Tremella aurantialba liquid strain inoculation cultivation method

A technology of liquid strains and cultivation methods, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problem of hindering the large-scale promotion of substitute cultivation of golden ear, affecting the yield and biotransformation rate of golden ear, and not realizing factory cultivation for the time being. and other problems, to achieve the effect of easy management and implementation, simple management and implementation, and shortening the cultivation cycle

Pending Publication Date: 2021-01-12
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP +1
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Problems solved by technology

In addition, golden ear is easy to be infected by miscellaneous bacteria in the early stage of the formation of golden ear primordium, which leads to rotten ears and irregular ears, which seriously affects the yield and biotransformation rate of golden ear, hindering the cultivation of golden ear as substitute material large-scale promotion of
[0006] As an important edible and medicinal fungus, go

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  • Tremella aurantialba liquid strain inoculation cultivation method

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preparation example Construction

[0022] The preparation method of the liquid culture medium of golden ear is that the raw materials are dissolved in water according to the proportion and mixed, and then sterilized and cooled to obtain the liquid culture medium of golden ear.

[0023] The preparation method of the auricularia liquid strain of the invention comprises: inoculating the basidiomyces chamois strain into the above-mentioned culture medium and placing it on a shaker at 23-27°C for 4-6 days to obtain the auricularia liquid strain.

[0024] The invention relates to a method for inoculating auricularia liquid strains into ears, which is to inoculate the auricularia liquid strains into cultivation bags or cultivation bottles for dark cultivation, and after mycelium germinates, the auricularia tissue pieces are grafted into the inoculation holes of the cultivation material Or the material surface of the half-bag fungus bag continues to be cultivated in dark until the fruiting, and the fruiting management i...

Embodiment 1

[0033] Take 1g of starch, 30g of glucose, 3g of peptone, 4g of potassium dihydrogen phosphate, 2g of magnesium sulfate, and 1 tablet of VB, stir and dissolve in 1L of water, and divide them into Erlenmeyer flasks, sterilize at 121°C for 20 minutes, and cool to obtain a liquid medium. Each bottle of liquid culture medium was inoculated with Claritas strains, cultured on a shaker at 25° C., and fermented for 5 days to obtain liquid strains.

[0034] Add 60 parts of corn cobs, 10 parts of sawdust, 13 parts of wheat bran, 0.3 parts of gypsum, 0.3 parts of lime, and 0.05 parts of potassium dihydrogen phosphate, add water and stir until the water content is 60%, and then obtain the cultivation material. Put the cultivation material into a bag of 5cm*15cm*55cm mushroom bag and sterilize it with high-pressure steam at 121℃ for 40mi, then take it out and cool it; After inserting the liquid strains of chrysalis, use a coat bag to cover the mouth of the bacterial bag; after the liquid st...

Embodiment 2

[0036] Take 3g of starch, 40g of glucose, 4g of peptone, 5g of potassium dihydrogen phosphate, 2-4g of magnesium sulfate, and 2 tablets of VB, stir and dissolve them in 1L of water, and distribute them in triangular flasks, sterilize at 121°C for 30 minutes, and then cool to obtain liquid culture. base. Each bottle of liquid culture medium was inoculated with Claritas strains, cultured on a shaker at 23° C., and fermented for 5 days to obtain liquid strains.

[0037] Add 70 parts of corncobs, 15 parts of wood chips, 14 parts of wheat bran, 0.5 parts of gypsum, 0.5 parts of lime, and 0.1 parts of potassium dihydrogen phosphate to water and stir until the water content reaches 55% to obtain the cultivation material. Pack the cultivation material into a 5cm*15cm*55cm shiitake mushroom bag and sterilize it with high-pressure steam at a temperature of 121°C for 50mi, then take it out and cool it; After inserting the liquid strains of chrysalis, cover the mouth of the bacterial bag...

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Abstract

The invention discloses an efficient tremella aurantialba liquid strain inoculation cultivation method. The method comprises the following steps of inoculating a stereum hirsutum strain into a culturemedium, and then putting the culture medium into a 23-27 DEG C shaking table for culturing for 4-6 days to obtain a tremella aurantialba liquid strain; and inoculating the tremella aurantialba liquidstrain into a cultivation bag or a cultivation bottle for dark culture, grafting a tremella aurantialba tissue block to a cultivation material inoculation hole or the material surface of a half-bag fungus bag after hyphae germinate, continuously performing dark culture until fruiting, performing fruiting management in an illumination environment until maturity, and picking. According to the method, tremella aurantialba liquid strain cultivation is adopted, the period can be shortened, the tremella aurantialba yield can be effectively guaranteed, the short period, the high tremella aurantialbayield, the low pollution rate, the low sporocarp difference rate, the high quality and the like of tremella aurantialba artificial cultivation are achieved, and therefore high-quality artificial cultivation of tremella aurantialba is achieved. The invention provides a liquid culture medium formula, a cultivation material formula, a liquid strain preparation method and six cultivation management methods.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for inoculating and cultivating golden ear liquid strains. Background technique [0002] golden ear ( Tremella aurantialba Bandoni & M. Zang), belonging to Fungi, Basidiomycota, Tremellomycetes, Tremellales, Tremellaceae, Tremella ( Tremella ). After maturity, the fruiting body is golden yellow ear flap shape, and its shape resembles the brain, also known as brain ear, brain-shaped tremella, etc., as a precious edible and medicinal fungus, golden ear contains a large amount of amino acids, proteins and mineral elements needed by the human body , the unique golden ear polysaccharide, as a current research hotspot, is one of the main active ingredients of golden ear, which has various effects such as lowering blood fat, lowering blood sugar, and enhancing immunity. As early as in the "Compendium of Materia Medica", it has been recorded th...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/50A01G18/20A01G18/00
CPCA01G18/40A01G18/50A01G18/20A01G18/00
Inventor 刘绍雄张俊波华蓉孙达锋罗孝坤刘春丽李建英
Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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