Inoculated culture method for liquified strain of tremella auramtialba

A culture method, a technology of liquid strains, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems such as inability to promote or expand application, difficult preparation of pure culture of golden ear, and cultivation failure.

Pending Publication Date: 2020-07-10
云南菌视界生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In these mixed culture methods, the ratio of Auricularia aureus to Bacillus chamocifera can be artificially regulated, but the preparation technology of Auricularia aureus pure culture is very difficult
In addition, the establishment mechanism of the parasitic relationship between the pure culture of Auricularia aureus and Bacillus chamocifera has not been studied clearly so far. The cultures of the two bacteria often appear incompatibility. The establishment of a parasitic relationship leads to the failure of cultivation. This kind of technology has poor stability and cannot be promoted or expanded.

Method used

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  • Inoculated culture method for liquified strain of tremella auramtialba
  • Inoculated culture method for liquified strain of tremella auramtialba

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1 strain production

[0041] 1.1 Production of mother species

[0042] 1.1.1 Preparation of mother seed medium

[0043] 1.1.1.1 Mother solid medium formula (1L):

[0044] Potato 200g, sucrose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0045] 1.1.1.2 Formula of stock liquid medium (1L):

[0046] Potato 200g, sucrose 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0047] 1.1.1.3 Preparation method of mother seed medium:

[0048] Peeled potato slices, add about 1L of water and boil for 15 minutes, then filter the filtrate with 4 layers of gauze, accurately weigh each medicine, put it into a container that can be fixed, add the filtrate and set the volume to 1L, stir to make all kinds of medicines fully dissolve and evenly dispersion. For the production of petri dishes, divide them into Erlenmeyer flasks and seal them with a gas-permeable film, and then sterilize them with damp heat and high pressure (121°C) for 25 minutes. ; To make a test tube slope, heat to dissol...

Embodiment 2

[0099] 1 strain production

[0100] 1.1 Production of mother species

[0101] 1.1.1 Preparation of mother seed medium

[0102] 1.1.1.1 Mother solid medium formula (1L):

[0103] Soybean powder 5g, sucrose 20g, agar powder 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0104] 1.1.1.2 Formula of stock liquid medium (1L):

[0105] Soybean powder 5g, sucrose 20g, KH 2 PO 4 4g, MgSO 4 3g.

[0106] 1.1.1.3 Preparation method of mother seed medium:

[0107] Calculate the dosage of each component according to the preparation amount, accurately weigh each drug, put it into a container that can be constant, add water and set the volume to the preparation amount, stir to fully dissolve and evenly disperse each drug. Put the petri dishes into Erlenmeyer flasks and seal them with a gas-permeable film, then sterilize them with damp heat and high pressure (121°C) for 25 minutes, and then put them on the ultra-clean bench while they are still hot and put them into sterile petri dishes, and th...

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Abstract

The invention relates to an inoculated culture method for a liquified strain of tremella auramtialba. A beaten tremella auramtialba fruit body is mixed with a stereum hirsutum liquid strain to preparea liquified strain, or the tremella auramtialba fruit body is put into the stereum hirsutum liquid strain to be directly beaten to prepare the liquified strain. A tremella auramtialba culture is inoculated by using the liquid strain, so that tremella auramtialba regeneration points in the inoculated tremella auramtialba culture can be greatly increased, the inoculation success rate of the tremella auramtialba culture is improved to over 99% and the culture cycle is shortened to 60-65 days.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a method for inoculating and cultivating liquefied fungus species of golden ear. Background technique [0002] The research on the biological characteristics of golden ear thinks that golden ear is a kind of parasitic fungus, which is parasitic on Bacillus chamomile. Body, the growth of golden ear mycelium depends on Bacillus chamomile to provide nutrition for it. Based on the guidance of this principle, the existing golden ear cultivation process [1,2,3,4,5,6,7] Such as figure 1 shown. [0003] At present, in the golden ear culture process, the mixed culture of the host and the parasite is used for inoculation or the mixed cultures of the two are mixed for inoculation. After being inoculated into the culture medium or cultivation bag, the mixture of the two needs to Germinate hyphae, make the hyphae of Auricularia auricula parasitize on Bacillus chamomile...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/40A01G18/66
CPCA01G18/00A01G18/40A01G18/66
Inventor 李荣春杨林雷
Owner 云南菌视界生物科技有限公司
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