Method for simultaneously preparing ginkgolide B and quercetin from stereum hirsutum

A technology of ginkgolide and basilar bacteria, applied in the field of bioengineering, can solve the problems of backward purification and separation methods, low separation efficiency, high production cost, etc., and achieve the effect of overcoming inhibition and simple process

Inactive Publication Date: 2016-08-31
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the prior art, a large amount of ginkgolide B and quercetin are generally obtained by using a simple separation and purification method for ginkgolide B and ...

Method used

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  • Method for simultaneously preparing ginkgolide B and quercetin from stereum hirsutum

Examples

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Effect test

Embodiment 1

[0047] Such as figure 1 As shown, a method for simultaneously preparing ginkgolide B and quercetin by using Bastia crassa specifically includes the following steps:

[0048] (1) Expanded culture in test tubes: Cut the slanted strains of Stereum hirsutum ATCC 24992 (purchased from Beijing Beina Chuanglian Biotechnology Research Institute, the same below) in test tubes into small pieces of 3×3 mm, and inoculate Put a small piece into the culture medium on the slant of the test tube, and cultivate it at 20°C for 15 days to obtain the strains on the slant of the test tube, and store the slant of the test tube at 4°C for later use;

[0049] (2) Liquid shake flask culture: Cut the slanted strains of the test tube prepared in step (1) into small pieces of 3×3 mm, pick 10 pieces and inoculate them into a 250mL Erlenmeyer flask filled with 150mL liquid shake flask culture medium Among them, the Erlenmeyer flask was cultured on a shaker for 18 hours under the conditions of a rotating s...

Embodiment 2

[0066] A method for simultaneously preparing ginkgolide B and quercetin by using Bastia crassa specifically comprises the following steps:

[0067] (1) Test tube expansion culture: Cut the slant bacteria in the test tube of Basiderma roughderma into small pieces of 3×3 mm, inoculate a small piece into the test tube slant medium, and culture at 35°C for 4 days to obtain the test tube slant bacteria kind, the inclined plane of the test tube is stored at 4°C for later use;

[0068] (2) Liquid shake flask culture: Cut the slant strain of the test tube prepared in step (1) into small pieces of 3×3 mm, pick 3 pieces and inoculate them into a 250mL Erlenmeyer flask containing 20mL of liquid shake flask culture medium Among them, the Erlenmeyer flask was cultured on a shaking table for 86 hours under the conditions of a rotating speed of 200 rpm and a temperature of 20°C to produce a liquid shake flask strain; the liquid shake flask culture medium was sterilized at 100°C for 90 minute...

Embodiment 3

[0082] A method for simultaneously preparing ginkgolide B and quercetin by using Bastia crassa specifically comprises the following steps:

[0083] (1) Test tube expansion culture: Cut the slant bacteria in the test tube of Basiderma roughderma into small pieces of 3×3 mm, inoculate a small piece into the test tube slant medium, and culture at 28°C for 10 days to obtain the test tube slant bacteria kind, the inclined plane of the test tube is stored at 4°C for later use;

[0084] (2) Liquid shake flask culture: cut the slanted strains of the test tube prepared in step (1) into small pieces of 3×3 mm, pick 7 pieces and inoculate them into a 250mL Erlenmeyer flask filled with 80mL liquid shake flask culture medium Among them, the Erlenmeyer flask was cultured on a shaking table for 57 hours under the conditions of a rotating speed of 120 rpm and a temperature of 28°C to produce a liquid shake flask strain; the liquid shake flask culture medium was sterilized at 120°C for 260 min...

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Abstract

The invention discloses a method for simultaneously preparing ginkgolide B and quercetin from stereum hirsutum and relates to the technical field of biological engineering. The preparation method includes the steps that fermentation liquor containing ginkgolide B and a mycelium containing quercetin are prepared sequentially through the steps of tube enlarged culture, liquid shake culture, seed tank enlarged culture, solid primary fermentation culture, liquid post-fermentation culture and extraction and separation; ginkgolide B and quercetin crystal are prepared through extraction. Peeled ginkgoes and rice serve as a solid matrix, gingko leaf extracts are added, stereum hirsutum serves as a strain to convert ginkgolide and ginkgetin through solid primary fermentation, then quercetin and ginkgolide B are separated through post-fermentation, large-scale industrial production can be achieved, and ginkgolide B and quercetin in gingkoes can be produced at the same time. The process is simple, the purity of ginkgolide B is 90% or above and the purity of quercetin is 95% or above in the obtained product, and preparation purity is high.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for simultaneously preparing ginkgolide B and quercetin by using Basiderma crude dermatitis. Background technique [0002] At present, studies have proved that ginkgolide is one of the main active ingredients in Ginkgo biloba, and is a specific and effective platelet activating factor (Platelet activating factor) receptor antagonist. Platelet activating factor (PAF) is an endogenous phospholipid secreted by platelets and various inflammatory tissues. It is the most effective inducer of platelet aggregation found so far, and is closely related to the occurrence and development of many diseases. Ginkgolides, as specific antagonists of PAF receptors, have a wide range of pharmacological effects. Studies have found that ginkgolide B has protective effects on the central nervous system and ischemic injury, anti-shock, anti-allergic, antibacterial and anti-inflammato...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12P17/06C07D493/22C07D311/30C12R1/645
CPCC12P17/181C07D311/30C07D493/22C12P17/06
Inventor 张志才李金花樊亚娟胡坤雅
Owner JIANGSU UNIV
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