A kind of rapid propagation method of rabbit ear orchid leaf tissue culture

Abstract

The invention relates to a cymbidium lancifolium leaf tissue culture rapid propagation method comprising the following steps: (1) cymbidium lancifolium sterile test-tube seedling young leaves are adopted as explants, and are disinfected; (2) the disinfected explants are subjected to induction culture in an MS basic medium, such that sterile test-tube seedlings are obtained; (3) the sterile test-tube seedlings are subjected to rapid propagation culture in an MS propagation medium, such that cluster buds are obtained; (4) the cluster buds are subjected to seedling-strengthening culture in an MS seedling-strengthening medium, such that robust plants are obtained; (5) the robust plants are subjected to rooting culture in a 1 / 2 MS rooting medium, such that complete seedlings with roots are obtained; and (6) the complete seedlings with roots are subjected to seedling hardening for 4-7 days under an environment with a room temperature of 25 DEG C, and are transplanted into a sterilized coco coir medium. With the method provided by the invention, cymbidium lancifolium high-quality seedlings with high survival rate can be provided within a short time, and a problem of cymbidium lancifolium large-scale seedling raising is effectively solved.

Description

technical field

[0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of rabbit ear orchid leaf tissue culture. Background technique

[0002] Cymbidium lancifolium (Cymbidium lancifolium), also known as broad-leaved orchid, is a semi-epipphytic plant of the orchid family first published in "Exotic Flora" by British scholar Hooke in 1823. It is distributed in Guangdong, Guangxi, Sichuan, and Yunnan. , Guizhou, China Taiwan and Tibet and other provinces, Japan, Vietnam, Nepal, India and Malaysia are also distributed.

[0003] Rabbit ear orchid is a national first-level key protected wild plant. It is one of the Chinese orchids with great ornamental value, with peculiar leaf shape, elegant flowers, quiet fragrance and green and white flower color. In addition, the whole herb can also be used as medicine. It can be harvested throughout the year, washed, cut into sections and used fresh or dried. Carbuncles, sores, furuncles, r...

Examples

Embodiment 1

[0020] An example of the tissue culture rapid propagation method of rabbit ear blue of the present invention, comprises the following steps:

[0021] (1) Selection and disinfection of explants: take the explants of tender leaves of Rabbit ear orchids, remove the leaves, soak in 2% aqueous solution of detergent for 5 minutes, rinse with linear tap water for 15-30 minutes, add 2- 3 drops of Tween-20 in 100mL of 0.1% mercuric chloride for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally, removed surface moisture with sterile filter paper to obtain explants, wherein sterile water was treated with high temperature and high pressure Sterilized distilled water;

[0022] (2) The first generation of explants was induced to obtain sterile test-tube plantlets: the explants obtained in step (1) were placed in the ultra-clean workbench, and the surface of the leaves was scratched with a scalpel and inoculated into the MS induction medium, and the culture temperature was ...

Embodiment 2

[0028] An example of the tissue culture rapid propagation method of rabbit ear blue of the present invention, comprises the following steps:

[0029] (1) Selection and disinfection of explants: take the explants of tender leaves of Rabbit ear orchids, remove the leaves, soak in 2% aqueous solution of detergent for 5 minutes, rinse with linear tap water for 15-30 minutes, add 2- 3 drops of Tween-20 in 100 mL of 0.1% mercuric chloride were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally the surface moisture was removed with sterilized filter paper to obtain explants. Wherein, sterile water is distilled water sterilized by high temperature and high pressure.

[0030] (2) The first generation of explants was induced to obtain sterile test-tube plantlets: the explants obtained in step (1) were placed in the ultra-clean workbench, and the surface of the leaves was scratched with a scalpel and inoculated into the MS induction medium, and the culture ...

Embodiment 3

[0036] An example of the tissue culture rapid propagation method of rabbit ear blue of the present invention, comprises the following steps:

[0037] (1) Selection and disinfection of explants: take the explants of tender leaves of Rabbit ear orchids, remove the leaves, soak in 2% aqueous solution of detergent for 5 minutes, rinse with linear tap water for 15-30 minutes, add 2- 3 drops of Tween-20 in 100 mL of 0.1% mercuric chloride were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally, surface moisture was removed with sterile filter paper to obtain explants. Wherein, sterile water is distilled water sterilized by high temperature and high pressure.

[0038] (2) The first generation of explants was induced to obtain sterile test-tube plantlets: the explants obtained in step (1) were placed in the ultra-clean workbench, and the surface of the leaves was scratched with a scalpel and inoculated into the MS induction medium, and the culture temper...

technical field

[0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of rabbit ear orchid leaf tissue culture. Background technique

[0002] Cymbidium lancifolium (Cymbidium lancifolium), also known as broad-leaved orchid, is a semi-epipphytic plant of the orchid family first published in "Exotic Flora" by British scholar Hooke in 1823. It is distributed in Guangdong, Guangxi, Sichuan, and Yunnan. , Guizhou, China Taiwan and Tibet and other provinces, Japan, Vietnam, Nepal, India and Malaysia are also distributed.

[0003] Rabbit ear orchid is a national first-level key protected wild plant. It is one of the Chinese orchids with great ornamental value, with peculiar leaf shape, elegant flowers, quiet fragrance and green and white flower color. In addition, the whole herb can also be used as medicine. It can be harvested throughout the year, washed, cut into sections and used fresh or dried. Carbuncles, sores, furuncles, r...

Examples

Embodiment 1

[0020] An example of the tissue culture rapid propagation method of rabbit ear blue of the present invention, comprises the following steps:

[0021] (1) Selection and disinfection of explants: take the explants of tender leaves of Rabbit ear orchids, remove the leaves, soak in 2% aqueous solution of detergent for 5 minutes, rinse with linear tap water for 15-30 minutes, add 2- 3 drops of Tween-20 in 100mL of 0.1% mercuric chloride for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally, removed surface moisture with sterile filter paper to obtain explants, wherein sterile water was treated with high temperature and high pressure Sterilized distilled water;

[0022] (2) The first generation of explants was induced to obtain sterile test-tube plantlets: the explants obtained in step (1) were placed in the ultra-clean workbench, and the surface of the leaves was scratched with a scalpel and inoculated into the MS induction medium, and the culture temperature was ...

Embodiment 2

[0028] An example of the tissue culture rapid propagation method of rabbit ear blue of the present invention, comprises the following steps:

[0029] (1) Selection and disinfection of explants: take the explants of tender leaves of Rabbit ear orchids, remove the leaves, soak in 2% aqueous solution of detergent for 5 minutes, rinse with linear tap water for 15-30 minutes, add 2- 3 drops of Tween-20 in 100 mL of 0.1% mercuric chloride were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally the surface moisture was removed with sterilized filter paper to obtain explants. Wherein, sterile water is distilled water sterilized by high temperature and high pressure.

[0030] (2) The first generation of explants was induced to obtain sterile test-tube plantlets: the explants obtained in step (1) were placed in the ultra-clean workbench, and the surface of the leaves was scratched with a scalpel and inoculated into the MS induction medium, and the culture ...

Embodiment 3

[0036] An example of the tissue culture rapid propagation method of rabbit ear blue of the present invention, comprises the following steps:

[0037] (1) Selection and disinfection of explants: take the explants of tender leaves of Rabbit ear orchids, remove the leaves, soak in 2% aqueous solution of detergent for 5 minutes, rinse with linear tap water for 15-30 minutes, add 2- 3 drops of Tween-20 in 100 mL of 0.1% mercuric chloride were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally, surface moisture was removed with sterile filter paper to obtain explants. Wherein, sterile water is distilled water sterilized by high temperature and high pressure.

[0038] (2) The first generation of explants was induced to obtain sterile test-tube plantlets: the explants obtained in step (1) were placed in the ultra-clean workbench, and the surface of the leaves was scratched with a scalpel and inoculated into the MS induction medium, and the culture temper...