The invention relates to the propagation production of
cane seedlings, and provides a method for removing
cane perennial root
dwarfing germs and rapidly propagating healthy
cane seedlings, which comprises the following steps: little amount of original seeds are treated by hot water, stem tips which are detected to be feminine by the regenerated
plantlet by carrying out speckle
enzyme marker immune test (DB-EIA) is taken as an explant, healthy seedlings are produced by induction culture, secondary propagation culture, root-promoting culture, temporary planting and second-stage or third-stage nursery propagation-extension; furthermore, the disinfection of seed-
cutting tools (cane knives) is paid attention to in the nursery propagation-extension, before the seedlings are used as seeds for large field production after
coming out from nursery, cane stem juice is extracted by sampling, and RSD
virus is detected by using the DB-EIA, thus guaranteeing the providing of qualified seedlings. Themethod has the advantages that:
dwarfing disease pathogenic bacteria of the perennial root of the cane
seedling can be thoroughly removed, annually propagation coefficient can reach as high as more than 10 thousand times, furthermore, the
seedling has no variation, has the advantages of being fast, high-efficient and safe, and is suitable for the industrialized and standardized production of the cane healthy seedlings on scale.