting in fewer sources of provenance...

Method used

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  • Method for breeding new tremella aurantialba variety through combination of tremella aurantialba asexual spores and stereum hirsutum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0042] 1.1 Collection of golden ear strains from different origins and isolation of asexual spores

[0043]1.1.1 Collection of golden ear fruit bodies from different origins;

[0044] The young fruiting bodies of Auricularia aurantium collected from Lijiang, Gongshan and Weixi were wrapped in absorbent paper and kept sealed at 4°C, and the strains were isolated by tissue separation method within 48 hours. When isolating strains, first sterilize the surface of the fruiting body of Auricularia auriculae, and then take a piece of fruiting body tissue of Auricularia aurantii with a size of 5*5*5mm and inoculate it on the medium of Auricularia aurantii. , sucrose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g. After inoculation, culture in the dark at 20°C. After 25 days of cultivation, the fruiting body tissue pieces of Auricularia aurantii are regenerated into small Auricularia aurantii, which are stored in a refrigerator at 4°C for later use.

[0045] 1.1.2 Isolation of as...

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Abstract

The invention relates to a method for breeding a new tremella aurantialba variety. The method comprises the steps that asexual spores and stereum hirsutum in tremella aurantialba strains from different producing areas are separated, cross combination and collocation are carried out on the asexual spores and stereum hirsutum of the tremella aurantialba from different sources, so that excellent properties of female parents of two species are combined into a combined tremella aurantialba sporocarp strain, then, character stability of the tremella aurantialba is maintained through asexual propagation so as to be applied to production, and therefore new strains and new varieties of the tremella aurantialba are formed. The method provides a feasible way for breeding of tremella aurantialba strains or varieties, and tremella aurantialba recombinant strains with high yield and disease resistance are obtained by using the method to breed the tremella aurantialba strains.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a method for selecting and breeding new varieties of Auricularia aureus by combining asexual spores of Auricularia aureus and Bacillus chaete. Background technique [0002] The prior art describes the biological characteristics of golden ear as follows: golden ear is a kind of parasitic fungus, which is parasitic on Bacillus chamomile. The complex composed of hyphae, the growth of golden ear mycelium depends on the provision of nutrients for it by Bacillus chaete. There are few reports on the research on the selection of golden ear strains, usually involving conventional strain propagation methods, and the description of strain quality only involves the difference between effective strains and invalid strains [1-3] . [0003] In the past few decades after the success of the artificial cultivation technology of golden ear, the strains are basically isolated f...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20A01G18/40
CPCA01G18/00A01G18/20A01G18/40
Inventor 李荣春杨林雷
Owner 云南菌视界生物科技有限公司
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