The invention relates to a culture pore plate for
drug concentration screening multi-
cell co-culture and a using method thereof. An upper-layer
cell culture area is arranged on the front surface of acore plate, and a lower-layer
cell culture area and a
concentration gradient generation area are arranged on the back surface; upper-layer
cell culture holes are formed in the upper-layer cell culturearea; lower-layer
cell culture holes are formed in the lower-layer
cell culture area; the upper-layer cell culture holes and the lower-layer cell culture holes correspond to each other in position and are separated by a liquid-permeable membrane; the upstream ends of the
concentration gradient forming channels are communicated with a first liquid inlet and a second liquid inlet, and the downstream ends of the
concentration gradient forming channels are communicated with the lower-layer cell culture holes of the corresponding concentration gradient levels; a liquid outlet is formed in the coreplate; the
tail ends of the lower-layer cell culture holes are communicated with a liquid outlet; and the lower-layer cell culture area and the concentration gradient generation area on the back surface of the core plate are covered and sealed through a liquid sealing film. According to the invention, construction of an in-vitro multi-(more than two)-cell co-
culture model can be realized,
perfusion culture of cells can be realized, and gradient distribution of
drug concentration is realized so as to improve
drug concentration screening efficiency.