Preparation method of cultivated strain of tremella aurantialba

The technology of cultivated species and golden ear is applied in the field of preparation of golden ear cultivated species, and can solve the problems of low yield and low ear rate, and achieve the effects of low pollution rate, shortened growth period and fast feeding.

Inactive Publication Date: 2016-03-30
何碧芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention makes Auricularia auricosa and associated pilus Bastasis into cultivars respectively by means of seed production respectively, and solves the low rate of aur

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: (used percentage is percentage by weight)

[0025] 1. Culture of bacteria

[0026] 1) The test-tube culture of the second golden ear yeast-like spore of the golden ear cultivar component: the golden ear yeast-like spore was inoculated on the slant of the improved PDA medium and cultivated for 10 days at 20°C, and the improved PDA medium was 10% potato cooking liquid Add 0.05% yeast powder, 0.05% peptone, 0.05% dipotassium hydrogen phosphate (K2HPO4), 0.05% magnesium sulfate (MgSO4), 2% glucose;

[0027] 2) liquid seed culture of golden ear yeast-like spores of the second golden ear cultivar component: add 100% water in 5% soybean flour, 5% corn flour, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, and stir well Divided into Erlenmeyer flasks, sterilized at 121°C for 45 minutes, cooled to 35°C, inserted into test tubes, and cultured on a shaker at 100 rpm at 20°C for 10 days;

[0028] 3) Shaker culture of the golden ear yeast-like spores o...

Embodiment 2

[0039] 1. Culture of bacteria

[0040] 1) The test-tube seed culture of the second component of the cultivar of Auricularia chinensis: the yeast-like spores of Auricularia aureus inoculated on the slant of the improved PDA medium and cultivated for 7 days at 25°C, and the improved PDA medium was 10% potato cooking liquid plus 0.2% yeast extract , 0.2% peptone, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, 2% glucose;

[0041] 2) Liquid seed culture of the second component of the cultivar aurantiaceae: add water surplus in 8% soybean flour, 8% corn flour, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate, stir evenly and divide into triangular flasks medium, sterilized at 121°C for 30 minutes, cooled to 35°C, inserted into the test tube, and cultured on a shaker at 25°C and 300 rpm for 7 days;

[0042] 3) Shaker cultivation of the second component of the cultivar auricularia: add water surplus in 8% soybean flour, 8% corn flour, 8% oak sawdust, 0.1% di...

Embodiment 3

[0053] 1. Culture of bacteria

[0054] 1) The test-tube seed culture of the second component of the cultivar of Auricularia chinensis: the yeast-like spores of Auricularia aureus were inoculated on the slant of the improved PDA medium and cultivated for 7 days at 28°C, and the improved PDA medium was 10% potato cooking liquid plus 0.2% yeast powder , 0.2% peptone, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, 2% sucrose;

[0055] 2) Liquid seed culture of the second component of the cultivar auricularia: 10% soybean flour, 10% corn flour, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, add the water balance, stir evenly and pack in the triangular flask medium, sterilized at 121°C for 60 minutes, cooled to 35°C, inserted into the test tube, and cultured on a shaker at 300 rpm at 28°C for 3 days;

[0056] 3) Shaker cultivation of the second component of the cultivar auricularia: add water surplus in 10% soybean flour, 10% corn flour, 10% oak sawdust,...

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Abstract

The invention discloses a preparation method of a cultivated strain of tremella aurantialba. The preparation method is characterized by comprising steps as follows: strain culture: solid culture and liquid culture of Stereum hirsutum as a first component of the cultivated strain of the tremella aurantialba; test tube seed culture, liquid seed culture, shaking culture and fermentation tank culture of tremella aurantialba yeast-like conidia as a second component of the cultivated strain of the tremella aurantialba; mixing of the first component and the second component of the cultivated strain of the tremella aurantialba; processes of cut-log cultivation and cultivation in a bag or a bottle. The tremella aurantialba and the companion fungi, namely the Stereum hirsutum, in the cultivation of the tremella aurantialba are cultured respectively, the tremella aurantialba and the companion fungi can be sufficiently and uniformly mixed, so that the yeast-like conidia of the tremella aurantialba can germinate sufficiently, the frequent condition that a large quantity of Stereum hirsutum sporocarps grow on cut-logs in the cultivation of the tremella aurantialba can be prevented, with the application of the strain, the fruiting rate of the cut-log cultivation of the tremella aurantialba is higher than 95%, and the yield is increased by 15%-20%; with the application of the liquid-liquid strain of the tremella aurantialba, the tremella aurantialba and the companion fungi are uniformly mixed, during cultivation in the bag or the bottle, the tremella aurantialba spawn running is fast, the colonization is fast quickly, the pollution rate is low, the fruiting rate is up to 100%, the color change is fast, and the growing period is short.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for preparing a golden ear cultivar of a large fungus with associated bacteria. Background technique [0002] Tremella aurantial (Tremella aurantial ba Bandoniet Zang) is an edible and medicinal fungus of the genus Tremella aurantiaceae Tremellaceae of Basidiomycetes. It is mainly distributed in Southwest my country. Tremella aurantium has been successfully artificially cultivated (including section wood and bag material cultivation). [0003] Because golden ear has poor utilization ability to lignin and cellulose, it needs to rely on Stereumhirsutum (willd: Fr.) S.F.Gray) associated with it to provide nutrition under natural conditions to grow. Because Auricularia hyphae are weaker, in the existing cultivar seed production process, the situation that Bacillus chamomile grows rapidly and Auricularia auricula does not grow often occurs, thus resulting in...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G1/04C12R1/645
CPCC12N1/14A01G18/00
Inventor 何碧芳
Owner 何碧芳
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