Tissue culture propagation expanding method for dendrobium falconeri

A technique for tissue culture and Dendrobium bead, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of reduced resources of Dendrobium bead, not easy to germinate and grow, long growth cycle, etc. Pure, raw material saving effect

Inactive Publication Date: 2018-06-15
康美药业(昆明)种质资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, Dendrobium beaded on the domestic market mainly relies on wild excavation, and a small number of artificial cultivations are generally propagated by division propagation and cuttings. There are few seedlings for propagation, low reproduction rate, and long growth cycle. The probability of artificial pollination is small; due to the constraints of many factors such as few fruit pods, small fruit powder, and difficult germination and growth in the natural environment, the resources of wild Dendrobium beaded are constantly decreasing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for tissue culture and multiplication of dendrobium nobile, comprising:

[0047] Explant selection step: select robust female plant of Dendrobium beaded for 2-4 years, artificially pollinate Dendrobium beaded during flowering in mid-June, allow it to grow fruit pods, and cultivate for 3-4 months. After the fruit pods are ripe, they are picked and stored at 5°C for 2 weeks;

[0048] Explant disinfection steps: take out the fruit pods and put them in a culture bottle, first soak the fruit pods with detergent water for 18 minutes, brush the surface of the seed shell with a brush, and rinse with tap water for 15 minutes, then wash the fruit pods with a concentration of 75% for medical use Soak in alcohol for 50s, rinse with sterile water for 3 times, then use 2% sodium hypochlorite to disinfect the pod shell once for 1 min, then rinse with sterile water for 3 times, and then use 0.2% mercuric chloride for the second time Disinfect for 6 minutes, then rinse 4 times...

Embodiment 2

[0054] A method for tissue culture and multiplication of dendrobium nobile, comprising:

[0055] Explant selection step: select robust female plant of Dendrobium beaded for 2-4 years, artificially pollinate Dendrobium beaded during flowering in mid-June, allow it to grow fruit pods, and cultivate for 3-4 months. After the fruit pods are ripe, they are picked and stored at 3°C ​​for 2 weeks;

[0056] Explant disinfection steps: take out the fruit pods and put them in a culture bottle, first soak the fruit pods with detergent water for 15 minutes, brush the surface of the seed shell with a brush, and rinse with tap water for 10 minutes, then wash the fruit pods with a concentration of 75% for medical use Soak in alcohol for 33s, rinse with sterile water for 3 times, then use 1% sodium hypochlorite to disinfect the pod shell once for 2 minutes, then rinse with sterile water for 3 times, and then use 0.1% mercuric chloride for a second time Disinfect for 8 minutes, then rinse 5 t...

Embodiment 3

[0062] A method for tissue culture and multiplication of dendrobium nobile, comprising:

[0063] Explant selection step: select robust female plant of Dendrobium beaded for 2-4 years, artificially pollinate Dendrobium beaded during flowering in mid-June, allow it to grow fruit pods, and cultivate for 3-4 months. After the fruit pods are ripe, they are picked and stored at 7°C for 3 weeks;

[0064] Explant disinfection steps: take out the fruit pods and put them in a culture bottle, first soak the fruit pods in detergent water for 20 minutes, brush the surface of the seed shell with a brush, and rinse with tap water for 15 minutes, then wash the fruit pods with a concentration of 75% for medical use Soak in alcohol for 60s, rinse with sterile water for 4 times, then use 3% sodium hypochlorite to disinfect the fruit pod shell once for 1 min, then rinse with sterile water for 4 times, and then use 0.3% mercuric chloride for a second time Disinfect for 6 minutes, then rinse 5 tim...

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PUM

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Abstract

The invention discloses a tissue culture propagation expanding method for dendrobium falconeri. The method comprises the steps of an explant selection step, an explant sterilization step, a sowing step, a secondary culture step, a rooting culture step, and a seedling hardening domestication and transplanting step. According to the preparation method provided by the invention, the stability of germplasm is controlled from a female parent source, the breeding speed and breeding quantity of seedlings of the dendrobium falconeri can be effectively improved, the quality of the seedlings can be guaranteed, industrialized production of the high-quality seeds and seedlings of the dendrobium falconeri can be realized, requirements for production can be met, and a current situation of resource scarcity can be relieved.

Description

technical field [0001] The invention relates to the field of tissue culture and seedling cultivation, in particular to a method for tissue culture and propagation of dendrobium beaded dendrobium. Background technique [0002] Dendrobium falconeri Hook. is an orchid, mainly distributed in Bhutan, India, Thailand, Myanmar, Hunan, Guangxi, and Yunnan in China (Shiping, Lvchun, Jinghong, Tengchong, Longling, Yingjiang). Stems hanging, fleshy, thin cylindrical, internodes swollen above the middle, multi-branched, usually swollen into beads at the nodes of the branches; racemes lateral, large flowers, spreading, sepals lavender or water red with dark purple Apex, flowering period 5-6 months. [0003] At present, Dendrobium beaded on the domestic market mainly relies on wild excavation, and a small number of artificial cultivations are generally propagated by division propagation and cuttings. There are few seedlings for propagation, low reproduction rate, and long growth cycle. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许冬瑾严新顾晓波雷连海
Owner 康美药业(昆明)种质资源有限公司
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