Tissue culture seedling raising method for dendrobium transparens

A tissue culture and dendrobium technology, which is applied to the field of tissue culture and seedling raising of Dendrobium aster, can solve the problems such as hindered breeding of Dendrobium aster, difficult to germinate and grow, long growth cycle, etc., and achieves the improvement of the breeding speed and the number of breeding, the pure variety, and the saving. The effect of raw materials

Inactive Publication Date: 2018-06-15
康美药业(昆明)种质资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the existing Dendrobium asteroides has the following defects: the Dendrobium asteroides currently circulating in the domestic market are mainly imported from Myanmar, a small part comes from Guangxi, there is no artificial cultivation, there are few female parents, and the provenance is scarce; Propagation and cuttings are used to reproduce, with fewer seedlings, low reproduction rate, and long growth cycle. Under normal growth conditions, the probability of results without artificial pollination is small; due to the small number of fruit pods and small fruit powder, it is not easy to reproduce in a natural environment. Many factors, such as germination and growth, hinder the breeding of Dendrobium asterium seedlings with high ornamental value.

Method used

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  • Tissue culture seedling raising method for dendrobium transparens
  • Tissue culture seedling raising method for dendrobium transparens
  • Tissue culture seedling raising method for dendrobium transparens

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A method for raising seedlings in tissue culture of Dendrobium asteraceae, comprising:

[0046] Explant selection steps: select the female parent plant of Dendrobium asteroides with a plant age of 3 years, carry out artificial pollination during the flowering period (mid-June) of the female parent plant of Dendrobium asteraceae, after growing fruit pods, continue to cultivate for 3 months, and pick and mature Fruit pods (yellow-green outer seed shell, intact and uncracked) and stored in a refrigerator at 5°C for 2 weeks;

[0047] Fruit pod disinfection steps: take the spare fruit pods, soak them in detergent water for 15 minutes, brush the surface of the seed shells with a brush, and rinse them with tap water for 12 minutes to obtain the first clean fruit pods; put the first clean fruit pods into a clean beaker , moved to the inoculation room, soaked in 75% medical alcohol on the aseptic operating table for 50s, kept stirring with tweezers during the soaking, to ensure ...

Embodiment 2

[0055] The difference between embodiment 2 and embodiment 1 is:

[0056] In the explant selection step: select the female parent plant of Dendrobium asteroides with a plant age of 4 years, after growing fruit pods, continue to cultivate for 4 months, pick mature fruit pods and store them in a refrigerator at 7°C for 2.5 weeks;

[0057] In the fruit pod disinfection step: take the spare fruit pod, soak it in washing powder water for 20 minutes, clean the surface of the seed shell with a brush, and rinse it with tap water for 15 minutes to obtain the first clean fruit pod; put the first clean fruit pod into a clean beaker , moved to the inoculation room, soaked in 75% medical alcohol for 30s on the aseptic operating table, stirred continuously with tweezers during the soaking period to ensure thorough disinfection, and then rinsed with sterile water for 3 times to obtain the second clean fruit pod; The clean pods were disinfected with 2% sodium hypochlorite for 1 min, then rinse...

Embodiment 3

[0066] The difference between embodiment 3 and embodiment 1 is:

[0067] In the explant selection step: select the female parent plant of Dendrobium asteroides with a plant age of 2 years, after growing fruit pods, continue to cultivate for 3.5 months, pick mature fruit pods and store them in a refrigerator at 3°C ​​for 1.5 weeks;

[0068] In the fruit pod disinfection step: take the spare fruit pod, soak it in washing powder water for 15 minutes, clean the surface of the seed shell with a brush, and rinse it with tap water for 10 minutes to obtain the first clean fruit pod; put the first clean fruit pod into a clean beaker , moved to the inoculation room, soaked with 75% medical alcohol for 60s on the aseptic operating table, stirred continuously with tweezers during the soaking period to ensure thorough disinfection, and then rinsed with sterile water for 3 times to obtain the second clean fruit pod; the second clean fruit pod was obtained; The clean fruit pods were disinfec...

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Abstract

The invention discloses a tissue culture seedling raising method for dendrobium transparens. The method comprises the following steps: an explant selection step, a capsule disinfection step, a sowingstep, a secondary culture step, a rooting culture step, a seedling hardening domestication step and a transplanting step. The tissue culture seedling raising method for the dendrobium transparens provided by the invention can effectively increase the breeding speed and breeding quantity of seedlings of the dendrobium transparens, control the stability of germplasm from a female parent source, ensure the quality of the seedlings, realize industrialized production of high-quality seeds and seedlings of the dendrobium transparens, meet production needs, and relieve a current situation of resourcescarcity.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for growing seedlings of Dendrobium asteraceae through tissue culture. Background technique [0002] Dendrobium transparents is an orchid family, mainly distributed in Myanmar, and mainly distributed in Guangxi in China, most of which are imported from Myanmar; the stems of Dendrobium transparents are drooping, slightly fleshy, cylindrical, unbranched, and multi-noded. There are many racemes, which grow to the old stems of deciduous leaves. Each flower stalk usually has 2 flowers, the flower shape is pinkish purple, membranous, and unfolded; the petals are nearly oval, and the lips are pinkish purple in oval shape. The side surrounding the stamen column forms a trumpet shape, and the horn-shaped part is dark purple. The resources of Dendrobium aster are scarce, the flower color is fresh and beautiful, and it has a light fragrance. The flowering period is as long ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/08
CPCA01H4/008A01C1/08A01H4/001
Inventor 许冬瑾严新雷连海杜永魏
Owner 康美药业(昆明)种质资源有限公司
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