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Tissue culture and rapid propagation method of Jin-mei yangtao actinidia fruits

A technology for tissue culture and rapid propagation of kiwifruit, which is applied in the field of tissue culture and rapid propagation of Jinmei kiwifruit varieties, can solve the problems of insufficient seed and seedling sources, low survival rate of cuttings, limited number of grafted scions, etc., and saves time and human resources. The effect of reducing root damage and reducing production costs

Inactive Publication Date: 2019-03-01
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the market mainly adopts grafting and cutting methods to propagate golden plum seedlings. The survival rate of cuttings is low, and the number of grafted scions is limited, so that the large-scale promotion of golden plum faces the problem of insufficient seedling sources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A kind of tissue culture rapid propagation method of kiwifruit golden plum, completes according to the following steps:

[0046] 1. Obtaining sterile vaccines

[0047] At the end of November, cut the annual branches with strong growth and full axillary buds from the golden plum tree, cut them into 30cm long branches, and each section has 5-10 buds. The upper end of the morphology is sealed with a sealing film, and the lower end of the morphology is soaked in 2‰ sucrose aqueous solution Indoor cultivation, keep the ambient temperature at 25±2°C, keep the room clean, replace the water solution every 2-3 days, two weeks later, the axillary buds will sprout, and when the buds grow to 1-2cm, take the young buds on the ultra-clean workbench Put it in a sterilized empty bottle, sterilize the surface with 75% alcohol for 1 minute, pour out the alcohol, add 0.1% mercury liter in mass volume ratio for further disinfection for 6 minutes, and fully shake the mercuric acid solution ...

Embodiment 2

[0058] A kind of tissue culture rapid propagation method of kiwifruit golden plum, completes according to the following steps:

[0059] 1. Obtaining sterile vaccines

[0060] At the end of November, cut the annual branches with strong growth and full axillary buds from the golden plum tree, cut them into 30cm long branches, and each section has 5-10 buds. The upper end of the morphology is sealed with a sealing film, and the lower end of the morphology is soaked in 2‰ sucrose aqueous solution Indoor cultivation, keep the ambient temperature at 25±2°C, keep the room clean, replace the water solution every 2-3 days, two weeks later, the axillary buds will sprout, and when the buds grow to 1-2cm, take the young buds on the ultra-clean workbench Put it in a sterilized empty bottle, sterilize the surface with 75% alcohol for 1 minute, pour out the alcohol, add 0.1% mercury liter in mass volume ratio for further disinfection for 8 minutes, and fully shake the mercuric acid solution ...

Embodiment 3

[0071] A kind of tissue culture rapid propagation method of kiwifruit golden plum, completes according to the following steps:

[0072] 1. Obtaining sterile vaccines

[0073] At the end of November, cut the annual branches with strong growth and full axillary buds from the golden plum tree, cut them into 30cm long branches, and each section has 5-10 buds. The upper end of the morphology is sealed with a sealing film, and the lower end of the morphology is soaked in 2‰ sucrose aqueous solution Indoor cultivation, keep the ambient temperature at 25±2°C, keep the room clean, replace the water solution every 2-3 days, two weeks later, the axillary buds will sprout, and when the buds grow to 1-2cm, take the young buds on the ultra-clean workbench Put it in a sterilized empty bottle, sterilize the surface with 75% alcohol for 1 minute, pour out the alcohol, add 0.1% mercury liter in mass volume ratio for further disinfection for 10 minutes, and fully shake the mercuric acid solution...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method of Jin-mei yangtao actinidia fruits. The method includes the steps: (1) aseptic seedling acquisition; (2) adventitious bud regeneration; (3) adventitious bud proliferation and strong seedling culture; (4) rooting culture; (5) transplanting. The method is characterized by a germination culture medium, a regeneration culture medium, a proliferated strong seedling culture medium, a rooting culture medium and corresponding culture conditions. The four targeted culture media are used for tissue culture of the Jin-mei yangtao actinidia fruits, a large number of Jin-mei yangtao actinidia fruit seeds and seedlings can be rapidly propagated in a short time, seed and seedling quality can be maximally ensured by tissue culture, seed and seedling propagation speed is increased, industrial production of the seeds and seedlings is realized, and the requirements of production for the Jin-mei yangtao actinidia fruit seeds and seedlings are met. Variety seeds and seedlings are directly subjected to tissue culture, a variety cutting orchard is built, the bottleneck of scion grafting in traditional grafting propagation is broken, and production requirements are sufficiently met.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of kiwifruit varieties, in particular to a method for tissue culture and rapid propagation of Jinmei kiwifruit varieties. Background technique [0002] Jinmei is an excellent plant selected from the F1 generation population by crossing 'Jinyan' with Chinese red-fleshed kiwifruit male plants in Wuhan Botanical Garden of the Chinese Academy of Sciences in 2002. After 10 years of progeny identification and regional tests, it was selected and bred. In 2013, it applied for the protection of new agricultural varieties. The fruit is oblong and oval, small at the top and large at the bottom, with an average fruit weight of about 90 grams, greenish-brown peel, and short hairs on the fruit surface. The flesh is yellow or yellow-green, with sweet taste, strong aroma, tender, juicy and good taste. After the fruit is soft and ripe, the average content of soluble solids is 16%, dry matter i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 钟彩虹吕海燕李大卫
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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