The invention relates to
screening method of a pumpkin material for a glossy
disease-resistant
rootstock. The method comprises the following steps: (1)
grafting: collecting different pumpkin
germplasm materials,
grafting cucumber scions to pumpkin seedlings having two true leaves and an apical
bud, pinching the true leaves of
rootstock pumpkins before the
grafting, retaining growing points, planting grafted seedlings, and retaining two vines, a cucumber
vine and a pumpkin
vine; (2) cucumber glossy performance screening: comparing and screening the grafted seedlings, selecting materials with the most obvious cucumber dewaxing
powder and the best brightness, after bearing 2 to 3 cucumbers, removing the cucumber vines in time, only retaining the pumpkin vines to identify and screen
disease resistance character or reserve seeds; (3) pumpkin
rootstock disease resistance screening: screening the grated seedlings for resistance to
fusarium wilt, resistance to
powdery mildew, and resistance to CGMMV,
selfing the materials with resistance to disease and cucumber glossy performance and retaining F1 generation pumpkin seeds. The method has short breeding time, high efficiency and standardized means, and can retain the fine properties of pumpkin
seedling and rootstock at the same time.