8 results about "Calcium overload" patented technology

The application relates to the field of nanobiomedical technology, and particularly relates to application of polyethylene glycol modified black phosphorus nanosheets in prevention and treatment of acute liver injury. The polyethylene glycol modified black phosphorus nanosheets not only have a significant active oxygen scavenging capacity, but also have calcium ion chelation and buffering capacity, can simultaneously regulate intracellular oxidation-reduction homeostasis and calcium homeostasis, inhibit intracellular calcium overload, especially mitochondrial calcium overload, maintain mitochondrial function, and further reduce liver cell damage, apoptosis and inflammatory response, and play a liver protection role. The application finds that the black phosphorus nanosheets are used in calcium ion chelation and inhibition of cell calcium overload, and breaks through the limitation that the black phosphorus nanosheets are mainly used as active oxygen scavenging materials in the prior art. The material can also adsorb copper ions and realize radioactivity copper labeling without chelating agent, and can be used for liver enrichment tracing and integrated diagnosis and treatment application. The material has a significant protective effect on paracetamol-induced acute liver injury.

This invention belongs to the field of biomedical nanomaterials and radiation damage drugs, specifically relating to a magnesium-ethylene glycol tetraacetic acid (EGTA) nanomedicine, its preparation method, and its applications. This study proposes using magnesium-EGTA nanomedicine to treat radiation dermatitis. EGTA's chelating ability for calcium ions is significantly higher than that of magnesium ions and other metal ions. Its unique ion selectivity provides an important theoretical basis and application potential for targeting and inhibiting mitochondrial calcium overload in the pathological process of radiation dermatitis. Magnesium ions are cofactors of many enzymes and have anti-inflammatory, collagen synthesis-promoting, and angiogenesis-enhancing effects. This study constructs a Mg-EGTA-based nanomedicine system, utilizing EGTA's stronger chelating ability for calcium ions, thereby achieving targeted regulation of mitochondrial calcium homeostasis through a calcium-magnesium ion intelligent replacement mechanism in response to calcium overload, providing new ideas and methods for the prevention and treatment of radiation dermatitis.

The application provides a metal polyphenol network-wrapped calcium peroxide nanoparticle and a preparation method and application thereof, and belongs to the technical field of tumor treatment. The metal polyphenol network-wrapped calcium peroxide nanoparticle has a protective effect on unstable calcium peroxide nanoparticles; the released metal ions catalyze hydrogen peroxide to generate hydroxyl radicals under acidic conditions, and then kill tumor cells, realize chemical kinetic therapy, can also consume glutathione, enhance the accumulation of active oxygen, and then enhance the anti-tumor effect; when dispersed into an acidic environment, the metal polyphenol network layer thereof can rapidly degrade, expose the calcium peroxide nanoparticles, and then explosively release a large amount of calcium ions and oxygen, which can more effectively activate calcium overload and relieve the tumor hypoxic microenvironment; by loading drugs, the effective accumulation of the drugs in the tumor site is improved, the combined treatment of chemical kinetic therapy and chemotherapy is realized, and thus the anti-tumor immune response is activated, and the anti-tumor effect is improved.

This invention discloses a targeted nanoparticle for calcium overload therapy combined with photothermal therapy, specifically involving a lipid-modified cRGD-targeting, MMP-responsive, and pH-sensitive calcium carbonate nanoparticle loaded with indocyanine green (ICG). This invention uses pH-sensitive calcium carbonate, which can decompose and exfoliate within lysosomes, as the matrix material. It leverages cRGD's specific targeting of tumor cells, the tumor microenvironment-specific high expression of matrix metalloproteinases (MMPs) to induce the release of DPPA-1 within the tumor microenvironment, and the low pH characteristic within lysosomes to achieve a combined anti-tumor effect of phototherapy and immunotherapy.

The present disclosure describes a new native peptide designated herein as Dwarf Open Reading Frame, or DWORF. This peptide enhances the apparent activity of the SERCA pump, is positively inotropic and lusitropic, and therefore is provided as a therapeutic agent for disorders characterized by cytosolic calcium overload.

The invention discloses a separated polypeptide for inducing mitochondrial calcium overload and application of the separated polypeptide. The separated polypeptide has an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The separated polypeptide disclosed by the invention can be combined with NCLX protein, and degradation of the NCLX protein can be promoted through m-AAA protease, so that calcium ions can be prevented from being excreted, and calcium overload in mitochondria is avoided. In addition, the separated polypeptide is obviously superior to the existing calcium release inhibitor CGP37157 in the aspect of inducing mitochondrial calcium overload.

This invention discloses a calcium-chelating polydopamine-polyrotaxane nanoparticle, its preparation method, and its application, comprising the following steps: (1) aging dopamine hydrochloride in an alkaline solution, centrifuging to obtain polydopamine nanoparticles; dispersing cyclodextrin and bis-thiol-terminated polyethylene glycol in water, stirring at 0-5°C to prepare polyrotaxane; (2) reacting the obtained polydopamine nanoparticles with polyrotaxane in an alkaline solution to obtain polydopamine-polyrotaxane nanoparticles; (3) stirring the polydopamine-polyrotaxane nanoparticles with calcium salt in an alkaline solution to obtain calcium-chelating polydopamine-polyrotaxane nanoparticles. The nanoparticles prepared by the above method have long-term stable photothermal conversion capabilities and high calcium loading capacity, and can be used for tumor treatment through photothermal effect synergistic controllable calcium overloading.

The invention discloses bio-based nano composite hydrogel as well as a preparation method and application thereof, and relates to the technical field of biological materials. The nano composite hydrogel comprises a bio-based hydrogel carrier and a functional component loaded in the bio-based hydrogel carrier; the functional components comprise an oxidative stress enhancing functional component, a calcium overload inducing functional component and a targeting substance. The materials are synthesized, loaded and modified by methods such as a chemical reduction method, a stirring synthesis method, an in-situ generation method, an embedding method, a coprecipitation method, chemical crosslinking, a sol-gel method, Van der Waals' force, electrostatic interaction and the like. According to the nano-composite hydrogel, oxidative stress and calcium overload in tumors are jointly triggered and enhanced, tumor cells are directly killed, anti-tumor immune response is effectively activated, the process drives self-strengthening circulation of'treatment-immunization-retreatment ', and therefore the efficient and lasting anti-tumor effect is achieved.