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A kind of Tissue Culture Breeding Method of Mongolian Simba

A Mongolian coreba, tissue culture technology, applied in the field of plant reproduction, can solve the problems that restrict the standardized planting and production of Mongolian coreba, no reports on artificial planting of coreba, unstable seedling quality, etc., to ensure the quality of seedlings, Achieve large-scale production and easy rooting effect

Active Publication Date: 2017-04-26
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the related research on the artificial planting and seedling breeding technology of Simba
Mongolian coreba is generally propagated by seeds, but in the preliminary seedling breeding work, it was found that there are problems such as low germination rate, irregular germination, and unstable seedling quality in the seed propagation of Mongolian cored bar, which seriously restricts the development of Mongolian cored bark. Standardized cultivation and production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] An example of the tissue culture rapid propagation method of Mongolian bark of the present invention, comprises the following steps:

[0015] (1) Selection and disinfection of explants: Take the plump fruit of Sinba mongolica, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% detergent aqueous solution for 5min, and rinse with linear tap water 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for 8-10min, rinse with sterile water for 3-5 times, and finally use sterile filter paper to remove surface moisture to obtain sterile outer Implant;

[0016] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, wherein in the MS medium, ad...

Embodiment 2

[0019] Another example of the tissue culture rapid propagation method of Mongolian barley of the present invention, comprises the following steps:

[0020] (1) Selection and disinfection of explants: Take the plump fruit of Mongolian sycamore, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% washing essence aqueous solution for 5min, and rinse with linear tap water 15-30min, adding 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for 8-10min, rinsing with sterile water for 3-5 times, and finally using sterile filter paper to remove surface moisture to obtain sterile outer Implant;

[0021] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, where...

Embodiment 3

[0024] Another example of the tissue culture rapid propagation method of Mongolian barley of the present invention comprises the following steps:

[0025] (1) Selection and disinfection of explants: Take the plump fruit of Sinba mongolica, peel off the peel and take out the seeds as explants, soak the explants in 2v / v% detergent aqueous solution for 5min, and rinse with linear tap water 15-30min, adding 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for 8-10min, rinsing with sterile water for 3-5 times, and finally using sterile filter paper to remove surface moisture to obtain sterile outer Implant;

[0026] (2) Induction of the first generation of explants to obtain sterile test-tube plantlets: inoculate the sterile explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 8 Cultivate 45 days under the condition of -10 hours / day to obtain sterile test-tube plantlets, wherein in the ...

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PUM

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Abstract

A cymbaria mongolica maxim tissue culture breeding method comprises the steps of 1, sterilizing cymbaria mongolica maxim seeds which serve as the explants; 2, placing the sterilized explants in an MS minimal medium to obtain sterile test-tube plantlets through induction; 3, placing the sterile test-tube plantlets in an MS breeding medium for test-tube plantlet intermediate propagation to obtain multiple shoots. The growth coefficient of the cymbaria mongolica maxim multiple shoots obtained with the breeding method is 15-20, high-quality cymbaria mongolica maxim seedlings with high survival rate can be provided within a short period of time, and large-scale cymbaria mongolica maxim seedling culture can be effectively achieved.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a method for tissue culture and propagation of Mongolian barley. Background technique [0002] Cymbaria mongolica Maxim is a plant of the genus Scrophulariaceae. Its Mongolian medicine is called "Alatan-Aji". The desert grasslands and mountain grasslands between them are mainly distributed in Inner Mongolia, Heilongjiang, Hebei and other places. The whole herb is used as medicine, which has the functions of drying "Xie Ri Wu Su", clearing away heat, dispelling rheumatism, diuresis, and hemostasis. [0003] At present, the medicinal materials of Xinba on the market mainly come from wild resources. With the improvement of its medicinal value, the market demand for medicinal materials has increased, resulting in excessive mining of Xinba, the reserves of wild resources are also decreasing, and the resources are on the verge of exhaustion. . Although attention to Mongolian medicin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 韦坤华李旻辉徐建平肖冬李林轩缪剑华李翠韦莹王一诺张乐王晓峰
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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