A kind of preparation method of ginkgo golden ear fruit wine

A technology of golden ear fruit wine and ginkgo, applied in the biological field, can solve the problems of not representing golden ear medicinal varieties, difficult to obtain golden ear products, difficult to obtain golden ear resources, etc., and achieves improved medicinal value, suitable taste, and easy absorption. Effect

Active Publication Date: 2011-12-21
江苏同源堂生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the powerful decomposition and utilization of carbohydrates such as lignocellulosic bacterium, it is very easy to be in the growth advantage in the growth and development history of golden ear. The traditional vegetative propagation method is often obtained by the cultivation of the mycelium of the hair toughness But it may be mistaken for golden ear hyphae, it is difficult to obtain real golden ear products by submerged fermentation
In view of the particularity of the life history of golden ear, Chinese patents CN101225361 B, CN101831369 A, CN101822373 A and CN101182562 A did not identify the species of golden ear fungus used, so it is difficult to guarantee the effect of the patent implementation
Chinese patent 89100117.4 has reported the cultivation method of golden ear substitute mater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of preparation method of ginkgo golden ear fruit wine of the present invention comprises the following steps:

[0033] (1) Inoculate the slant strain of Auricularia cerevisiae spores into PDA medium (Agricultural Microbiology Center of China Microbiological Culture Collection Management Committee, China Agricultural Strain Catalog, 1991 edition: page 93), culture at 18°C, and culture Time 120 hours;

[0034] (2) Inoculate the slant strains of the host pilus bacilli into the PDA medium (the Agricultural Microbiology Center of the China Microbiological Culture Collection Management Committee, the Chinese Agricultural Strain Catalog, 1991 edition: 93 pages), and the culture temperature is 23 ° C. Culture time 120 hours;

[0035] (3) Transfer the yeast-like spore slant strain obtained in step (1) into a triangular flask equipped with a shaker flask seed medium, at 23°C, with a rotation speed of 50 rpm, and a culture time of 36 hours; The above-mentioned golden ear ...

Embodiment 2

[0044] A kind of preparation method of ginkgo golden ear fruit wine of the present invention comprises the following steps:

[0045] (1) Inoculate the slant strain of Auricularia cerevisiae spores into the PDA medium, cultivate at 28°C, and cultivate for 48 hours;

[0046] (2) Inoculate the slant strain of the host pili basilarus into the PDA medium, cultivate at 28°C, and cultivate for 148 hours;

[0047] (3) Transfer the yeast-like spore slant strain obtained in step (1) into a triangular flask equipped with a shaker flask seed medium, at 28°C, with a rotation speed of 250 rpm, and a culture time of 75 hours; The above-mentioned golden ear yeast-like spore shake flask seed culture medium consists of (unit is g / L): glucose 50, bran 100 (clean liquid obtained by boiling juice and filtering), potassium dihydrogen phosphate 2, magnesium sulfate 2, add water to appropriate volume, with an initial pH of 7.0;

[0048] (4) Transfer the host pilus basilar slant strain obtained in s...

Embodiment 3

[0056] A kind of preparation method of ginkgo golden ear fruit wine of the present invention comprises the following steps:

[0057] (1) Inoculate the slant strain of Auricularia cerevisiae spores into the PDA medium, cultivate at 23°C, and cultivate for 96 hours;

[0058] (2) Inoculate the slant strain of the host pili basilarus into the PDA medium, cultivate at 25°C, and cultivate for 136 hours;

[0059] (3) Transfer the yeast-like spore slant strain obtained in step (1) into a triangular flask equipped with a shaker flask seed medium, at 25°C, with a rotation speed of 150 rpm, and a culture time of 66 hours; The above-mentioned golden ear yeast-like spore shake flask seed culture medium consists of (unit is g / L): glucose 40, bran 75 (clean liquid obtained by boiling juice and filtering), potassium dihydrogen phosphate 1, magnesium sulfate 0.5, add water to appropriate volume, with an initial pH of 6.2;

[0060] (4) Transfer the host pilus slant strain obtained in step (2) i...

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PUM

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Abstract

The invention relates to a method for preparing gingko golden fungus fruit wine, which comprises the following steps that: golden fungus type spore and monoxenous stereum hirsutum parent strains are used for carrying out liquid bottle shaking seed culture of the golden fungus type spores, the seed tank culture of the golden fungus type spores, the bottle shaking seed culture of the autoecious stereum hirsutum, the bottle shaking expansion culture of the autoecious stereum hirsutum and the seed tank culture of the autoecious stereum hirsutum, the seed tank seed of the golden fungus type sporesand the seed tank seed of the autoecious stereum hirsutum are subjected to the mixed fermentation culture, ginkgo starch substrates and wine brewing yeasts are added into the mixed fermentation liquid of golden fungus type spores and the autoecious stereum hirsutum for carrying out post fermentation, the gingko golden fungus fruit wine with golden fungus extracellular polysaccharide, stereum hirsutum terpene, ginkgo flavone and bilobalide b can be obtained, the alcohol volume is higher than 3 percent, the ginkgo phenolic acid is smaller than 1ppm, and the hydrocyanic acid is lower than 0.1microgramme per liter. The gingko golden fungus fruit wine obtained by the method has an anti-radiation health care function.

Description

technical field [0001] The invention belongs to the field of biotechnology and food. Specifically, the invention relates to a preparation method of ginkgo golden ear fruit wine, which is to ferment the ginkgo fruit with the yeast-like spores of the golden ear and the host pili. Ability to remove ginkgo toxic components ginkgoic acid and phenolic substances. The ginkgo golden ear fruit wine obtained by the method has anti-radiation health care function. Background technique [0002] Golden fungus is also known as golden fungus, golden fungus, yellow fungus, golden fungus, gold and silver fungus, etc. Latin scientific name Tremella aurantialba The medicinal use of golden ear was first recorded in Tao Hongjing's "Bielu of Famous Doctors", and the twenty-eighth volume of the dish of "Compendium of Materia Medica" in the Ming Dynasty described its shape, medicinal parts, and sexual and flavor functions. Golden ear is sweet and flat in nature and taste, nourishes yin and moist...

Claims

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Application Information

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IPC IPC(8): C12G3/02A61K36/16A61P35/00A61P17/16A61P37/06C12R1/645C12R1/865
Inventor 刘春卉俞建国马维新
Owner 江苏同源堂生物工程有限公司
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