Method for intensively extracting red-rooted salvia polyphenol acids using composite enzyme hydrolyzing red-rooted salvia

Abstract

This invention provides the process of enzymatic enhanced extraction of depsides from salvia miltiorrhiza substance. This process includes comminution, soaking, the preparation of enzyme blends, the blending of material and liquid, extraction, filtering, condensing and dring. This process makes full use of effective wall breaking and improve dissolution principle of enzyme blends, and produces water-soluble extraction powder of salvia miltiorrhiza that with many depsides functional substance. This invention integrates the enzymatic hydrolysis and extraction process, and has many benefits suchas effective, mild, saving and environment friendly. The extraction rate of depsides from salvia miltiorrhiza is increased significently, reaches 9.5% that increased 3.5% compared with traditional water extraction. The temperature reduced from 100DEG C to 50DEG C, and time for extraction reduced for 1 hour. At the same time it avoids the Polyose Material Gelatinization and oxidation of Depsides such as danshensu. This process is innocuity and harmless. The products can be used as functional substance or medicine.

Description

technical field [0001] The invention relates to a method for intensively extracting salvianolic acid substances by using enzymatic hydrolysis technology, which belongs to the technology of obtaining natural products through biological enzymes. Background technique [0002] Salvia miltiorrhiza has the effects of dispelling blood stasis, relieving pain, promoting blood circulation and promoting menstruation. It is a special drug for clinical treatment of coronary heart disease and angina pectoris. The total annual consumption in the country reaches 100 million tons. There are more than ten kinds of salvianolic acids whose structures have been determined, such as: danshensu, caffeic acid, protocatechuic aldehyde, and salvianolic acid (salvianolic acid) A, B, C, D, E, F, G, H , I, J, salvianic acid B, C, rosmarinic acid, lithospermic acid and isosalvianolic acid C (isosalvianolic acid), etc., wherein Danshensu is D-(+)-β- 3,4-Dihydroxyphenyl lactic acid is one of the main water...

AI Technical Summary

Problems solved by technology

[0003] Among the various extraction processes of salvia polyphenolic acids, the more traditional water decoction method, condensation reflux method, and Soxhlet extraction method all consume too much energy, while supercritical carbon dioxide circulation countercurrent extraction, ultrasonic temperature-controlled extraction, and sealed microwave extraction Auxiliary extraction, dynamic continuous countercurrent extraction, capillary electrophoresis and other methods generally have many problems such as high equipment requirements, large solvent and energy consumption, and low processing capacity, and no matter what method is used, the target substance cannot be effectively and fully extracted. This is undoubtedly a great waste for hundreds of millions of tons of salvia miltiorrhiza raw materials, so it is urgent to design a set of salvianolic acid substances with good efficacy, low cost and high purity, and can be used in large quantities. A new process to greatly increase the extraction rate