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Method for intensively extracting red-rooted salvia polyphenol acids using composite enzyme hydrolyzing red-rooted salvia

A technology of salvia polyphenolic acid and compound enzyme hydrolysis, applied in the direction of fermentation, can solve the problems of inability to extract the target substance, large consumption of solvent and energy, low processing capacity, etc., and achieves an environmentally friendly extraction process, shortening extraction time, and saving energy effect

Inactive Publication Date: 2006-12-27
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Among the various extraction processes of salvia polyphenolic acids, the more traditional water decoction method, condensation reflux method, and Soxhlet extraction method all consume too much energy, while supercritical carbon dioxide circulation countercurrent extraction, ultrasonic temperature-controlled extraction, and sealed microwave extraction Auxiliary extraction, dynamic continuous countercurrent extraction, capillary electrophoresis and other methods generally have many problems such as high equipment requirements, large solvent and energy consumption, and low processing capacity, and no matter what method is used, the target substance cannot be effectively and fully extracted. This is undoubtedly a great waste for hundreds of millions of tons of salvia miltiorrhiza raw materials, so it is urgent to design a set of salvianolic acid substances with good efficacy, low cost and high purity, and can be used in large quantities. A new process to greatly increase the extraction rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Moderately pulverize the salvia miltiorrhiza to obtain granules of salvia miltiorrhiza with a particle size of 30 mesh; weigh 100 g, and prepare 1500 ml of acetic acid-sodium acetate buffer solution with a solid-to-liquid ratio of 1 (g): 15 (ml) and a pH of 5.0 As the extractant of salvia miltiorrhiza polyphenolic acid, soak the salvia miltiorrhiza granules in the extractant; then prepare 10ml of compound enzyme solution with a concentration of 0.05g / ml as the broken wall according to the mass ratio of enzyme powder and salvia miltiorrhiza granules at 1:200 Agent, wherein cellulase: hemicellulase: xylanase: pectinase: amylase: lipase: protease=60:25:5:5:2:2:1 (mass ratio); Pour the buffer solution soaked with Danshen granules into a 3L extractor, keep the water bath at a constant temperature of 50°C, and continue to stir for a total of 80 minutes of enzymolysis and reflux integrated high-efficiency extraction; the obtained extract is separated and filtered through a micr...

Embodiment 2

[0011] Take by weighing 600g of salvia miltiorrhiza granules that are crushed to 20 meshes, and prepare 12L, 0.1mol / L, pH of acetic acid-sodium acetate buffer solution as the salvianolic acid-like substance of 5.4 by solid-liquid ratio 1 (g): 20 (ml). extracting agent, and the Danshen granule is soaked in the extracting agent; then according to the mass ratio of the enzyme and the Salvia miltiorrhiza granule is 1: 300, the compound enzyme liquid of 0.02g / ml is prepared as the wall-breaking agent with a concentration of 100ml, wherein cellulase: hemicellulose Sulfase: xylanase: pectinase: amylase=50:30:15:3:2 (mass ratio); the enzyme solution and the buffer solution soaked with Salvia miltiorrhiza granules are poured into a 20L extractor, jacketed water bath Keep the temperature at 40°C and keep stirring for a total of 100min of extraction; the obtained extract is centrifuged at 2000r / min for 20min to obtain 1.2L supernatant; directly spray dry, the conditions are: inlet tempera...

Embodiment 3

[0013] The formula of the compound enzyme liquid wall-breaking agent used is: cellulase: hemicellulase: pectinase=60:35:5 (mass ratio), and the rest are the same as in Example 2.

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Abstract

This invention provides the process of enzymatic enhanced extraction of depsides from salvia miltiorrhiza substance. This process includes comminution, soaking, the preparation of enzyme blends, the blending of material and liquid, extraction, filtering, condensing and dring. This process makes full use of effective wall breaking and improve dissolution principle of enzyme blends, and produces water-soluble extraction powder of salvia miltiorrhiza that with many depsides functional substance. This invention integrates the enzymatic hydrolysis and extraction process, and has many benefits such as effective, mild, saving and environment friendly. The extraction rate of depsides from salvia miltiorrhiza is increased significently, reaches 9.5% that increased 3.5% compared with traditional water extraction. The temperature reduced from 100DEG C to 50DEG C, and time for extraction reduced for 1 hour. At the same time it avoids the Polyose Material Gelatinization and oxidation of Depsides such as danshensu. This process is innocuity and harmless. The products can be used as functional substance or medicine.

Description

technical field [0001] The invention relates to a method for intensively extracting salvianolic acid substances by using enzymatic hydrolysis technology, which belongs to the technology of obtaining natural products through biological enzymes. Background technique [0002] Salvia miltiorrhiza has the effects of dispelling blood stasis, relieving pain, promoting blood circulation and promoting menstruation. It is a special drug for clinical treatment of coronary heart disease and angina pectoris. The total annual consumption in the country reaches 100 million tons. There are more than ten kinds of salvianolic acids whose structures have been determined, such as: danshensu, caffeic acid, protocatechuic aldehyde, and salvianolic acid (salvianolic acid) A, B, C, D, E, F, G, H , I, J, salvianic acid B, C, rosmarinic acid, lithospermic acid and isosalvianolic acid C (isosalvianolic acid), etc., wherein Danshensu is D-(+)-β- 3,4-Dihydroxyphenyl lactic acid is one of the main water...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00
Inventor 齐崴何志敏
Owner TIANJIN UNIV
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