Tissue culture method for one-step seedling formation by using with-leaf petioles of pinellia ternate tissue-culture cluster seedlings

Abstract

The invention provides a tissue culture method for one-step seedling formation by using with-leaf petioles of Jingzhou pinellia ternate tissue-culture cluster seedlings. The method includes the following steps that 1, explants are selected, wherein Jingzhou pinellia ternate seeds are obtained, after disinfection treatment, germination is accelerated to form cluster buds, and the with-leaf petioles are selected as the explants for tissue culture; 2, the explants are inoculated into a seedling formation medium for culture until the seedlings grow to be 4-10 cm, wherein the seedling formation medium includes 0.5-1.2 mg / L of 1 / 2MS + IBA, 0.2-0.8 mg / L of NAA, 0.15-0.25 mg / L of TDZ, 3% of sucrose and 0.7% of agar. The with-leaf petioles of the Jingzhou pinellia ternate tissue-culture cluster seedlings serve as the explants, the special culture medium is adopted, the seedlings can be rapidly formed in only 45 days, the multiplication coefficient and seedling quality of the Jingzhou pinellia ternate seedlings are ensured (the rooting rate is 95% or more and the transplanting survival rate is 100%), and scale production is achieved.

Description

technical field [0001] The invention relates to the technical field of seedling vegetative propagation, in particular to a tissue culture method for one-step growth of seedlings with leafy petioles of clustered seedlings of Pinellia sinensis tissue culture. Background technique [0002] Pinellia belongs to the genus Pinellia of the family Araceae, and its dried tubers are used as medicine. It is an important traditional Chinese medicinal material. The tubers of Pinellia contain chemical components such as volatile oil, nicotine, glutamic acid, and sitosterol. It has the functions of lowering adverse flow and antiemetic, drying dampness and resolving phlegm, eliminating mass and resolving stagnation, etc. The ancient "Shenlong Materia Medica" called Pinellia Shoutian and Shuiyu. It is a perennial herb, mostly excavated in summer and autumn. Its tubers are spherical or oblate, and it likes sandy soil. The exact bulk of traditional Chinese medicinal materials. The original Ji...

AI Technical Summary

Problems solved by technology

However, this case has obvious shortcomings such as slow callus proliferation and low organ differentiation efficiency.

In addition, there are many limiting factors in the selection of explants in this case. The tubers and plant buds used as explants have the problems of incomplete disinfection and high pollution rate; and the seedling time is too long to meet the market demand.

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