Rare and endangered plant Rhododendron henanense seed seedling artificial breeding method

Abstract

The invention belongs to the technical field of biology, and particularly relates to a rare and endangered plant Rhododendron henanense seed seedling artificial breeding method. The rare and endangered plant Rhododendron henanense seed seedling artificial breeding method comprises the following steps that mature fruits of Rhododendron henanense are collected, and capsules are dissected to obtain mature seeds for later use; a culture dish double-layer filter paper method is adopted, and first-time seedling hardening is carried out after most of the seeds germinate; when the seeds are cultured, the germination temperature is set to be 15-30 DEG C, illumination is set to be 14 h/d, and the illumination intensity is set to be 10000-20000 lx; waiting for about 3 days to see partial germination of the seeds; first-time seedling hardening is carried out on the sixth day to the ninth day after seed germination, and seedlings with two cotyledons grow in 3-4 days; and culturing is continuously carried out. By utilizing the artificial breeding method, the germination rate of the Rhododendron henanense seeds is high, the highest germination rate can exceed 94%, the survival rate of seedlings is high, the bred seedlings grow well, the rare and endangered Rhododendron henanense can be protected, the Rhododendron henanense can be developed and utilized as an ornamental flower variety, and a technical foundation is laid for effectively rescuing the endangered species.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for artificially breeding seedlings of rhododendron lingbao, a rare and endangered plant. Background technique [0002] Lingbao Rhododendron (Rhododendron henanense subsp.lingbaoense Fang) is a perennial evergreen tree belonging to the genus Rhododendron of the family Rhododendron. The Xiaoqinling National Nature Reserve (2000m above sea level) located in the west of Henan Province is a suitable habitat for Rhododendron Lingbao. There are many Rhododendron Lingbao forests in the area. The largest Rhododendron tree has a root diameter of 60cm and a tree height of 5m. Left and right, it is called "King of Rhododendron" by the locals. There are 4 species (including 1 subspecies) of Rhododendronaceae plants distributed in this area, among which Rhododendron Lingbao is a unique species, which grows in the hilltop dwarfs of Laoyachanao, West Changancha and East Changa...

AI Technical Summary

Problems solved by technology

In the process of raising seedlings with traditional seeds, it is found that the seedlings have high requirements on ecological conditions during the construction period, and are very sensitive to changes in physical conditions such as ambient temperature, soil humidity, light, and soil, and it is difficult for seedlings to transform and grow from cotyledons to true leaves. , true leaves tend to turn brownish-yellow, resulting in high seedling mortality

Therefore, the traditional seedling cultivation method can hardly obtain the seedlings of Rhododendron Lingbao seeds that grow normally, so it is necessary to seek artificial breeding technology suitable for this special species in order to change its endangered situation

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