The invention discloses a layered seedling growing device used for crop culture. The layered seedling growing device comprises an upper layer frame, a lower layer frame, supporting legs, connecting pipes, threaded connectors, culture grooves, a water bucket, a plug, a soil layer, a rubber ring, a sealing hole, a water flow groove, a turntable, a communicating pipe, a probe, a storage groove, an inserting groove, a water communicating hole, a filter screen, a water adding opening, a lamp holder, a lamp pipe and a water outlet pipe. The layered seedling growing device has the advantages that theupper layer and the lower layer are connected through the connecting pipes, the culture grooves are all formed in the outer walls of the peripheries of the upper layer frame and the lower layer frame, the insides of the culture grooves are used for culturing seedlings, in this way, the double-layer culture grooves can save the space and increase the culturing quantity of the crop seedlings, the inner diameter of the sealing hole inside the rubber ring is smaller than the diameter of the communicating pipe, and when the communicating pipe is inserted into the soil layer to water the seedlings,the rubber ring can cover the communicating pipe to prevent moisture loss in soil. The layered seedling growing device has the advantages that the space is saved, the seedling growing quantity is increased, and the management is facilitated.