Extraction and Content Determination of Apigenin and Lutein in Chrysanthemum Chrysanthemum

Abstract

The invention discloses a method for extracting and measuring content of apigenin and xanthophyll in chrysanthemum morifolium ramat. The method is characterized by comprising the following steps: drying the chrysanthemum morifolium ramat, grinding, adding the crushed chrysanthemum morifolium ramat into an organic solvent for extraction according to a material-liquid ratio of (1:150)-(1:20), repeatedly extracting for 1-5 times, filtering extracting solution, and performing vacuum concentration until the solution evaporate to be dry, so as to obtain the apigenin and the xanthophyll. The measurement method adopts a high performance liquid chromatography. The operation is simple, the content of two substances, such as the apigenin and the xanthophylls, in the chrysanthemum morifolium ramat can be simultaneously extracted and measured, the complexity of respective extraction and measurement is reduced, the loss of active substances is reduced to the greatest degree, and a theoretical basis is provided for further popularization and deep processing of related chrysanthemum morifolium ramat products.

Description

technical field

[0001] The invention relates to the category of substance content determination, in particular to a method for simultaneously extracting apigenin and lutein from chrysanthemum chrysanthemum and performing content determination. Background technique

[0002] Hangbaiju, also known as chamomile, white chrysanthemum, tea chrysanthemum, medicine chrysanthemum, is a good product in chamomile. Chrysanthemum chrysanthemum is mainly produced in Tongxiang, Zhejiang. It is one of the "Eight Flavors of Zhejiang" authentic medicinal materials, and it is also a dual-use resource for medicine and food newly approved by the Ministry of Health. Chrysanthemum chrysanthemum has high medicinal value and the effect of prolonging life. "Bu Nong Shu" contains "chamomile is sweet and warm, and it is most beneficial to take it for a long time". , treating head wind and heat tonifying", shows the effect of Chrysanthemum chrysanthemum. Regular drinking can enhance the resistance of ...

Examples

Embodiment 1

[0020] Dry Chrysanthemum chrysanthemum in an incubator at 20°C, crush it, take 1g Chrysanthemum chrysanthemum and extract it in 50mL of 95% alcohol for 120 minutes, repeat the extraction 3 times, filter and concentrate the extract to dryness under reduced pressure, and dilute to 10mL with methanol , detected by HPLC.

[0021] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel was used as filler, methanol-water-acetonitrile was used as mobile phase, and the detection wavelength was 328nm for apigenin and 444nm for lutein;

[0022] Preparation of reference substance solution: Accurately weigh apigenin and lutein reference substances, place them in a brown bottle, add methanol to make a solution containing 10ug per 1mL, as the reference substance solution;

[0023] Preparation of the test solution: After the extract was concentrated and evaporated to dryness, the volume was fixed to 10 mL with methanol to obtain the test solution;

[...

Embodiment 2

[0026] Dry the Chrysanthemum chrysanthemum in a 100°C incubator, crush it, take 2g of Chrysanthemum chrysanthemum and extract it in 50mL of acetone for 30 minutes, repeat the extraction twice, filter and concentrate the extract to dryness under reduced pressure, and dilute to 100mL with methanol. Detection by HPLC.

[0027] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel was used as filler, methanol-water-acetonitrile was used as mobile phase, and the detection wavelength was 328nm for apigenin and 444nm for lutein;

[0028] Preparation of reference substance solution: Accurately weigh apigenin and lutein reference substances, place them in a brown bottle, add methanol to make a solution containing 20ug per 1mL, as the reference substance solution;

[0029] Preparation of the test solution: After the extract was concentrated and evaporated to dryness, the volume was fixed to 50mL with methanol to obtain the test solution;

[003...

Embodiment 3

[0032] Dry the Chrysanthemum chrysanthemum in a 50°C incubator, crush it, take 1g of Chrysanthemum chrysanthemum and extract it in 30mL of acetone for 60 minutes, repeat the extraction once, filter and concentrate the extract to dryness under reduced pressure, and dilute to 10mL with methanol. Detection by HPLC.

[0033] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel was used as filler, methanol-water-acetonitrile was used as mobile phase, and the detection wavelength was 328nm for apigenin and 444nm for lutein;

[0034] Preparation of reference substance solution: Accurately weigh apigenin and lutein reference substances, place them in a brown bottle, add methanol to make a solution containing 50ug per 1mL, as the reference substance solution;

[0035] Preparation of the test solution: After the extract was concentrated and evaporated to dryness, the volume was fixed to 25mL with methanol to obtain the test solution;

[0036] ...

technical field

[0001] The invention relates to the category of substance content determination, in particular to a method for simultaneously extracting apigenin and lutein from chrysanthemum chrysanthemum and performing content determination. Background technique

[0002] Hangbaiju, also known as chamomile, white chrysanthemum, tea chrysanthemum, medicine chrysanthemum, is a good product in chamomile. Chrysanthemum chrysanthemum is mainly produced in Tongxiang, Zhejiang. It is one of the "Eight Flavors of Zhejiang" authentic medicinal materials, and it is also a dual-use resource for medicine and food newly approved by the Ministry of Health. Chrysanthemum chrysanthemum has high medicinal value and the effect of prolonging life. "Bu Nong Shu" contains "chamomile is sweet and warm, and it is most beneficial to take it for a long time". , treating head wind and heat tonifying", shows the effect of Chrysanthemum chrysanthemum. Regular drinking can enhance the resistance of ...

Examples

Embodiment 1

[0020] Dry Chrysanthemum chrysanthemum in an incubator at 20°C, crush it, take 1g Chrysanthemum chrysanthemum and extract it in 50mL of 95% alcohol for 120 minutes, repeat the extraction 3 times, filter and concentrate the extract to dryness under reduced pressure, and dilute to 10mL with methanol , detected by HPLC.

[0021] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel was used as filler, methanol-water-acetonitrile was used as mobile phase, and the detection wavelength was 328nm for apigenin and 444nm for lutein;

[0022] Preparation of reference substance solution: Accurately weigh apigenin and lutein reference substances, place them in a brown bottle, add methanol to make a solution containing 10ug per 1mL, as the reference substance solution;

[0023] Preparation of the test solution: After the extract was concentrated and evaporated to dryness, the volume was fixed to 10 mL with methanol to obtain the test solution;

[...

Embodiment 2

[0026] Dry the Chrysanthemum chrysanthemum in a 100°C incubator, crush it, take 2g of Chrysanthemum chrysanthemum and extract it in 50mL of acetone for 30 minutes, repeat the extraction twice, filter and concentrate the extract to dryness under reduced pressure, and dilute to 100mL with methanol. Detection by HPLC.

[0027] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel was used as filler, methanol-water-acetonitrile was used as mobile phase, and the detection wavelength was 328nm for apigenin and 444nm for lutein;

[0028] Preparation of reference substance solution: Accurately weigh apigenin and lutein reference substances, place them in a brown bottle, add methanol to make a solution containing 20ug per 1mL, as the reference substance solution;

[0029] Preparation of the test solution: After the extract was concentrated and evaporated to dryness, the volume was fixed to 50mL with methanol to obtain the test solution;

[003...

Embodiment 3

[0032] Dry the Chrysanthemum chrysanthemum in a 50°C incubator, crush it, take 1g of Chrysanthemum chrysanthemum and extract it in 30mL of acetone for 60 minutes, repeat the extraction once, filter and concentrate the extract to dryness under reduced pressure, and dilute to 10mL with methanol. Detection by HPLC.

[0033] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel was used as filler, methanol-water-acetonitrile was used as mobile phase, and the detection wavelength was 328nm for apigenin and 444nm for lutein;

[0034] Preparation of reference substance solution: Accurately weigh apigenin and lutein reference substances, place them in a brown bottle, add methanol to make a solution containing 50ug per 1mL, as the reference substance solution;

[0035] Preparation of the test solution: After the extract was concentrated and evaporated to dryness, the volume was fixed to 25mL with methanol to obtain the test solution;

[0036] ...