Preparation method and application of stereum hirsutum mycelium polysaccharide

A technology of R. pilosicus and mycelium polysaccharide, applied to pharmaceutical compositions and/or health care products containing the polysaccharide, in the field of R. pilosicus mycelial polysaccharide, which can solve the problem of long cycle and lack of commercial feasibility for extracting active ingredients Sexuality, resource threats, etc.

Active Publication Date: 2013-06-12
BEIJING LYUKE TIANCHENG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the excessive utilization of the wild resources of the golden ear, this resource has formed a great threat
The traditional artificial cultivation method has a long cycle, and it takes several months to form a generation of fruiting bodies
The long-term cultivation process results in the instability of fruiting body components, especially those bioactive substances, making extraction of active ingredients from fruiting bodies less commercially viable

Method used

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  • Preparation method and application of stereum hirsutum mycelium polysaccharide
  • Preparation method and application of stereum hirsutum mycelium polysaccharide
  • Preparation method and application of stereum hirsutum mycelium polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the preparation of basilar pilosa fermented liquid and mycelia polysaccharide

[0027] 1. Use the newly configured potato culture medium (Ge Jian, Wang Zhengxiang, Handbook of Industrial Microbiology Experiment Technology, 1994: P367) to transfer the strains of B. pilosae strains to the slant, cultivate at 27° C., and after the mycelium is covered with the slant, put The strains on the slant were inserted into 250 mL Erlenmeyer flasks (84 flasks in total) containing 60 mL of shaker flask culture medium, and cultured on a shaker at 22° C. and 90 rpm for 24 hours. Wherein the formula of shake flask culture medium is (unit is gram / liter): glucose 5, corn meal 5, peptone 1, KH 2 PO 4 1. MgSO 4 1. The initial pH value is 5.0.

[0028] 2. Put 5000mL of the cultured strain above into a 75L seed tank with 45L of seed culture medium. The total volume of the fermentation liquid in the seed tank is 50L; maintain the tank temperature at 22°C, tank pressure at 0.05M...

experiment example 2

[0032] Experimental example 2: preparation of the culture fluid and mycelia polysaccharide of Basilis pilosa

[0033] 1. Use the newly configured potato culture medium (Zhuge Jian, Wang Zhengxiang, Manual of Industrial Microbiology Experiment Technology, 1994: P367) to transfer the strains of Basilobacter pilosae to the inclined surface, and the culture temperature is 25 ° C. After the mycelium is covered with the inclined surface, The slant strains were inserted into 250 mL Erlenmeyer flasks (total 40 flasks) equipped with 90 mL of shaking flask culture medium, and cultured on a shaker at 25° C. and 120 rpm for 36 hours.

[0034] 2. Put 4000mL of the cultured strain above into a 100L seed tank with 50L of seed culture medium, the total amount of fermentation liquid in the seed tank is 54L; maintain the tank temperature at 25°C, the tank pressure at 0.05MPa, and the stirring speed at 120 rpm , ventilation rate 1:0.5v / v / m, culture time 90 hours.

[0035] 3. Insert the 30L seed...

Embodiment 3

[0042] Embodiment 3: the preparation of basilar pilosa fermented liquid and mycelia polysaccharide

[0043] 1. Use the newly configured PDA medium slant (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technical Manual, 1994: P367) to transfer the bacteria strain of Basilobacter pilosae, culture temperature 30 ° C, after the mycelium is covered with the slant, The slant strains were inserted into 250mL Erlenmeyer flasks (10 flasks in total) equipped with 150mL shake flask culture medium, and cultured on a shaker at 30°C and 150 rpm for 46 hours.

[0044] 2. Put 3000mL of the strain cultured in this liquid shake flask into a 30L seed tank equipped with 50L first-level seed medium, and the total fermented liquid in the seed tank is 31.5L; maintain the tank temperature at 30°C, and the tank pressure at 0.05MPa, The stirring speed is 150 rpm, the ventilation rate is 1:1 v / v / m, and the fermentation time is 90 hours.

[0045] 3. Insert 15L of first-class seed stra...

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Abstract

The invention discloses a preparation method and an application of stereum hirsutum mycelium polysaccharide, relates to the field of biological fermentation and medicines, and particularly relates to a production technology process for extracting mycelium polysaccharide by obtaining mycelium through liquid culture of stereum hirsutum, and blood sugar and blood fat reduction effects of the polysaccharide. Repeated experimental study proves that the mycelium polysaccharide produced by applying the production process can obviously reduce the levels of blood sugar and blood fat in hyperglycemic mice through intraperitoneal injection or oral administration, thus proving that the stereum hirsutum mycelium polysaccharide can be applied to the preparation of medicines for reducing blood sugar and blood fat. The preparation method opens the bran-new approach for further development and utilization of stereum hirsutum and can be used for providing a novel ideal traditional Chinese medicine for the reduction of blood sugar and blood fat.

Description

technical field [0001] The present invention relates to biological fermentation and the field of medicine, specifically the present invention relates to the method for producing the mycelia polysaccharide of Basilis pilosa by cultivating Basilis pilosa, the mycelia polysaccharide of Basilis pilosa obtained by the method, and containing the Pharmaceutical composition and / or health product of polysaccharide. The mycelia polysaccharide of the cheesus pilosae has a drug for preventing and / or treating diabetes, especially it has the effect of lowering blood sugar and blood fat, so that it provides an ideal drug for lowering blood sugar and blood fat. Background technique [0002] Diabetes is a common and frequently-occurring disease among middle-aged and elderly people. The complications caused by diabetes seriously endanger human life and health. The existing western medicine hypoglycemic drugs inevitably have side effects, while most of the natural Chinese medicine hypoglycemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10A61P3/06A23L1/09
Inventor 张志才王永生
Owner BEIJING LYUKE TIANCHENG BIO TECH
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