5results about How to "Reliable preparation method" patented technology

The invention belongs to the technical field of lipopeptide preparation, genetic engineering and biological medicine, and particularly relates to novel double-fat-chain lipopeptide as well as a preparation method and application thereof. According to the invention, a novel artificial gene cluster rhc is successfully constructed through precise editing and recombination of a functional module of a natural non-ribosomal lipopeptide gene cluster, and after heterologous expression of the gene cluster, a brand new double-fat-chain lipopeptide product Rhcolide A can be synthesized; the rhc gene cluster used for preparing the novel double-fat-chain lipopeptide Rhcolide A is the first artificial double-fat-chain lipopeptide synthetic gene cluster constructed through genetic engineering modification. A biological function test verifies and finds that Rhcolide A has excellent ferroptosis sensitizing activity, can be used as a ferroptosis sensitizer to be combined with a ferroptosis inducer to prepare a non-small cell carcinoma resisting drug, provides a brand new drug molecular entity for treatment of non-small cell carcinoma, and has important clinical application value and industrialization prospect.

The invention relates to the technical field of mineral extraction, particularly discloses a method for efficiently extracting barite single mineral in black mudstone rich in organic matters, and solves the technical problems that micron-sized barite in black mudstone cannot be efficiently extracted in the prior art, lattice destruction / isotope fractionation of barite is easily caused in the extraction process, the purity and the recovery rate are low and the like. The purity of the extracted barite single mineral is larger than or equal to 99%, the recovery rate of fine-fraction barite is larger than or equal to 90%, crystal lattices are complete and free of damage, isotope fractionation and component change are avoided, and the high-precision geochemical test requirement can be met; meanwhile, the method is suitable for various sedimentary rocks such as marine / continental black mudstone, shale and siliceous mudstone, and the applicability is wide.

The invention discloses a tanshinone IIA derivative as well as a preparation method and application thereof, and provides a set of systematic chemical synthesis route for overcoming the defects that tanshinone IIA is poor in water solubility and low in bioavailability and the antitumor activity needs to be improved. A series of novel derivatives with various structures and general formula structures and pharmaceutical salts thereof are prepared. The core of the technical scheme is that after a specific functional group is introduced into the derivative, the water solubility and the stability of the compound are remarkably improved, and the derivative particularly shows excellent anti-colorectal cancer activity, can effectively inhibit tumor cell proliferation and tumor growth in an animal model, and does not have obvious toxic or side effects. The invention also provides a pharmaceutical composition containing the derivative and application of the pharmaceutical composition in preparation of medicines for preventing, relieving or treating colorectal cancer, and provides an important candidate compound for developing a high-efficiency and low-toxicity novel anti-colorectal cancer medicine.

This invention provides a biomimetic microfluidic device for simulating the three-dimensional structure of alveoli. The fabrication method includes: preparing an inverse opal membrane; bonding the inverse opal membrane to the bottom of a transwell chamber structure (with the bottom filter membrane removed); bonding the top of the transwell chamber to a perforated plate cover; inserting one end of a gas delivery tube through the perforation in the perforated plate cover into the transwell chamber; and connecting the other end to an injection pump; culturing alveolar epithelial cells and vascular endothelial cells on the front and back sides of the inverse opal membrane, respectively, to obtain the biomimetic microfluidic device. The biomimetic microfluidic device provided by this invention can achieve three-dimensional stretching under the action of a microfluidic injection pump, simulating the periodic stretch-recovery process of human lung respiratory movement in vitro. The fabrication method is simple and reliable.

The specification provides a calcium overload and iron death synergistic photodynamic diagnosis and treatment of responsive nano probes and preparation method. The method is to encapsulate porous iron-based metal nanoparticles (PEG-MOF-Fe) by calcium peroxide (HA-CaO2) nanoparticles, and to load the chemotherapeutic drug doxorubicin (DOX) into the pores to form calcium overload and iron death synergistic photodynamic diagnosis and treatment of responsive nano probes MOF-Fe@DOX-CaO2. With the long circulating effect of PEG, the nano probe is efficiently enriched in the tumor, and the tumor location is accurately determined by fluorescence / magnetic resonance multi-modal imaging. After entering the tumor tissue, CaO 2 Nanoparticles will dissolve in the low pH environment of the tumor, and then realize the DOX and Ca 2+ Site-controlled release. Under the co-catalysis of Ca 2+ , TCPP and Fe 2+ , calcium overload and iron death synergistic photodynamic co-action, the effect of efficient diagnosis and treatment of tumor is achieved.