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In-vitro culture method for berchemia lineata

A technology of iron-coated gold and solid culture medium, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of few tissue culture seedlings, no reports on iron-coated gold tissue culture, etc., and achieves improved reproduction efficiency. , to ensure the quality of seedlings, the effect of simple methods

Inactive Publication Date: 2015-01-28
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many Rhamnaceae plants that have successfully obtained tissue culture seedlings through tissue culture, and there is no report on the tissue culture of iron-coated gold.

Method used

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  • In-vitro culture method for berchemia lineata
  • In-vitro culture method for berchemia lineata
  • In-vitro culture method for berchemia lineata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 Basic research of the present invention

[0026] 1. Germination of axillary buds

[0027] (1) Effects of plant growth regulators NAA and 6-BA on germination of axillary buds in segmented stems

[0028] See Table 1 for the effects of plant growth regulators NAA and 6-BA on the germination of axillary buds on sectioned stems. With 1 / 2MS as the basic medium, 30 g / L of sucrose and 7 g / L of agar were added to the medium, and the pH was adjusted to 5.8. The culture temperature is (25±1) ℃, the continuous light is 14 h / d, and the light intensity is 1500-2000Lx. (Unless otherwise specified, the same below) After 40 days of cultivation, record the growth quality of the seedlings, and count the germination rate of axillary buds and the rate of defoliation.

[0029] It can be seen from Table 1 that the growth of axillary bud seedlings is not ideal, and the seedlings have different degrees of leaf defoliation after one month of growth. It can be seen that the combi...

Embodiment 2

[0082] Example 2 Cultivation experiment

[0083] Experimental group 1

[0084] Axillary bud germination medium: IBA 0.1 mg / L, 6-BA 0.05 mg / L, hydrolyzed milk protein 500mg / L, 30g / L sucrose, 7g / L agar powder, WPM medium supplemented to 1L, pH adjusted to 5.8.

[0085] Rooting medium: IBA 0.1 mg / L, 6-BA 0.05mg / L, glucose 60g / L, agar powder 7g / L, WPM medium supplemented to 1L, pH adjusted to 5.8.

[0086] Experimental group 2

[0087] Axillary bud germination medium: IBA 0.05 mg / L, 6-BA 0.05 mg / L, 30g / L sucrose, 7g / L agar powder, WPM medium supplemented to 1L, pH adjusted to 5.8.

[0088] Rooting medium: IBA 0.1 mg / L, 6-BA 0.05mg / L, glucose 60g / L, agar powder 7g / L, WPM medium supplemented to 1L, pH adjusted to 5.8.

[0089] Experimental group 3

[0090] Axillary bud germination medium: IBA 0.15 mg / L, 6-BA 0.10 mg / L, hydrolyzed milk protein 500mg / L, 20g / L sucrose, 7g / L agar powder, WPM medium supplemented to 1L, pH adjusted to 5.5.

[0091] Rooting medium: IBA 0.05 mg / L, ...

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Abstract

The invention discloses an in-vitro culture method for berchemia lineata. The in-vitro culture method for the berchemia lineata comprises the following steps: selecting a new tip sprouted in current year on a berchemia lineata plant; performing disinfection processing, and connecting the new tip into an auxiliary bud germinating culture medium for culturing; when an auxiliary bud extends to 3 to 5 cm, cutting the germinated tender tip, transferring the tender tip to a rooting culture medium and performing rooting culture; performing seedling hardening on a test tube plantlet with good root growth. The method disclosed by the invention is simple, quick and high-efficiency; the reproductive efficiency of berchemia lineata seedlings is effectively improved; the seedling quality is ensured. By adopting the method disclosed by the invention, one small berchemia lineata seedling of the current year can be changed into about 8,000 neat seedlings after one year, i.e., a large amount of high-quality seedlings can be reproduced quickly to meet the requirement on the berchemia lineata seedlings of a medicine market and enterprises of herbal tea production and the like. In addition, the method also has the characteristics that the survival rate after the berchemia lineata is cultured in a large field is high; the seedlings grow quickly; roots (medicinal parts) are thick and strong.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an iron-clad gold in vitro culture method. Background technique [0002] Iron-clad gold is Rhamnaceae Rhamnaceae Zao family Trib.Zizipheae Brongn.Gou catechu plant iron-clad gold Berchemia lineate (L.) Dried root of DC., also known as small leaf iron-coated gold, oolong root, Wukouzai, rat milk root, the original plant iron-clad gold is rattan or low shrub, also known as mouse ear, Mira rattan, small leaf Eel vine, born in hills and mountain bushes or on the edge of forests, is distributed in Guangdong Province, Guangxi, Fujian, Hunan, Taiwan and other provinces and regions, and is also distributed in Vietnam, Pakistan, India, Sikkim, and Japan. Its nature and flavor are sweet, light and flat, and it can regulate the lung and relieve cough, dispel blood stasis and relieve pain, soothe the liver and relieve jaundice, invigorate the stomach and eliminate ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 严寒静何梦玲叶燕莹吴碧燕
Owner GUANGDONG PHARMA UNIV
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