Artificial multiplication method for carpinus tientaiensis
A technology of hornbeam in Tiantai and artificial reproduction, which is applied in the directions of application, cultivation, agriculture, etc., can solve the problems of difficulty in reproduction of hornbeam in Tiantai, and artificial methods are not easy to reproduce successfully, and achieve the effect of expanding the population.
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Embodiment 1
[0060] Embodiment 1: In the last ten days of October, pick the mature seeds of Hornbeam tiantai, choose suitable mother tree materials when picking seeds, and take into account the characteristics of more forest trees and better seed quality. The age of the tree is more than 50 years, and the height of the tree is 10. ~15m, DBH 15~80 cm, and store the seeds in a ventilated and dry environment at 20°C for one month. Take 500 mature seeds, wash away the empty seeds, and carry out the germination experiment on 405 full seeds. The seeds to be germinated are sterilized with sodium hypochlorite with a mass fraction of 10% for 30 minutes; the seeds are soaked in distilled water at 25°C for 48 hours; Soak in 50mg / L NAA for 24 hours, then put it into GA with a mass fraction of 150mg / L 3Soak in medium for 48 hours; then rinse with distilled water and put the seeds into germination pots. Put the germination pot into a light incubator to germinate, set a constant temperature of 25°C, lig...
Embodiment 2
[0061] Embodiment 2: in late October, pick 500 mature seeds of hornbeam tiantai, wash away the empty seeds, and carry out germination experiment with full 403 seeds, its storage method and breaking dormancy method are the same as embodiment 1, the difference It is to plant the radicle-germinated hornbeam seeds into the planting box filled with soil medium. After the seeds are treated, the first true leaf will grow after 15 days, the second true leaf will grow after 21 days, and the seeds will grow after 23 days. The number of sprouts was 200, and the germination rate was 49.6%. At the beginning of April, when 2 to 3 true leaves grow, the seedlings are moved to multi-span film greenhouses. The daytime temperature is 20-26°C, the nighttime temperature is 10-15°C, the humidity is 72%, and the light is 12000 lx. 70 days after seed treatment 160 seedlings with 4-5 leaves were formed, and the survival rate of transplanted seedlings was 80%.
Embodiment 3
[0062] Embodiment 3: in the last ten days of October, pluck the mature seeds of hornbeam tiantai, 500 grains, wash away the empty grains, and carry out germination experiment with full 390 seeds, its storage method and breaking dormancy method are the same as embodiment 1, the difference What is more is that the roof hornbeam seed that radicle germinates is planted in intelligent sunlight room, is 100cm, the seedling-raising seedbed of thickness 5cm is made into with soil culture medium; Described soil culture medium is alpine deciduous humus soil: river sand=2 : 1, set a constant temperature of 20°C, a humidity of 75%, a light of 3150 lx, a 12h: 12h light-dark cycle, and control the water holding capacity of the matrix at 45%. The cotyledons were unearthed in 5 to 7 days, the first true leaf grew after 14 to 16 days, and the second true leaf grew after 20 to 25 days. On the 28th day, 206 seeds germinated, and the germination rate was 52.84%; A true leaf grows, set the daytime...
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