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Tissue culture method for Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher

A lampstand and white flower technology, which is applied in the field of plant tissue culture, can solve the problems of no white flower lamps, and achieve the effects of shortening the cultivation time, streamlining the process and saving costs

Inactive Publication Date: 2014-03-26
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no relevant research report on the propagation of Primula albicans by biotechnology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The stem tips of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher were used as explants for initial culture, multiplication and rooting culture, and the stem tips of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher were selected and placed in tap water Add 2 drops of detergent to soak for 2 minutes, rinse with running tap water repeatedly for 10 minutes to remove surface impurities, then disinfect the surface with 75% alcohol, rinse with sterile water 5 times, and disinfect with 0.1% mercury liter solution for 7 minutes , rinsed repeatedly with sterile water for 3 times under sterile conditions, blotted the surface moisture with filter paper, cut off the damaged tissue, and inoculated the shoot tip into cluster bud induction medium MS+1.0mg / l ZT+1.0KT+0.2mg / l NAA, pH 5.8, start culture at 40% humidity and 20°C, inoculate 3 in each bottle; after 7 days, the callus grows and differentiates into cluster buds, and then transfers to the proliferation ...

Embodiment 2

[0027] The stem tips of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher were used as explants for initial culture, multiplication and rooting culture, and the stem tips of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher were selected and placed in tap water Add 3 drops of detergent to soak for 2 minutes, rinse with running tap water repeatedly for 10 minutes to remove surface impurities, then disinfect the surface with 75% alcohol, rinse with sterile water 4 times, and disinfect with 0.1% mercuric solution for 7 minutes , rinsed repeatedly with sterile water for 3 times under sterile conditions, blotted the surface moisture with filter paper, cut off the damaged tissue, and inoculated the shoot tip into cluster bud induction medium MS+1.0mg / l ZT+1.0KT+0.2mg / l NAA, pH 5.8, start the culture, inoculate 4 in each bottle; after 7 days, the callus grows and differentiates into cluster buds, and then transfers to the proliferation and rooting medium MS+1.5m...

Embodiment 3

[0031] The stem tips of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher were used as explants for initial culture, multiplication and rooting culture, and the stem tips of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher were selected and placed in tap water Add 3 drops of detergent to soak for 3 minutes, rinse with running tap water repeatedly for 10 minutes to remove surface impurities, then disinfect the surface with 75% alcohol, rinse with sterile water 5 times, and disinfect with 0.1% mercuric solution for 8 minutes , rinsed repeatedly with sterile water 5 times under sterile conditions, blotted the surface moisture with filter paper, cut off the damaged tissue, and inoculated the shoot tip into cluster bud induction medium MS+1.0mg / l ZT+1.0KT+0.2mg / l NAA, pH 5.8, for starting culture, inoculate 4 in each bottle; after 7 days, the callus grows and differentiates into cluster buds, and then transfers to the proliferation and rooting medium MS+1.5mg...

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Abstract

The invention provides a tissue culture and rapid propagation method for a rare and endangered plant, i.e. Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher. The method comprises the steps of selecting stem apexes of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher as explants and carrying out cluster bud induction, proliferation, rooting culture and test-tube plantlet transplanting. The method has the most important characteristics that during culture, the traditional tissue culture manner is broken through, the process is simplified, a proliferation and rooting integrated culture medium is adopted, and the propagation time is greatly shortened. With the adoption of the method, the situation that about five clusters are generated through the induction of one stem apex in one culture cycle and each cluster has about ten new buds can be achieved, the rooting rate reaches 100%, and the survival rate of transplanting is over 95%, so that the reproductive capacity of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher is greatly improved, and a technical support for the germplasm conservation and scale production of Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher, which is a rare and endangered plant, is provided.

Description

Technical field: [0001] The invention belongs to a plant tissue culture method in plant biotechnology, and in particular relates to a tissue culture rapid propagation method of Primula albicans. Background technique: [0002] Primula beesiana var. leucantha (Balf. f. et Forr.) Fletcher belongs to the genus Primula in the family Primulaceae, with white flowers. It is a variant of Primula beesiana Forr. , mainly distributed in Yulong Snow Mountain, Lijiang, Yunnan and Muli County, Sichuan Province, usually mixed with Xiahongdengtai Primula, occasionally seen in the larger Xiahongdengtai Primula community, more precisely, it is Xiahongdengtai Primula A variant of the Red Lantern Primula. The flowers of primroses are generally pink, purple or yellow, and the white flowers are very rare in the wild and in the cultivated varieties. Therefore, the white-flowered primula is a rare primula in gardening and horticultural applications. According to observations, although the white-fl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 吴之坤田晓玲马永鹏杨映虹
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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