Efficient mutation breeding method for apple polyploidy

A technology for mutation breeding and polyploidy, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low polyploidy growth rate, no rooting of clustered buds, loss of high-quality germplasm resources, etc. To achieve the effect of increasing the rooting rate of clump shoots, increasing the rooting rate of clump shoots, and increasing the number of breeding

Pending Publication Date: 2022-03-18
POMOLOGY INST SHANXI ACAD OF AGRI SCI
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Problems solved by technology

In the breeding process of triploids, it is easy to appear clustered buds without roots, which can e

Method used

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  • Efficient mutation breeding method for apple polyploidy
  • Efficient mutation breeding method for apple polyploidy

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] Said to the Dan Xia fruit tree to take the top bud, resulting in 20 outer implant samples, in the culture medium 1, for 2 days, and then cultured for 2 days in the medium, then cultured in the medium 3 for 5 weeks. After that, after further cultivation, it was obtained by polyploid seedlings.

[0024] Medium 1 ingredient: ms + 30mg / L autumn water + 0.3 g / l carbon nanotube + 0.2g / L beer yeast cell wall;

[0025] The medium 2 ingredients: MS + 50mg / L autumn water + 0.3 g / L carbon nanotube + 0.2g / L beer yeast cell wall;

[0026] The medium 3 ingredients were: MS + 0.3mg / L IAA + 0.3 g / L carbon nanotubes + 0.2 g / L beer yeast cell wall.

Example Embodiment

[0027] Example 2

[0028] Said to the Dan Xia fruit tree to take the top bud, resulting in 20 outer implant samples, in the culture medium 1, for 2 days, and then cultured for 2 days in the medium, then cultured in the medium 3 for 5 weeks. After that, after further cultivation, it was obtained by polyploid seedlings.

[0029] Medium 1 ingredient: MS + 20 mg / L Autumn water Xortin + 0.2 g / l graphene + 0.1g / L beer yeast cell wall;

[0030] The medium 2 ingredients: MS + 40mg / L autumn water + 0.2 g / l graphene + 0.1g / L beer yeast cell wall;

[0031] The medium 3 ingredients were: MS + 0.2mg / L IAa + 0.2 g / l graphene + 0.1 g / L beer yeast cell wall.

Example Embodiment

[0032] Example 3

[0033] Said to the Dan Xia fruit tree to take the top bud, resulting in 20 outer implant samples, in the culture medium 1, for 2 days, and then cultured for 2 days in the medium, then cultured in the medium 3 for 5 weeks. After that, after further cultivation, it was obtained by polyploid seedlings.

[0034] The medium 1 ingredient: MS + 40mg / L autumn water + 0.4 g / l carbon nanotube + 0.5g / L beer yeast cell wall;

[0035] Medium 2 ingredients: MS + 80mg / L autumn water + 0.4 g / l carbon nanotube + 0.5g / L beer yeast cell wall;

[0036] The medium 3 ingredients were: MS + 0.5 mg / l Iaa + 0.4 g / L carbon nanotube + 0.5 g / L beer yeast cell wall.

[0037] Statistics on the bud binder of Example 1-3, the results are shown in Table 1.

[0038] Table 1

[0039] Example Cluster branch rate,% Example 1 95 Example 2 90 Example 3 90

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Abstract

The invention discloses an efficient mutation breeding method for apple polyploidy, and relates to the technical field of agricultural planting. Comprising the following steps: performing doubling culture on apple explants in a culture medium containing colchicine, a carbon nanomaterial and beer yeast cell walls, and performing tissue culture and subculture in the culture medium containing the carbon nanomaterial and the beer yeast cell walls. According to the cross breeding method, the rooting rate of cluster buds in the apple triploid breeding process can be increased, and then the utilization rate of high-quality germplasm resources is increased.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a high-efficiency mutation breeding method for apple polyploids. Background technique [0002] Apple is a worldwide fruit tree with strong ecological adaptability, high nutritional value, good storability and long supply cycle. At present, the cultivated apple varieties are mainly diploid, and there are a few polyploid. Polyploid fruit trees generally have the advantages of strong growth, thick branches, thick leaves, large fruit, few or no seeds, high yield, and strong adaptability. Therefore, polyploid breeding has always been an important way of fruit tree breeding. Apple polyploidy is mostly triploid. In the breeding process of triploids, it is easy to appear the phenomenon that clustered buds do not take root, and it is very easy to cause the loss of high-quality germplasm resources, and the breeding rate of polyploids is low. Contents of the invention [...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 续海红杨凯窦彦鑫王芸芸金梦晨褚明丽马冬格
Owner POMOLOGY INST SHANXI ACAD OF AGRI SCI
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