A kind of tissue culture propagation method of endangered and rare plant quadrangle

Abstract

The invention belongs to the technical field of plant vegetative propagation, and discloses a method for tissue culture propagation of endangered and rare plant quadrangleia. The tissue culture is concentrated in the collection, pretreatment and completion of each treatment stage of the quadrangle wood explant. Through the application of tissue culture technology, the rooting of annual quadrangle tree plants is directly induced for propagation. The annual new branches are used as explants, and the attachments such as dust on the surface of the explants are removed by washing with running water; the cleaning agent disinfects and sterilizes the surface of the explants. The fungicide realizes the disinfection and sterilization of the explants, and the second flushing with sterile water ensures the aseptic environment of the explants after the treatment is completed, and cooperates with the best medium to complete the tissue culture of the quadruped wood. This cultivation method breaks through the artificial breeding and cultivation technology bottleneck of Tetragalus, and realizes the rapid artificial propagation and large-scale production application of relict and endangered desert plants. A large number of tetrahemothia plants were obtained by means of tissue culture vegetative propagation to realize the artificial reproduction of tetrahemothus and provide basic conditions for the continuation and expansion of the population.

Description

A Tissue Culture Propagation Method of Endangered Rare Plant Sihemu technical field The invention belongs to the technical field of plant vegetative propagation, be specifically related to a kind of tissue culture propagation of endangered rare plant Sihemu breeding method. Background technique Sihemu is a unique single species of plant in China, and it is also the most representative ancient remnant rare and endangered plant on the earth. It is known as the "living fossil of plants". Sihemu is a first-class protected plant in Inner Mongolia, and it is listed in the national key protected wild plant list In the first batch, it was designated as a national second-class protected plant, and in the list of national key protected wild plants (the second batch), it was determined as a national protected plant. It is a national first-class protected plant, known as "the giant panda among plants". In the local area of ​​the steppe-like desert zone between the Bushizui Mo...

AI Technical Summary

Problems solved by technology

Tetraphyllum has two types of styles, one type is that the style extends out of the flower before flowering, and the stamens also extend out of the corolla during flowering, but the stamens and pistils are different in length, and the stigma is higher than the anther; the other type is the style before flowering Does not elongate until the style is always lower than the position of the anthers during flowering, which is not conducive to pollination

[0006] Moreover, the seeds of tetrahex wood have high oil content, and many insects and small animals like to eat them, so that a small amount of plump seeds lose the ability to germinate due to being eaten by moths, which will cause the loss of part of the seeds

The seed coat of the tetrahexa seeds will inhibit the germination of the seeds, and the quadranglewood seeds have no post-ripening and vernalization effects. The solid seeds of the year will start to germinate after they fall to the ground. It is difficult for the seedlings to survive in a harsh environment, and it is found that The secretions of the quadrangle wood itself have an impact on its seed germination and seedling survival, and will cause the roots of the seedlings to rot

[0008] It is difficult for the existing asexual propagation techniques commonly used in quadrangle wood to achieve fast and efficient, get rid of the constraints of natural geographical environment and seasons

At present, there are reports on the propagation method of quadrangle wood with cuttings, but the propagation coefficient of cuttings is low and affected by seasons and regions

Tissue culture generally adopts the method of inducing callus to reproduce, but related studies have found that after the callus is transferred many times, there will be chromosomal variation

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