Two-step seedling formation method of daphniphyllum calycinum

A technology for planting maple and seedlings, applied in the field of plant tissue culture, can solve problems such as tissue culture of maple trees that have not been seen, and achieve the effects of ensuring the quality of seedlings, ensuring the multiplication coefficient, and realizing large-scale production.

Inactive Publication Date: 2019-01-04
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there has been no report on the tissue culture of Niu Erfeng

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A two-step method for growing seedlings of maple leaves, comprising the following steps:

[0023] (1) Take the young leaves of the newly sprouted maple as explants, and put them in the ultra-clean workbench, cut the edge of the leaves with a scalpel, cut them into small pieces of 1 cm square, and place them in WPM for cultivation medium, cultivated for 35 days under the condition of culture temperature of 23°C, light intensity of 1500lux, and light time of 8h / day to obtain sterile test-tube plantlets; wherein the WPM medium contained 1.0mg / L brassinolide, 0.2 mg / L indolebutyric acid, 0.2mg / L kinetin, 1.0mg / L gibberellin, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH value of the medium is 5.8;

[0024] (2) Rooting culture: Place the robust plants obtained in step (1) in 1 / 2MS rooting medium at a culture temperature of 23°C, a light intensity of 3000 lux, and a light time of 8 hours / day for 30 days to obtain complete Rooted shoots, wherein 1.0mg / L...

Embodiment 2

[0027] A two-step method for growing seedlings of maple leaves, comprising the following steps:

[0028] (1) Take the young leaves of the newly sprouted maple as explants, and put them in the ultra-clean workbench, cut the edge of the leaves with a scalpel, cut them into small pieces of 1 cm square, and place them in WPM for cultivation culture medium, at a culture temperature of 27°C, a light intensity of 1500 lux, and a light time of 10 hours / day to cultivate sterile test-tube plantlets for 35 days; wherein the WPM medium contains 1.5 mg / L of brassinolide, 0.2mg / L indolebutyric acid, 0.2mg / L kinetin, 0.5mg / L gibberellin, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH of the medium The value is 5.8;

[0029] (2) Rooting culture: place the robust plants obtained in step (1) in 1 / 2MS rooting medium at a culture temperature of 27°C, with a light intensity of 3000 lux and a light time of 10 hours / day for 30 days to obtain complete Rooted shoots, wherein 1 / 2...

Embodiment 3

[0032] A two-step method for growing seedlings of maple leaves, comprising the following steps:

[0033] (1) Take the young leaves of the newly sprouted maple as explants, and put them in the ultra-clean workbench, cut the edge of the leaves with a scalpel, cut them into small pieces of 1 cm square, and place them in WPM for cultivation culture medium, at a culture temperature of 25°C, a light intensity of 1500 lux, and a light time of 9 hours / day, cultured for 35 days to obtain sterile test-tube plantlets; wherein the WPM medium contained 2.0 mg / L of brassinolide, 0.2mg / L indolebutyric acid, 0.5mg / L kinetin, 0.5mg / L gibberellin, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH of the medium The value is 5.8;

[0034] (2) Rooting culture: place the strong plants obtained in step (1) in 1 / 2MS rooting medium at a culture temperature of 25°C, light intensity of 3000 lux, and light time of 9 hours / day under the condition of cultivating for 30 days to obtain com...

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PUM

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a two-step seedling formation method of daphniphyllum calycinum. The two-step seedling formation method of daphniphyllum calycinum includes the following steps that 1, newly germinated tender leaves of daphniphyllum calycinum are taken as explants, after sterilization in a conventional way, the explants are placed on a super-clean worktable, the edges of the leaves are cut with an anatomical knife into small pieces of 1 square centimeter, and the small pieces are placed in a modified WPM for 35 days to obtain aseptic test-tube plantlets; 2, rooting culture is conducted; 3, seedling exercising and transplanting are conducted. The multiplication coefficient of the daphniphyllum-calycinum clusterbuds obtained by the culture method reaches 30-40 times, the cluster buds are robust and take roots easily after being inoculated into a rooting medium, and the rooting rate can reach 90% or above.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a two-step seedling-forming method of the eucalyptus. Background technique [0002] Cow ear maple (Daphniphyllum calycinum Benth.) Alias: Bell pork belly, pig belly fruit, pig belly wood, pig belly, pig Luo wood, pig network wood, pig wood, Zhuluzi, goat dung, sheep dung, tiger ear , Crow Gallbladder, Big Ear Comb, Nanling Hunan, Nanling Tiger Skin Nan and Lingnan Tiger Skin Nan. It is a plant of the genus Panacaceae. [0003] The fruit branches, leaves and roots of the Fructus japonica can be used as medicine, and the branches and leaves: expelling wind and relieving pain; detoxifying and reducing swelling. Mainly rheumatic bone pain; sore swelling and poison; bruises and fractures; poisonous snake bites. Roots: heat-clearing and toxic substances removing; promoting blood circulation and removing blood stasis; reducing swelling and relieving pain. Indicates feve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘丽辉劳水兵陈京鸿迟海军
Owner INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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