Efficient mutation breeding method for apple polyploidy
A technology for mutation breeding and polyploidy, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low polyploidy growth rate, no rooting of clustered buds, loss of high-quality germplasm resources, etc. To achieve the effect of increasing the rooting rate of clump shoots, increasing the rooting rate of clump shoots, and increasing the number of breeding
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Embodiment 1
[0023] Take terminal buds from the top of Danxia fruit tree to obtain 20 explant samples, put them into medium 1 and culture them for 2 days, then culture them in medium 2 for 2 days, and then culture them in medium 3 for 5 weeks , and then subcultured to obtain polyploid seedlings.
[0024] The composition of medium 1 is: MS+30mg / L colchicine+0.3g / L carbon nanotube+0.2g / L brewer's yeast cell wall;
[0025] The composition of medium 2 is: MS+50mg / L colchicine+0.3g / L carbon nanotube+0.2g / L brewer's yeast cell wall;
[0026] The composition of medium 3 is: MS+0.3mg / L IAA+0.3g / L carbon nanotube+0.2g / L brewer's yeast cell wall.
Embodiment 2
[0028] Take terminal buds from the top of Danxia fruit tree to obtain 20 explant samples, put them into medium 1 and culture them for 2 days, then culture them in medium 2 for 2 days, and then culture them in medium 3 for 5 weeks , and then subcultured to obtain polyploid seedlings.
[0029] The composition of medium 1 is: MS+20mg / L colchicine+0.2g / L graphene+0.1g / L brewer's yeast cell wall;
[0030] The composition of medium 2 is: MS+40mg / L colchicine+0.2g / L graphene+0.1g / L brewer's yeast cell wall;
[0031] The composition of medium 3 is: MS+0.2mg / L IAA+0.2g / L graphene+0.1g / L brewer's yeast cell wall.
Embodiment 3
[0033] Take terminal buds from the top of Danxia fruit tree to obtain 20 explant samples, put them into medium 1 and culture them for 2 days, then culture them in medium 2 for 2 days, and then culture them in medium 3 for 5 weeks , and then subcultured to obtain polyploid seedlings.
[0034] The composition of medium 1 is: MS+40mg / L colchicine+0.4g / L carbon nanotube+0.5g / L brewer's yeast cell wall;
[0035] The composition of medium 2 is: MS+80mg / L colchicine+0.4g / L carbon nanotube+0.5g / L brewer's yeast cell wall;
[0036] The composition of medium 3 is: MS+0.5mg / L IAA+0.4g / L carbon nanotube+0.5g / L brewer's yeast cell wall.
[0037] The cluster shoot rooting rate of embodiment 1-3 is counted, and the results are shown in Table 1.
[0038] Table 1
[0039] Example Cluster shoot rooting rate, % Example 1 95 Example 2 90 Example 3 90
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