A fungus belonging to the genus Carbonara and its application in the preparation of fungi-grained wood

A technology of charcoal horn bacteria and fungi, applied in the field of microorganisms, can solve the problems of inability to process and use, excessive inhalation, incomplete wood, etc., and achieves the advantages of large-scale production and popularization and application, reasonable technical route, and operation process. simple effect

Inactive Publication Date: 2016-04-20
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the naturally obtained fungal wood still has the following deficiencies: 1. The raw materials are not easy to obtain, and there is a strong chance, because the natural fungal wood can only be formed in nature when there are bacteria that can form the fungal wood. , so only a small amount of fungal wood can be found in dead branches and fallen trees in a small number of forests; 2. Natural fungal wood takes a long time to form and is affected by many climate and environmental factors, such as dryness, cold, lack of oxygen, strong sunlight, Other microbial interference, etc.; 3. Natural fungal wood is formed under natural conditions, and there is often the problem of excessive infection of certain bacteria on wood-based materials, resulting in serious loss of strength of the material, which cannot be processed and used, or the permeability is significantly increased. , Excessive inhalation of chemicals will be inhaled during post-treatment such as anti-corrosion and flame-retardant, which will double the cost; 4. Natural fungal grain wood is formed under natural conditions, and the size and shape of the wood are unpredictable, and the beautiful lines may be lost during processing 5. Natural fungus wood is formed under natural conditions and is susceptible to insect pests or other fungal infections, making the wood incomplete or forming unsightly decay or discoloration

Method used

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  • A fungus belonging to the genus Carbonara and its application in the preparation of fungi-grained wood
  • A fungus belonging to the genus Carbonara and its application in the preparation of fungi-grained wood
  • A fungus belonging to the genus Carbonara and its application in the preparation of fungi-grained wood

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preparation example Construction

[0062] The preparation method may also include a post-processing step, in which the hyphae on the surface of the fungal wood obtained after inoculation culture are scraped off, washed and dried to a moisture content of 8-10%.

[0063] The composition of PDA liquid medium is calculated in g / L: potato 160~240, glucose 10~20, natural pH.

[0064] The preferred components of the PDA liquid medium are: Potato 200, Glucose 15, pH 6.5~7.0 in g / L.

[0065] The wood-based material in step B is any one or more of logs, solid wood blocks, planks, veneers, wood flakes, sticks, and special-shaped wood products.

[0066] The tree species of the wood-based material in step B can be any tree species.

[0067] The tree species of the wood-based material in step B is preferably selected from the species of Populus in Salicaceae, Alnus in Betaceae, Betula, Prunus in Rosaceae, Balsa in Kapokaceae or Quercus in Fagaceae.

[0068] The tree species of the wood-based material in step B is more pref...

Embodiment 1

[0079] ——Acquisition, identification and preservation of L2Ma of Daldiniachildiae.

[0080] (1) The acquisition and identification of L2Ma of Daldiniachildiae.

[0081] The fungus belonging to the genus Carbonacola according to the present invention is isolated from decayed stumps, fallen wood or dead branches of the genus Cherry. The sample was collected from the broad-leaved forest near Jindian Park in Kunming City, Yunnan Province. A 100g sample of a decayed tree stump of the genus Cherry with black lines in the xylem was collected, together with the semicircular fungal fruiting body on it.

[0082] Cut the collected decayed tree stump samples into small pieces of 0.3~0.5cm×0.3~0.5cm×0.3~0.5cm with a scalpel, soak them in 75% alcohol for 10~15s, and then soak them in 0.1% mercuric chloride for 5 minutes , and then washed 3 times with sterile distilled water. Then place it in the enrichment medium in the dark at 26°C±2°C, and culture it statically for 10 days until the hyp...

Embodiment 2

[0094] - Preparation of fungal wood from Daldiniachildiae L2Ma (liquid method).

[0095] (1) Preparation of fungal grain wood.

[0096] The L2Ma strain Daldiniachildiae of the present invention, CGMCCNo: 5954, was inoculated on PDA liquid medium, and cultured on a dark shaker at 26°C±2°C for 9 days to form mycelium with a diameter of 0.1-3cm and the suspension of mycelium spores liquid.

[0097] The composition of PDA liquid medium is calculated in g / L: potato 200, glucose 17, pH 6.5~7.0.

[0098]Then, the Southwestern birch log to be inoculated was sawed into square pieces of 2cm×2cm×2cm (12 pieces in total), and they were placed in culture bottles with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture bottle to cover the wood block, add a small amount of water to moisten the wood block and vermiculite, but no excess water overflows, cover the bottle cap, and place it in a high-pressure steam sterilizer at 121 ° C. Sterilize for 40 ...

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Abstract

The invention discloses Daldinia childiae L2Ma and application thereof in preparation of bacterium grain wood, belonging to the technical field of microorganisms. The strain is collected in China General Microbiological Culture Collection Center; the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing City; and the collection number is CGMCC No:5954. The strain has excellent impregnation capability for a wood-based material, the impregnation time is short, and the impregnation depth is large; beautiful colors and grains can be formed on the surface of the material; and the physical mechanical strength is not influenced. A method for preparing a bacterium grain wood by using the strain comprises the steps of strain activation, expanding propagation and inoculation culture; and the method comprises the following specific steps: inoculating the strain on an activation and expanding propagation culture medium, performing activation and expanding propagation at 22-30 DEG C for 7-15 days, sterilizing the wood-based material, enabling the wood-based material to be sufficiently in contact with the strain subjected to activation and expanding propagation, and culturing at 22-30 DEG C for 6-8 weeks. According to the invention, the method is reasonable in process and simple to operate; and the prepared bacterium grain wood has favorable decoration effect and has no influence on the physical mechanical property of the base material, thereby being beneficial to large-scale production and popularization.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a fungus strain of the genus Anthroporum which has a short infection time and large depth, beautiful color and texture, and has no influence on the physical and mechanical strength of wood-based materials and its use in the preparation of fungus-grained wood. Applications. Background technique [0002] The fungal discoloration of wood has long been regarded as a defect of wood, which reduces the value of wood. In fact, fungal discoloration, such as lines and stains, has natural and unique characteristics. When the decay is not serious A wonderful touch. If this kind of wood with natural lines or colors is used to make handicrafts, such as vases, earrings, pens, wood veneers, etc., the added value of wood can be increased. The fungus wood with natural patterns and colors will be popular in the market, especially in the decoration, decoration and handicraft markets. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H15/00B27K5/00
Inventor 邱坚何海珊罗蓓伍建玲伍建榕甘昌涛
Owner SOUTHWEST FORESTRY UNIVERSITY
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